De novo transcriptome assembly of the Mediterranean sea-rock pool mosquitoes Aedes mariae and Aedes zammitii
Abstract Understanding the genomic consequences of hybridization is an essential research focus in global change biology. Species adapted to rapidly changing environments can offer valuable, yet largely underexplored insights in this context. Here, we present the first de novo transcriptomes of the...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04393-2 |
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| author | Valentina Mastrantonio Daniele Porretta Franco Liberati Roberta Bisconti Tiziana Castrignanò Daniele Canestrelli |
| author_facet | Valentina Mastrantonio Daniele Porretta Franco Liberati Roberta Bisconti Tiziana Castrignanò Daniele Canestrelli |
| author_sort | Valentina Mastrantonio |
| collection | DOAJ |
| description | Abstract Understanding the genomic consequences of hybridization is an essential research focus in global change biology. Species adapted to rapidly changing environments can offer valuable, yet largely underexplored insights in this context. Here, we present the first de novo transcriptomes of the sea-rock pools mosquitoes Aedes mariae and Aedes zammitii, two species adapted to highly variable habitats. Using RNA-seq data obtained from larval stages, we assembled and annotated 95,059,578 reads for Ae. mariae and 101,050,236 reads for Ae. zammitii, detecting 49,352 transcripts with N50 of 2,615 for the former and 43,461 transcripts with N50 of 2,570 for the latter. Validation by BUSCO confirmed the high quality of our resources. Homology alignments of predicted ORFs showed that 21,842 sequences from Ae. mariae and 21,944 sequences from Ae. zammitii mapped to the Nr, SwissProt, and TrEMBL databases, while 19,208 and 19,393 predicted ORFs, respectively, were functionally annotated using the COG and KEGG databases. These high-quality transcriptomes will provide valuable resources to investigate the role of hybridization in species adaptation to changing environments. |
| format | Article |
| id | doaj-art-15c0e2dd6fd84e7eb2500e4cbd4e13f6 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Data |
| spelling | doaj-art-15c0e2dd6fd84e7eb2500e4cbd4e13f62025-01-26T12:14:32ZengNature PortfolioScientific Data2052-44632025-01-0112111310.1038/s41597-025-04393-2De novo transcriptome assembly of the Mediterranean sea-rock pool mosquitoes Aedes mariae and Aedes zammitiiValentina Mastrantonio0Daniele Porretta1Franco Liberati2Roberta Bisconti3Tiziana Castrignanò4Daniele Canestrelli5Department of Environmental Biology, Sapienza University of RomeDepartment of Environmental Biology, Sapienza University of RomeDepartment of Ecological and Biological Sciences (DEB), Tuscia UniversityDepartment of Ecological and Biological Sciences (DEB), Tuscia UniversityDepartment of Ecological and Biological Sciences (DEB), Tuscia UniversityDepartment of Ecological and Biological Sciences (DEB), Tuscia UniversityAbstract Understanding the genomic consequences of hybridization is an essential research focus in global change biology. Species adapted to rapidly changing environments can offer valuable, yet largely underexplored insights in this context. Here, we present the first de novo transcriptomes of the sea-rock pools mosquitoes Aedes mariae and Aedes zammitii, two species adapted to highly variable habitats. Using RNA-seq data obtained from larval stages, we assembled and annotated 95,059,578 reads for Ae. mariae and 101,050,236 reads for Ae. zammitii, detecting 49,352 transcripts with N50 of 2,615 for the former and 43,461 transcripts with N50 of 2,570 for the latter. Validation by BUSCO confirmed the high quality of our resources. Homology alignments of predicted ORFs showed that 21,842 sequences from Ae. mariae and 21,944 sequences from Ae. zammitii mapped to the Nr, SwissProt, and TrEMBL databases, while 19,208 and 19,393 predicted ORFs, respectively, were functionally annotated using the COG and KEGG databases. These high-quality transcriptomes will provide valuable resources to investigate the role of hybridization in species adaptation to changing environments.https://doi.org/10.1038/s41597-025-04393-2 |
| spellingShingle | Valentina Mastrantonio Daniele Porretta Franco Liberati Roberta Bisconti Tiziana Castrignanò Daniele Canestrelli De novo transcriptome assembly of the Mediterranean sea-rock pool mosquitoes Aedes mariae and Aedes zammitii Scientific Data |
| title | De novo transcriptome assembly of the Mediterranean sea-rock pool mosquitoes Aedes mariae and Aedes zammitii |
| title_full | De novo transcriptome assembly of the Mediterranean sea-rock pool mosquitoes Aedes mariae and Aedes zammitii |
| title_fullStr | De novo transcriptome assembly of the Mediterranean sea-rock pool mosquitoes Aedes mariae and Aedes zammitii |
| title_full_unstemmed | De novo transcriptome assembly of the Mediterranean sea-rock pool mosquitoes Aedes mariae and Aedes zammitii |
| title_short | De novo transcriptome assembly of the Mediterranean sea-rock pool mosquitoes Aedes mariae and Aedes zammitii |
| title_sort | de novo transcriptome assembly of the mediterranean sea rock pool mosquitoes aedes mariae and aedes zammitii |
| url | https://doi.org/10.1038/s41597-025-04393-2 |
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