Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells
Plasma cells produce and secrete massive amounts of disulfide-containing antibodies. To accommodate this load on the secretory machinery, the differentiation of resting B cells into antibody-secreting plasma cells is accompanied by a preferential expansion of the secretory compartments of the cells...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2013/898563 |
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| author | Rosa E. Hansen Mieko Otsu Ineke Braakman Jakob R. Winther |
| author_facet | Rosa E. Hansen Mieko Otsu Ineke Braakman Jakob R. Winther |
| author_sort | Rosa E. Hansen |
| collection | DOAJ |
| description | Plasma cells produce and secrete massive amounts of disulfide-containing antibodies. To accommodate this load on the secretory machinery, the differentiation of resting B cells into antibody-secreting plasma cells is accompanied by a preferential expansion of the secretory compartments of the cells and by an up-regulation of enzymes involved in redox regulation and protein folding. We have quantified the absolute levels of protein thiols, protein disulfides, and glutathionylated proteins in whole cells. The results show that while the global thiol-disulfide state is affected to some extent by the differentiation, steady-state levels of glutathionylated protein thiols are less than 0.3% of the total protein cysteines, even in fully differentiated cells, and the overall protein redox state is not affected until late in differentiation, when large-scale IgM production is ongoing. A general expansion of the ER does not affect global protein redox status until an extensive production of cargo proteins has started. |
| format | Article |
| id | doaj-art-161b36fe6842444a846c9fa1f6b11367 |
| institution | Kabale University |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-161b36fe6842444a846c9fa1f6b113672025-02-03T06:13:14ZengWileyInternational Journal of Cell Biology1687-88761687-88842013-01-01201310.1155/2013/898563898563Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma CellsRosa E. Hansen0Mieko Otsu1Ineke Braakman2Jakob R. Winther3Section for Biomolecular Sciences, Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200 Copenhagen, DenmarkCellular Protein Chemistry, Bijvoet Center for Biomolecular Research, Faculty of Science, Utrecht University, The NetherlandsCellular Protein Chemistry, Bijvoet Center for Biomolecular Research, Faculty of Science, Utrecht University, The NetherlandsSection for Biomolecular Sciences, Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200 Copenhagen, DenmarkPlasma cells produce and secrete massive amounts of disulfide-containing antibodies. To accommodate this load on the secretory machinery, the differentiation of resting B cells into antibody-secreting plasma cells is accompanied by a preferential expansion of the secretory compartments of the cells and by an up-regulation of enzymes involved in redox regulation and protein folding. We have quantified the absolute levels of protein thiols, protein disulfides, and glutathionylated proteins in whole cells. The results show that while the global thiol-disulfide state is affected to some extent by the differentiation, steady-state levels of glutathionylated protein thiols are less than 0.3% of the total protein cysteines, even in fully differentiated cells, and the overall protein redox state is not affected until late in differentiation, when large-scale IgM production is ongoing. A general expansion of the ER does not affect global protein redox status until an extensive production of cargo proteins has started.http://dx.doi.org/10.1155/2013/898563 |
| spellingShingle | Rosa E. Hansen Mieko Otsu Ineke Braakman Jakob R. Winther Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells International Journal of Cell Biology |
| title | Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells |
| title_full | Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells |
| title_fullStr | Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells |
| title_full_unstemmed | Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells |
| title_short | Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells |
| title_sort | quantifying changes in the cellular thiol disulfide status during differentiation of b cells into antibody secreting plasma cells |
| url | http://dx.doi.org/10.1155/2013/898563 |
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