Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced Rats
It is speculated that thyroid hormones may be involved in nonalcoholic fatty liver disease (NAFLD) pathogenesis. A literature scan, however, demonstrated conflicting results from studies investigating the relationship between hypothyroidism and NAFLD. Therefore, our study aims to evaluate NAFLD, fro...
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Wiley
2016-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2016/5083746 |
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| author | Şule Demir Mustafa Ünübol Serap Ünübol Aypak Emrah İpek Serdar Aktaş Gamze Sevri Ekren Murat Yılmaz Recai Tunca Engin Güney |
| author_facet | Şule Demir Mustafa Ünübol Serap Ünübol Aypak Emrah İpek Serdar Aktaş Gamze Sevri Ekren Murat Yılmaz Recai Tunca Engin Güney |
| author_sort | Şule Demir |
| collection | DOAJ |
| description | It is speculated that thyroid hormones may be involved in nonalcoholic fatty liver disease (NAFLD) pathogenesis. A literature scan, however, demonstrated conflicting results from studies investigating the relationship between hypothyroidism and NAFLD. Therefore, our study aims to evaluate NAFLD, from the histopathologic perspective, in hypothyroidism-induced rats. Wistar rats were divided into 2 groups: the experimental group consumed water containing methimazole 0.025% (MMI, Sigma, USA) for 12 weeks and the control group consumed tap water. At the end of week 12, serum glucose, ALT, AST, triglyceride, HDL, LDL, TSH, fT4, fT3, visfatin, and insulin assays were performed. Sections were stained with hematoxylin-eosin and “Oil Red-O” for histopathologic examination of the livers. In our study, we detected mild hepatosteatosis in all hypothyroidism-induced rats. There was statistically significant difference with respect to obesity between the two groups (p<0.001). The mean fasting blood glucose was 126.25 ± 23.4 mg/dL in hypothyroidism-induced group and 102.63 ± 15.51 mg/dL in the control group, with a statistically significant difference between the groups (p=0.032). The two groups did not differ statistically significantly with respect to visfatin levels (p>0.05). In conclusion, we found that hypothyroidism-induced rats had mild hepatosteatosis as opposed to the control group histopathologically. Our study indicates that hypothyroidism can cause NAFLD. |
| format | Article |
| id | doaj-art-15c71e5b538444aab811c70ae3bf8f43 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-15c71e5b538444aab811c70ae3bf8f432025-02-03T06:12:16ZengWileyInternational Journal of Endocrinology1687-83371687-83452016-01-01201610.1155/2016/50837465083746Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced RatsŞule Demir0Mustafa Ünübol1Serap Ünübol Aypak2Emrah İpek3Serdar Aktaş4Gamze Sevri Ekren5Murat Yılmaz6Recai Tunca7Engin Güney8Department of Internal Medicine, Adnan Menderes University Faculty of Medicine, 09100 Aydin, TurkeyDivision of Endocrinology, Department of Internal Medicine, Adnan Menderes University Faculty of Medicine, 09100 Aydın, TurkeyDepartment of Biochemistry, Adnan Menderes University Faculty of Veterinary Medicine, 09100 Aydın, TurkeyDepartment of Pathology, Adnan Menderes University Faculty of Veterinary Medicine, 09100 Aydın, TurkeyDepartment of Pharmacology Toxicology, Adnan Menderes University Faculty of Veterinary Medicine, 09100 Aydın, TurkeyDepartment of Biochemistry, Adnan Menderes University Faculty of Veterinary Medicine, 09100 Aydın, TurkeyDepartment of General Surgery, Adnan Menderes University Faculty of Medicine, 09100 Aydın, TurkeyDepartment of Pathology, Adnan Menderes University Faculty of Veterinary Medicine, 09100 Aydın, TurkeyDivision of Endocrinology, Department of Internal Medicine, Adnan Menderes University Faculty of Medicine, 09100 Aydın, TurkeyIt is speculated that thyroid hormones may be involved in nonalcoholic fatty liver disease (NAFLD) pathogenesis. A literature scan, however, demonstrated conflicting results from studies investigating the relationship between hypothyroidism and NAFLD. Therefore, our study aims to evaluate NAFLD, from the histopathologic perspective, in hypothyroidism-induced rats. Wistar rats were divided into 2 groups: the experimental group consumed water containing methimazole 0.025% (MMI, Sigma, USA) for 12 weeks and the control group consumed tap water. At the end of week 12, serum glucose, ALT, AST, triglyceride, HDL, LDL, TSH, fT4, fT3, visfatin, and insulin assays were performed. Sections were stained with hematoxylin-eosin and “Oil Red-O” for histopathologic examination of the livers. In our study, we detected mild hepatosteatosis in all hypothyroidism-induced rats. There was statistically significant difference with respect to obesity between the two groups (p<0.001). The mean fasting blood glucose was 126.25 ± 23.4 mg/dL in hypothyroidism-induced group and 102.63 ± 15.51 mg/dL in the control group, with a statistically significant difference between the groups (p=0.032). The two groups did not differ statistically significantly with respect to visfatin levels (p>0.05). In conclusion, we found that hypothyroidism-induced rats had mild hepatosteatosis as opposed to the control group histopathologically. Our study indicates that hypothyroidism can cause NAFLD.http://dx.doi.org/10.1155/2016/5083746 |
| spellingShingle | Şule Demir Mustafa Ünübol Serap Ünübol Aypak Emrah İpek Serdar Aktaş Gamze Sevri Ekren Murat Yılmaz Recai Tunca Engin Güney Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced Rats International Journal of Endocrinology |
| title | Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced Rats |
| title_full | Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced Rats |
| title_fullStr | Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced Rats |
| title_full_unstemmed | Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced Rats |
| title_short | Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced Rats |
| title_sort | histopathologic evaluation of nonalcoholic fatty liver disease in hypothyroidism induced rats |
| url | http://dx.doi.org/10.1155/2016/5083746 |
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