Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soil
Background Soil microorganisms are crucial for plant growth, and both plants and their associated rhizosphere microbes are impacted by changes in soil moisture. Inoculation with beneficial fungi can improve bacterial community structure and soil parameters. Aim Under drought stress conditions, the e...
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2025-01-01
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| author | Wei Yin Le Wang Qian Han Shang Yi Xin Li Wei Sa Quan Min Dong Jian Liang |
| author_facet | Wei Yin Le Wang Qian Han Shang Yi Xin Li Wei Sa Quan Min Dong Jian Liang |
| author_sort | Wei Yin |
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| description | Background Soil microorganisms are crucial for plant growth, and both plants and their associated rhizosphere microbes are impacted by changes in soil moisture. Inoculation with beneficial fungi can improve bacterial community structure and soil parameters. Aim Under drought stress conditions, the effects of inoculation with Morchella on the physicochemical properties, enzyme activity, and bacterial community structure of the rhizosphere soil of Poa pratensis were studied. Methods High-throughput sequencing was employed to study rhizosphere soil bacterial communities in both Morchella-inoculated and uninoculated Poa pratensis rhizosphere soil subjected to moderate (50% soil moisture) and severe (30% soil moisture) drought stress, as well as under normal water conditions (70% soil moisture). Results Morchella inoculation significantly increased the alkaline nitrogen (AN) and available phosphorus (AP) contents, protease activity (PA), and alkaline phosphatase activity (APA) of Poa pratensis rhizosphere soil. Both Morchella inoculation and drought stress significantly altered the abundance and diversity of the P. pratensis rhizosphere community. The Chao1, Shannon, and Pielou diversity indices decreased with increasing drought stress. The effect of Morchella inoculation was improved under moderate drought stress and unstressed conditions. In addition, Morchella inoculation may help to stabilize the rhizosphere bacterial community under various levels of soil moisture. |
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| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-161be066c07741fbbd484f2866a760d32025-02-02T15:05:25ZengPeerJ Inc.PeerJ2167-83592025-01-0113e1879310.7717/peerj.18793Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soilWei Yin0Le Wang1Qian Han Shang2Yi Xin Li3Wei Sa4Quan Min Dong5Jian Liang6State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, ChinaBackground Soil microorganisms are crucial for plant growth, and both plants and their associated rhizosphere microbes are impacted by changes in soil moisture. Inoculation with beneficial fungi can improve bacterial community structure and soil parameters. Aim Under drought stress conditions, the effects of inoculation with Morchella on the physicochemical properties, enzyme activity, and bacterial community structure of the rhizosphere soil of Poa pratensis were studied. Methods High-throughput sequencing was employed to study rhizosphere soil bacterial communities in both Morchella-inoculated and uninoculated Poa pratensis rhizosphere soil subjected to moderate (50% soil moisture) and severe (30% soil moisture) drought stress, as well as under normal water conditions (70% soil moisture). Results Morchella inoculation significantly increased the alkaline nitrogen (AN) and available phosphorus (AP) contents, protease activity (PA), and alkaline phosphatase activity (APA) of Poa pratensis rhizosphere soil. Both Morchella inoculation and drought stress significantly altered the abundance and diversity of the P. pratensis rhizosphere community. The Chao1, Shannon, and Pielou diversity indices decreased with increasing drought stress. The effect of Morchella inoculation was improved under moderate drought stress and unstressed conditions. In addition, Morchella inoculation may help to stabilize the rhizosphere bacterial community under various levels of soil moisture.https://peerj.com/articles/18793.pdfDrought-stressEnzyme activityMorchella inoculationPoa pratensis L.Rhizosphere bacterial communitySoil physicochemical properties |
| spellingShingle | Wei Yin Le Wang Qian Han Shang Yi Xin Li Wei Sa Quan Min Dong Jian Liang Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soil PeerJ Drought-stress Enzyme activity Morchella inoculation Poa pratensis L. Rhizosphere bacterial community Soil physicochemical properties |
| title | Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soil |
| title_full | Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soil |
| title_fullStr | Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soil |
| title_full_unstemmed | Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soil |
| title_short | Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soil |
| title_sort | effects of drought stress and morchella inoculation on the physicochemical properties enzymatic activities and bacterial community of poa pratensis l rhizosphere soil |
| topic | Drought-stress Enzyme activity Morchella inoculation Poa pratensis L. Rhizosphere bacterial community Soil physicochemical properties |
| url | https://peerj.com/articles/18793.pdf |
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