Prolonged Helium Postconditioning Protocols during Early Reperfusion Do Not Induce Cardioprotection in the Rat Heart In Vivo: Role of Inflammatory Cytokines
Postconditioning of myocardial tissue employs short cycles of ischemia or pharmacologic agents during early reperfusion. Effects of helium postconditioning protocols on infarct size and the ischemia/reperfusion-induced immune response were investigated by measurement of protein and mRNA levels of pr...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/216798 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553313913012224 |
|---|---|
| author | Gezina Tanya Mei Ling Oei Hamid Aslami Raphaela Priscilla Kerindongo Renske Johanna Steenstra Charlotte Jacqueline Peter Beurskens Anita Maria Tuip-de Boer Nicole Petra Juffermans Markus Werner Hollmann Benedikt Preckel Nina Claudia Weber |
| author_facet | Gezina Tanya Mei Ling Oei Hamid Aslami Raphaela Priscilla Kerindongo Renske Johanna Steenstra Charlotte Jacqueline Peter Beurskens Anita Maria Tuip-de Boer Nicole Petra Juffermans Markus Werner Hollmann Benedikt Preckel Nina Claudia Weber |
| author_sort | Gezina Tanya Mei Ling Oei |
| collection | DOAJ |
| description | Postconditioning of myocardial tissue employs short cycles of ischemia or pharmacologic agents during early reperfusion. Effects of helium postconditioning protocols on infarct size and the ischemia/reperfusion-induced immune response were investigated by measurement of protein and mRNA levels of proinflammatory cytokines. Rats were anesthetized with S-ketamine (150 mg/kg) and diazepam (1.5 mg/kg). Regional myocardial ischemia/reperfusion was induced; additional groups inhaled 15, 30, or 60 min of 70% helium during reperfusion. Fifteen minutes of helium reduced infarct size from 43% in control to 21%, whereas 30 and 60 minutes of helium inhalation led to an infarct size of 47% and 39%, respectively. Increased protein levels of cytokine-induced neutrophil chemoattractant (CINC-3) and interleukin-1 beta (IL-1β) were found after 30 or 60 min of helium inhalation, in comparison to control. 30 min of helium increased mRNA levels of CINC-3, IL-1β, interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) in myocardial tissue not directly subjected to ischemia/reperfusion. These results suggest that the effectiveness of the helium postconditioning protocol is very sensitive to duration of noble gas application. Additionally, helium was associated with higher levels of inflammatory cytokines; however, it is not clear whether this is causative of nature or part of an epiphenomenon. |
| format | Article |
| id | doaj-art-15aefb907d304116a7fbee317af39218 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-15aefb907d304116a7fbee317af392182025-02-03T05:54:22ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/216798216798Prolonged Helium Postconditioning Protocols during Early Reperfusion Do Not Induce Cardioprotection in the Rat Heart In Vivo: Role of Inflammatory CytokinesGezina Tanya Mei Ling Oei0Hamid Aslami1Raphaela Priscilla Kerindongo2Renske Johanna Steenstra3Charlotte Jacqueline Peter Beurskens4Anita Maria Tuip-de Boer5Nicole Petra Juffermans6Markus Werner Hollmann7Benedikt Preckel8Nina Claudia Weber9Laboratory of Experimental Anesthesiology and Intensive Care, Department of Anesthesiology, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsLaboratory of Experimental Anesthesiology and Intensive Care, Department of Intensive Care, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsLaboratory of Experimental Anesthesiology and Intensive Care, Department of Anesthesiology, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsLaboratory of Experimental Anesthesiology and Intensive Care, Department of Anesthesiology, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsLaboratory of Experimental Anesthesiology and Intensive Care, Department of Intensive Care, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsLaboratory of Experimental Anesthesiology and Intensive Care, Department of Intensive Care, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsLaboratory of Experimental Anesthesiology and Intensive Care, Department of Intensive Care, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsLaboratory of Experimental Anesthesiology and Intensive Care, Department