Transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol-labeled RNA sequencing
Both acute and chronic stress have significant impact on brain functions. The amygdala is essential in mediating stress responses, but how its transcriptomic dynamics change under stress remains elusive. To overcome the difficulties in detecting subtle stress-induced changes by evaluating total RNA...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-11-01
|
| Series: | Neurobiology of Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352289524000845 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850218936562352128 |
|---|---|
| author | Dan Zhao Lu Zhang Yang Yang |
| author_facet | Dan Zhao Lu Zhang Yang Yang |
| author_sort | Dan Zhao |
| collection | DOAJ |
| description | Both acute and chronic stress have significant impact on brain functions. The amygdala is essential in mediating stress responses, but how its transcriptomic dynamics change under stress remains elusive. To overcome the difficulties in detecting subtle stress-induced changes by evaluating total RNA using classic RNA sequencing, we conducted thiol-labeled RNA sequencing (SLAM-seq). We injected 4-thiouridine (4sU) into mouse amygdala followed by SLAM-seq to detect nascent mRNA induced by acute and chronic restraint stress, and found that SLAM-seq could label actively transcribed genes in the major neuronal and glial subtypes. Using SLAM-seq, we found that chronic stress led to higher turnover of a group of genes associated with myelination, and this finding is confirmed by immunostaining which showed increased myelination in the chronically stressed amygdala. Additionally, genes detected by SLAM-seq and RNA-seq only partially overlapped, suggesting that SLAM-seq and RNA-seq are complementary in identifying stress-responsive genes. By applying SLAM-seq in vivo, we obtained a rich dataset of genes with higher turnover in the amygdala under stress. |
| format | Article |
| id | doaj-art-154f0c97cd1f4de5950179914c0fd5f8 |
| institution | OA Journals |
| issn | 2352-2895 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Stress |
| spelling | doaj-art-154f0c97cd1f4de5950179914c0fd5f82025-08-20T02:07:34ZengElsevierNeurobiology of Stress2352-28952024-11-013310068810.1016/j.ynstr.2024.100688Transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol-labeled RNA sequencingDan Zhao0Lu Zhang1Yang Yang2School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, ChinaSchool of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, ChinaSchool of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; State Key Laboratory of Advanced Medical Materials and Devices, ShanghaiTech University, Shanghai, 201210, China; Corresponding author. School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.Both acute and chronic stress have significant impact on brain functions. The amygdala is essential in mediating stress responses, but how its transcriptomic dynamics change under stress remains elusive. To overcome the difficulties in detecting subtle stress-induced changes by evaluating total RNA using classic RNA sequencing, we conducted thiol-labeled RNA sequencing (SLAM-seq). We injected 4-thiouridine (4sU) into mouse amygdala followed by SLAM-seq to detect nascent mRNA induced by acute and chronic restraint stress, and found that SLAM-seq could label actively transcribed genes in the major neuronal and glial subtypes. Using SLAM-seq, we found that chronic stress led to higher turnover of a group of genes associated with myelination, and this finding is confirmed by immunostaining which showed increased myelination in the chronically stressed amygdala. Additionally, genes detected by SLAM-seq and RNA-seq only partially overlapped, suggesting that SLAM-seq and RNA-seq are complementary in identifying stress-responsive genes. By applying SLAM-seq in vivo, we obtained a rich dataset of genes with higher turnover in the amygdala under stress.http://www.sciencedirect.com/science/article/pii/S2352289524000845SLAM-seqAmygdalaAcute stressChronic stress |
| spellingShingle | Dan Zhao Lu Zhang Yang Yang Transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol-labeled RNA sequencing Neurobiology of Stress SLAM-seq Amygdala Acute stress Chronic stress |
| title | Transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol-labeled RNA sequencing |
| title_full | Transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol-labeled RNA sequencing |
| title_fullStr | Transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol-labeled RNA sequencing |
| title_full_unstemmed | Transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol-labeled RNA sequencing |
| title_short | Transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol-labeled RNA sequencing |
| title_sort | transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol labeled rna sequencing |
| topic | SLAM-seq Amygdala Acute stress Chronic stress |
| url | http://www.sciencedirect.com/science/article/pii/S2352289524000845 |
| work_keys_str_mv | AT danzhao transcriptomedynamicsinmouseamygdalaunderacuteandchronicstressrevealedbythiollabeledrnasequencing AT luzhang transcriptomedynamicsinmouseamygdalaunderacuteandchronicstressrevealedbythiollabeledrnasequencing AT yangyang transcriptomedynamicsinmouseamygdalaunderacuteandchronicstressrevealedbythiollabeledrnasequencing |