The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Chronic periodontitis (CP) is an inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria, and immune mechanisms all play important roles. The aim of this study was to evaluate the occurrence of IL-4 gene polymorphisms in chronic periodontitis and...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/185757 |
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| author | Jirina Bartova Petra Borilova Linhartova Stepan Podzimek Tatjana Janatova Kazi Svobodova Antonin Fassmann Jana Duskova Jaromir Belacek Lydie Izakovicova Holla |
| author_facet | Jirina Bartova Petra Borilova Linhartova Stepan Podzimek Tatjana Janatova Kazi Svobodova Antonin Fassmann Jana Duskova Jaromir Belacek Lydie Izakovicova Holla |
| author_sort | Jirina Bartova |
| collection | DOAJ |
| description | Chronic periodontitis (CP) is an inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria, and immune mechanisms all play important roles. The aim of this study was to evaluate the occurrence of IL-4 gene polymorphisms in chronic periodontitis and to investigate the association between polymorphisms and cytokines production after bacterial stimulation. Sixty-two subjects (47 CP patients and 15 healthy controls) with detected two polymorphisms in the IL-4 gene (-590C/T and intron 3 VNTR) were examined. Production of cytokines (IL-1α, IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, TNFα, INFγ, and VEGF) was studied after in vitro stimulation of isolated peripheral blood by mitogens (Pokeweed mitogen, Concanavalin A), dental plaque bacteria (Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, and Prevotella intermedia), and Heat Shock Protein (HSP) 70 by the Luminex multiplex cytokine analysis system. The results were correlated with IL-4 genotypes in patients with CP and healthy controls. The mononuclear cells isolated from peripheral blood of CP patients with selected IL-4 polymorphisms significantly altered the production of IFNγ, IL-10, IL-1β, IL-1α, TNFα, and IL-6 after stimulation by HSP 70 or selected bacteria (from P<0.001 to P<0.05). IL-4 gene polymorphisms may influence the function of mononuclear cells to produce not only interleukin-4 but also other cytokines, especially in patients with CP. |
| format | Article |
| id | doaj-art-1631223c72ba470f9d4f1987f4987540 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1631223c72ba470f9d4f1987f49875402025-02-03T01:27:48ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/185757185757The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy ControlsJirina Bartova0Petra Borilova Linhartova1Stepan Podzimek2Tatjana Janatova3Kazi Svobodova4Antonin Fassmann5Jana Duskova6Jaromir Belacek7Lydie Izakovicova Holla8Institute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, Karlovo nam. 554/32, 12808 Prague, Czech RepublicDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, 65691 Brno, Czech RepublicInstitute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, Karlovo nam. 554/32, 12808 Prague, Czech RepublicInstitute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, Karlovo nam. 554/32, 12808 Prague, Czech RepublicInstitute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, Karlovo nam. 554/32, 12808 Prague, Czech RepublicClinic of Stomatology, Faculty of Medicine, Masaryk University and St. Anne’s University Hospital, 65691 Brno, Czech RepublicInstitute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, Karlovo nam. 554/32, 12808 Prague, Czech RepublicInstitute of Biophysics and Informatics, First Faculty of Medicine, Charles University, 12108 Prague, Czech RepublicDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, 65691 Brno, Czech RepublicChronic periodontitis (CP) is an inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria, and immune mechanisms all play important roles. The aim of this study was to evaluate the occurrence of IL-4 gene polymorphisms in chronic periodontitis and to investigate the association between polymorphisms and cytokines production after bacterial stimulation. Sixty-two subjects (47 CP patients and 15 healthy controls) with detected two polymorphisms in the IL-4 gene (-590C/T and intron 3 VNTR) were examined. Production of cytokines (IL-1α, IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, TNFα, INFγ, and VEGF) was studied after in vitro stimulation of isolated peripheral blood by mitogens (Pokeweed mitogen, Concanavalin A), dental plaque bacteria (Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, and Prevotella intermedia), and Heat Shock Protein (HSP) 70 by the Luminex multiplex cytokine analysis system. The results were correlated with IL-4 genotypes in patients with CP and healthy controls. The mononuclear cells isolated from peripheral blood of CP patients with selected IL-4 polymorphisms significantly altered the production of IFNγ, IL-10, IL-1β, IL-1α, TNFα, and IL-6 after stimulation by HSP 70 or selected bacteria (from P<0.001 to P<0.05). IL-4 gene polymorphisms may influence the function of mononuclear cells to produce not only interleukin-4 but also other cytokines, especially in patients with CP.http://dx.doi.org/10.1155/2014/185757 |
| spellingShingle | Jirina Bartova Petra Borilova Linhartova Stepan Podzimek Tatjana Janatova Kazi Svobodova Antonin Fassmann Jana Duskova Jaromir Belacek Lydie Izakovicova Holla The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls Mediators of Inflammation |
| title | The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls |
| title_full | The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls |
| title_fullStr | The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls |
| title_full_unstemmed | The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls |
| title_short | The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls |
| title_sort | effect of il 4 gene polymorphisms on cytokine production in patients with chronic periodontitis and in healthy controls |
| url | http://dx.doi.org/10.1155/2014/185757 |
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