Reproductive effects of the tumor necrosis factor (TNF) deficiency in mice
TNF is a multifunctional cytokine that, at physiological concentrations, maintains the balance between apoptosis and survival of male germ cells and, at higher concentrations, has adverse effects on various stages of the reproductive process. Although ant-cytokine therapies have been used in million...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/426 |
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| author | S. O. Maslennikova G. V. Kontsevaya M. A. Zolotykh M. V. Anisimova N. A. Feofanova M. P. Moshkin S. A. Nedospasov L. A. Gerlinskaya |
| author_facet | S. O. Maslennikova G. V. Kontsevaya M. A. Zolotykh M. V. Anisimova N. A. Feofanova M. P. Moshkin S. A. Nedospasov L. A. Gerlinskaya |
| author_sort | S. O. Maslennikova |
| collection | DOAJ |
| description | TNF is a multifunctional cytokine that, at physiological concentrations, maintains the balance between apoptosis and survival of male germ cells and, at higher concentrations, has adverse effects on various stages of the reproductive process. Although ant-cytokine therapies have been used in millions of patients, the consequences of cytokine deficiency for reproductive functions are poorly understood and need attention. In this work, we have studied behavioral interactions between males and females, spermatogenesis, male fertility, and embryonic developmental characteristics of the progeny in TNFα knockout mice (TNF-/-). We have demonstrated that TNF is involved in the regulation of sexual behavior, spermatogenesis, pre- and postimplantation development. Complete TNF deficiency led to decreased reproductive efficiency: a lower number of viable embryos were observed in TNF-/- mice than in wild-type mice. The decrease in fertility was caused by preimplantation embryo loss in TNF-/- mice. Preimplantation loss in females might be caused by asospermia in TNF-/- males. Additionally, the sensitivity of reproductive functions to female stimuli was different between TNF-/- mice and wild-type mice, while interactions with females increased the concentrations of spermatozoids in both TNF-/- and wild-type mice. Still higher levels were observed in knockout animals, which led to increase in the number of immature spermatozoids in epididymides. |
| format | Article |
| id | doaj-art-15bd0b3f0cb2479b83c46071eb00b3cf |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-15bd0b3f0cb2479b83c46071eb00b3cf2025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-12-0119440440910.18699/VJ15.052385Reproductive effects of the tumor necrosis factor (TNF) deficiency in miceS. O. Maslennikova0G. V. Kontsevaya1M. A. Zolotykh2M. V. Anisimova3N. A. Feofanova4M. P. Moshkin5S. A. Nedospasov6L. A. Gerlinskaya7Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaEngelhardt Institute of Molecular Biology Russian Academy of Sciences, Moscow, Russia Lomonosov Moscow State University, Moscow, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaTNF is a multifunctional cytokine that, at physiological concentrations, maintains the balance between apoptosis and survival of male germ cells and, at higher concentrations, has adverse effects on various stages of the reproductive process. Although ant-cytokine therapies have been used in millions of patients, the consequences of cytokine deficiency for reproductive functions are poorly understood and need attention. In this work, we have studied behavioral interactions between males and females, spermatogenesis, male fertility, and embryonic developmental characteristics of the progeny in TNFα knockout mice (TNF-/-). We have demonstrated that TNF is involved in the regulation of sexual behavior, spermatogenesis, pre- and postimplantation development. Complete TNF deficiency led to decreased reproductive efficiency: a lower number of viable embryos were observed in TNF-/- mice than in wild-type mice. The decrease in fertility was caused by preimplantation embryo loss in TNF-/- mice. Preimplantation loss in females might be caused by asospermia in TNF-/- males. Additionally, the sensitivity of reproductive functions to female stimuli was different between TNF-/- mice and wild-type mice, while interactions with females increased the concentrations of spermatozoids in both TNF-/- and wild-type mice. Still higher levels were observed in knockout animals, which led to increase in the number of immature spermatozoids in epididymides.https://vavilov.elpub.ru/jour/article/view/426tumor necrosis factor (tnf)tnf knockout micespermatogenesispre- and postnatal developmentsexual behavior |
| spellingShingle | S. O. Maslennikova G. V. Kontsevaya M. A. Zolotykh M. V. Anisimova N. A. Feofanova M. P. Moshkin S. A. Nedospasov L. A. Gerlinskaya Reproductive effects of the tumor necrosis factor (TNF) deficiency in mice Вавиловский журнал генетики и селекции tumor necrosis factor (tnf) tnf knockout mice spermatogenesis pre- and postnatal development sexual behavior |
| title | Reproductive effects of the tumor necrosis factor (TNF) deficiency in mice |
| title_full | Reproductive effects of the tumor necrosis factor (TNF) deficiency in mice |
| title_fullStr | Reproductive effects of the tumor necrosis factor (TNF) deficiency in mice |
| title_full_unstemmed | Reproductive effects of the tumor necrosis factor (TNF) deficiency in mice |
| title_short | Reproductive effects of the tumor necrosis factor (TNF) deficiency in mice |
| title_sort | reproductive effects of the tumor necrosis factor tnf deficiency in mice |
| topic | tumor necrosis factor (tnf) tnf knockout mice spermatogenesis pre- and postnatal development sexual behavior |
| url | https://vavilov.elpub.ru/jour/article/view/426 |
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