of Anesthesiology, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsLaboratory of Experimental Anesthesiology and Intensive Care, Department of Anesthesiology, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsLaboratory of Experimental Anesthesiology and Intensive Care, Department of Anesthesiology, Academic Medical Centre, Meibergdreef 9, 1100 DD Amsterdam, NetherlandsPostconditioning of myocardial tissue employs short cycles of ischemia or pharmacologic agents during early reperfusion. Effects of helium postconditioning protocols on infarct size and the ischemia/reperfusion-induced immune response were investigated by measurement of protein and mRNA levels of proinflammatory cytokines. Rats were anesthetized with S-ketamine (150 mg/kg) and diazepam (1.5 mg/kg). Regional myocardial ischemia/reperfusion was induced; additional groups inhaled 15, 30, or 60 min of 70% helium during reperfusion. Fifteen minutes of helium reduced infarct size from 43% in control to 21%, whereas 30 and 60 minutes of helium inhalation led to an infarct size of 47% and 39%, respectively. Increased protein levels of cytokine-induced neutrophil chemoattractant (CINC-3) and interleukin-1 beta (IL-1β) were found after 30 or 60 min of helium inhalation, in comparison to control. 30 min of helium increased mRNA levels of CINC-3, IL-1β, interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) in myocardial tissue not directly subjected to ischemia/reperfusion. These results suggest that the effectiveness of the helium postconditioning protocol is very sensitive to duration of noble gas application. Additionally, helium was associated with higher levels of inflammatory cytokines; however, it is not clear whether this is causative of nature or part of an epiphenomenon.http://dx.doi.org/10.1155/2015/216798 |
| spellingShingle | Gezina Tanya Mei Ling Oei Hamid Aslami Raphaela Priscilla Kerindongo Renske Johanna Steenstra Charlotte Jacqueline Peter Beurskens Anita Maria Tuip-de Boer Nicole Petra Juffermans Markus Werner Hollmann Benedikt Preckel Nina Claudia Weber Prolonged Helium Postconditioning Protocols during Early Reperfusion Do Not Induce Cardioprotection in the Rat Heart In Vivo: Role of Inflammatory Cytokines Journal of Immunology Research |
| title | Prolonged Helium Postconditioning Protocols during Early Reperfusion Do Not Induce Cardioprotection in the Rat Heart In Vivo: Role of Inflammatory Cytokines |
| title_full | Prolonged Helium Postconditioning Protocols during Early Reperfusion Do Not Induce Cardioprotection in the Rat Heart In Vivo: Role of Inflammatory Cytokines |
| title_fullStr | Prolonged Helium Postconditioning Protocols during Early Reperfusion Do Not Induce Cardioprotection in the Rat Heart In Vivo: Role of Inflammatory Cytokines |
| title_full_unstemmed | Prolonged Helium Postconditioning Protocols during Early Reperfusion Do Not Induce Cardioprotection in the Rat Heart In Vivo: Role of Inflammatory Cytokines |
| title_short | Prolonged Helium Postconditioning Protocols during Early Reperfusion Do Not Induce Cardioprotection in the Rat Heart In Vivo: Role of Inflammatory Cytokines |
| title_sort | prolonged helium postconditioning protocols during early reperfusion do not induce cardioprotection in the rat heart in vivo role of inflammatory cytokines |
| url | http://dx.doi.org/10.1155/2015/216798 |
| work_keys_str_mv | AT gezinatanyameilingoei prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines AT hamidaslami prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines AT raphaelapriscillakerindongo prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines AT renskejohannasteenstra prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines AT charlottejacquelinepeterbeurskens prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines AT anitamariatuipdeboer prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines AT nicolepetrajuffermans prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines AT markuswernerhollmann prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines AT benediktpreckel prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines AT ninaclaudiaweber prolongedheliumpostconditioningprotocolsduringearlyreperfusiondonotinducecardioprotectionintheratheartinvivoroleofinflammatorycytokines |