Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria
The effects of phosphate-solubilizing bacteria (PSB) on plant productivity are high variable under field conditions. Soil phosphorus (P) levels are proposed to impact PSB performance. Furthermore, the effect of exogenous PSB on rhizosphere microbial community and their functions are largely unexplor...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1538117/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832583764498186240 |
|---|---|
| author | Yuhan Tang Linlin Wang Jing Fu Fangyuan Zhou Hailei Wei Xiaoqing Wu Susu Fan Xinjian Zhang |
| author_facet | Yuhan Tang Linlin Wang Jing Fu Fangyuan Zhou Hailei Wei Xiaoqing Wu Susu Fan Xinjian Zhang |
| author_sort | Yuhan Tang |
| collection | DOAJ |
| description | The effects of phosphate-solubilizing bacteria (PSB) on plant productivity are high variable under field conditions. Soil phosphorus (P) levels are proposed to impact PSB performance. Furthermore, the effect of exogenous PSB on rhizosphere microbial community and their functions are largely unexplored. Our study examined how different P background and fertilization affected the performance of PSB Pseudomonas asiatica JP233. We further conducted metagenomic sequencing to assess its impact on rhizosphere microbiota and functions, with a focus on genes related to soil P cycling. We found that JP233 could enhance P solubilization and tomato growth to different extent in both high and low P soils, irrespective of P fertilization. It was particularly effective in high P soil without extra fertilization. JP233 altered the rhizosphere microbial community, boosting taxa known for plant growth promotion. It also changed soil gene profiling, enriching pathways related to secondary metabolite biosynthesis, amino acids, carbon metabolism, and other key processes. Particularly, JP233 increased the abundance of most P cycle genes and strengthened their interconnections. Populations of certain predatory bacteria increased after JP233 inoculation. Our findings provide valuable insights into PSB’s mechanisms for P solubilization and plant growth promotion, as well as potential adverse impacts of resident microbes on bioinoculants. |
| format | Article |
| id | doaj-art-15ec37162b054748baffbb8e498ca762 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-15ec37162b054748baffbb8e498ca7622025-01-28T06:41:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.15381171538117Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteriaYuhan Tang0Linlin Wang1Jing Fu2Fangyuan Zhou3Hailei Wei4Xiaoqing Wu5Susu Fan6Xinjian Zhang7Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, ChinaKey Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, ChinaThe effects of phosphate-solubilizing bacteria (PSB) on plant productivity are high variable under field conditions. Soil phosphorus (P) levels are proposed to impact PSB performance. Furthermore, the effect of exogenous PSB on rhizosphere microbial community and their functions are largely unexplored. Our study examined how different P background and fertilization affected the performance of PSB Pseudomonas asiatica JP233. We further conducted metagenomic sequencing to assess its impact on rhizosphere microbiota and functions, with a focus on genes related to soil P cycling. We found that JP233 could enhance P solubilization and tomato growth to different extent in both high and low P soils, irrespective of P fertilization. It was particularly effective in high P soil without extra fertilization. JP233 altered the rhizosphere microbial community, boosting taxa known for plant growth promotion. It also changed soil gene profiling, enriching pathways related to secondary metabolite biosynthesis, amino acids, carbon metabolism, and other key processes. Particularly, JP233 increased the abundance of most P cycle genes and strengthened their interconnections. Populations of certain predatory bacteria increased after JP233 inoculation. Our findings provide valuable insights into PSB’s mechanisms for P solubilization and plant growth promotion, as well as potential adverse impacts of resident microbes on bioinoculants.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1538117/fullphosphate-solubilizing bacteriaPseudomonas asiaticasoil P cyclingmetagenomicspredatory bacteria |
| spellingShingle | Yuhan Tang Linlin Wang Jing Fu Fangyuan Zhou Hailei Wei Xiaoqing Wu Susu Fan Xinjian Zhang Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria Frontiers in Microbiology phosphate-solubilizing bacteria Pseudomonas asiatica soil P cycling metagenomics predatory bacteria |
| title | Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria |
| title_full | Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria |
| title_fullStr | Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria |
| title_full_unstemmed | Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria |
| title_short | Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria |
| title_sort | unraveling the microecological mechanisms of phosphate solubilizing pseudomonas asiatica jp233 through metagenomics insights into the roles of rhizosphere microbiota and predatory bacteria |
| topic | phosphate-solubilizing bacteria Pseudomonas asiatica soil P cycling metagenomics predatory bacteria |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1538117/full |
| work_keys_str_mv | AT yuhantang unravelingthemicroecologicalmechanismsofphosphatesolubilizingpseudomonasasiaticajp233throughmetagenomicsinsightsintotherolesofrhizospheremicrobiotaandpredatorybacteria AT linlinwang unravelingthemicroecologicalmechanismsofphosphatesolubilizingpseudomonasasiaticajp233throughmetagenomicsinsightsintotherolesofrhizospheremicrobiotaandpredatorybacteria AT jingfu unravelingthemicroecologicalmechanismsofphosphatesolubilizingpseudomonasasiaticajp233throughmetagenomicsinsightsintotherolesofrhizospheremicrobiotaandpredatorybacteria AT fangyuanzhou unravelingthemicroecologicalmechanismsofphosphatesolubilizingpseudomonasasiaticajp233throughmetagenomicsinsightsintotherolesofrhizospheremicrobiotaandpredatorybacteria AT haileiwei unravelingthemicroecologicalmechanismsofphosphatesolubilizingpseudomonasasiaticajp233throughmetagenomicsinsightsintotherolesofrhizospheremicrobiotaandpredatorybacteria AT xiaoqingwu unravelingthemicroecologicalmechanismsofphosphatesolubilizingpseudomonasasiaticajp233throughmetagenomicsinsightsintotherolesofrhizospheremicrobiotaandpredatorybacteria AT susufan unravelingthemicroecologicalmechanismsofphosphatesolubilizingpseudomonasasiaticajp233throughmetagenomicsinsightsintotherolesofrhizospheremicrobiotaandpredatorybacteria AT xinjianzhang unravelingthemicroecologicalmechanismsofphosphatesolubilizingpseudomonasasiaticajp233throughmetagenomicsinsightsintotherolesofrhizospheremicrobiotaandpredatorybacteria |