PROLIFERATION AND APOPTOSIS IN RAT MODELS OF ORAL SQUAMOUS CELL CARCINOMA INDUCED BY CAPSAICIN EXTRACT NANOPARTICLE GEL
Background: Oral Squamous Cell Carcinoma (OSCC) is highly invasive neoplasm of oral epithelial tissue that is moderately differentiated and tends to metastasize rapidly. Capsaicin, a pungent phytochemical in red peppers, exhibits both anti-proliferative and potential pro-cancer effects on human canc...
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Universitas Airlangga
2023-11-01
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| Series: | Journal of Vocational Health Studies |
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| Online Access: | https://e-journal.unair.ac.id/JVHS/article/view/41565 |
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| author | Pratiwi Nur Widyaningsih Fitri Aniowati Cantika Nadrotan Naim Nova Dwi Anggraeni |
| author_facet | Pratiwi Nur Widyaningsih Fitri Aniowati Cantika Nadrotan Naim Nova Dwi Anggraeni |
| author_sort | Pratiwi Nur Widyaningsih |
| collection | DOAJ |
| description | Background: Oral Squamous Cell Carcinoma (OSCC) is highly invasive neoplasm of oral epithelial tissue that is moderately differentiated and tends to metastasize rapidly. Capsaicin, a pungent phytochemical in red peppers, exhibits both anti-proliferative and potential pro-cancer effects on human cancer cell lines. Purpose: Determine the proliferation and apoptosis between five groups of rats induced OSCC. Method: 5 groups of treatment; C- (untreated rats), C+ (induced by DMBA), E1 (exposed to DMBA and given cisplatin), E2 (induced by DMBA and Capsaicin extract nanoparticle gel with 1% concentration), and E3 (induced by DMBA and Capsaicin extract nanoparticle gel with a concentration of 3.3%). Maceration method was used to obtain Capsaicin extract from green cayenne pepper, then made into nanoparticle gel. Tissue samples were taken after the treatment was completed, then they were pathologicallyobserved histopathological using IHC to assess apoptic activity via the TUNEL method and proliferative activity using PCNA. Result: The proliferative activity in the E1 group had a significant difference compared to the E2 and E3 groups. E2 and E3 were not significantly different (p-value ≥0.05). Apoptotic activity in Group E1 indicated a significant difference from the E2 group and was not significantly different from the E3 group, whereas the E2 and E3 groups were significantly different from each other (p-value ≤0.05). Conclusion: The research showed that Capsaicin nanoparticle gel increased apoptotic activity while decreasing proliferative activity in different treatment groups of the OSCC rat model. |
| format | Article |
| id | doaj-art-1633a3511766436bbce17913d1de36f2 |
| institution | Kabale University |
| issn | 2580-7161 2580-717X |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Vocational Health Studies |
| spelling | doaj-art-1633a3511766436bbce17913d1de36f22025-02-03T00:36:04ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2023-11-017210010610.20473/jvhs.V7.I2.2023.100-10639646PROLIFERATION AND APOPTOSIS IN RAT MODELS OF ORAL SQUAMOUS CELL CARCINOMA INDUCED BY CAPSAICIN EXTRACT NANOPARTICLE GELPratiwi Nur Widyaningsih0https://orcid.org/0000-0003-0067-2600Fitri Aniowati1https://orcid.org/0009-0008-3670-0734Cantika Nadrotan Naim2https://orcid.org/0009-0002-2683-3214Nova Dwi Anggraeni3https://orcid.org/0009-0002-7280-2179Department of Dentistry, Faculty of Medicine, Universitas Jenderal SoedirmanDepartment of Dentistry, Faculty of Medicine, Universitas Jenderal SoedirmanDepartment of Dentistry, Faculty of Medicine, Universitas Jenderal SoedirmanDepartment of Pharmacy, Faculty of Health Science, Universitas Jenderal Soedirman,Background: Oral Squamous Cell Carcinoma (OSCC) is highly invasive neoplasm of oral epithelial tissue that is moderately differentiated and tends to metastasize rapidly. Capsaicin, a pungent phytochemical in red peppers, exhibits both anti-proliferative and potential pro-cancer effects on human cancer cell lines. Purpose: Determine the proliferation and apoptosis between five groups of rats induced OSCC. Method: 5 groups of treatment; C- (untreated rats), C+ (induced by DMBA), E1 (exposed to DMBA and given cisplatin), E2 (induced by DMBA and Capsaicin extract nanoparticle gel with 1% concentration), and E3 (induced by DMBA and Capsaicin extract nanoparticle gel with a concentration of 3.3%). Maceration method was used to obtain Capsaicin extract from green cayenne pepper, then made into nanoparticle gel. Tissue samples were taken after the treatment was completed, then they were pathologicallyobserved histopathological using IHC to assess apoptic activity via the TUNEL method and proliferative activity using PCNA. Result: The proliferative activity in the E1 group had a significant difference compared to the E2 and E3 groups. E2 and E3 were not significantly different (p-value ≥0.05). Apoptotic activity in Group E1 indicated a significant difference from the E2 group and was not significantly different from the E3 group, whereas the E2 and E3 groups were significantly different from each other (p-value ≤0.05). Conclusion: The research showed that Capsaicin nanoparticle gel increased apoptotic activity while decreasing proliferative activity in different treatment groups of the OSCC rat model.https://e-journal.unair.ac.id/JVHS/article/view/41565osccproliferationapoptosiscapsaicin |
| spellingShingle | Pratiwi Nur Widyaningsih Fitri Aniowati Cantika Nadrotan Naim Nova Dwi Anggraeni PROLIFERATION AND APOPTOSIS IN RAT MODELS OF ORAL SQUAMOUS CELL CARCINOMA INDUCED BY CAPSAICIN EXTRACT NANOPARTICLE GEL Journal of Vocational Health Studies oscc proliferation apoptosis capsaicin |
| title | PROLIFERATION AND APOPTOSIS IN RAT MODELS OF ORAL SQUAMOUS CELL CARCINOMA INDUCED BY CAPSAICIN EXTRACT NANOPARTICLE GEL |
| title_full | PROLIFERATION AND APOPTOSIS IN RAT MODELS OF ORAL SQUAMOUS CELL CARCINOMA INDUCED BY CAPSAICIN EXTRACT NANOPARTICLE GEL |
| title_fullStr | PROLIFERATION AND APOPTOSIS IN RAT MODELS OF ORAL SQUAMOUS CELL CARCINOMA INDUCED BY CAPSAICIN EXTRACT NANOPARTICLE GEL |
| title_full_unstemmed | PROLIFERATION AND APOPTOSIS IN RAT MODELS OF ORAL SQUAMOUS CELL CARCINOMA INDUCED BY CAPSAICIN EXTRACT NANOPARTICLE GEL |
| title_short | PROLIFERATION AND APOPTOSIS IN RAT MODELS OF ORAL SQUAMOUS CELL CARCINOMA INDUCED BY CAPSAICIN EXTRACT NANOPARTICLE GEL |
| title_sort | proliferation and apoptosis in rat models of oral squamous cell carcinoma induced by capsaicin extract nanoparticle gel |
| topic | oscc proliferation apoptosis capsaicin |
| url | https://e-journal.unair.ac.id/JVHS/article/view/41565 |
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