Quantitative Proteomic Analysis of Hepatic Tissue of T2DM Rhesus Macaque
Type 2 diabetes mellitus (T2DM) is a metabolic disorder that severely affects human health, but the pathogenesis of the disease remains unknown. The high-fat/high-sucrose diets combined with streptozotocin- (STZ-) induced nonhuman primate animal model of diabetes are a valuable research source of T2...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/3601708 |
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| author | Tingfu Du Shuaiyao Lu Qinfang Jiang Yun Li Kaili Ma |
| author_facet | Tingfu Du Shuaiyao Lu Qinfang Jiang Yun Li Kaili Ma |
| author_sort | Tingfu Du |
| collection | DOAJ |
| description | Type 2 diabetes mellitus (T2DM) is a metabolic disorder that severely affects human health, but the pathogenesis of the disease remains unknown. The high-fat/high-sucrose diets combined with streptozotocin- (STZ-) induced nonhuman primate animal model of diabetes are a valuable research source of T2DM. Here, we present a study of a STZ rhesus macaque model of T2DM that utilizes quantitative iTRAQ-based proteomic method. We compared the protein profiles in the liver of STZ-treated macaques as well as age-matched healthy controls. We identified 171 proteins differentially expressed in the STZ-treated groups, about 70 of which were documented as diabetes-related gene in previous studies. Pathway analyses indicated that the biological functions of differentially expressed proteins were related to glycolysis/gluconeogenesis, fatty acid metabolism, complements, and coagulation cascades. Expression change in tryptophan metabolism pathway was also found in this study which may be associations with diabetes. This study is the first to explore genome-wide protein expression in hepatic tissue of diabetes macaque model using HPLC-Q-TOF/MS technology. In addition to providing potential T2DM biomarkers, this quantitative proteomic study may also shed insights regarding the molecular pathogenesis of T2DM. |
| format | Article |
| id | doaj-art-152e3742fb17491a940c721acc36d042 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-152e3742fb17491a940c721acc36d0422025-02-03T01:20:52ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/36017083601708Quantitative Proteomic Analysis of Hepatic Tissue of T2DM Rhesus MacaqueTingfu Du0Shuaiyao Lu1Qinfang Jiang2Yun Li3Kaili Ma4Center for Drug Safety Evaluation and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, ChinaCenter for Drug Safety Evaluation and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, ChinaCenter for Drug Safety Evaluation and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, ChinaCenter for Drug Safety Evaluation and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, ChinaCenter for Drug Safety Evaluation and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, ChinaType 2 diabetes mellitus (T2DM) is a metabolic disorder that severely affects human health, but the pathogenesis of the disease remains unknown. The high-fat/high-sucrose diets combined with streptozotocin- (STZ-) induced nonhuman primate animal model of diabetes are a valuable research source of T2DM. Here, we present a study of a STZ rhesus macaque model of T2DM that utilizes quantitative iTRAQ-based proteomic method. We compared the protein profiles in the liver of STZ-treated macaques as well as age-matched healthy controls. We identified 171 proteins differentially expressed in the STZ-treated groups, about 70 of which were documented as diabetes-related gene in previous studies. Pathway analyses indicated that the biological functions of differentially expressed proteins were related to glycolysis/gluconeogenesis, fatty acid metabolism, complements, and coagulation cascades. Expression change in tryptophan metabolism pathway was also found in this study which may be associations with diabetes. This study is the first to explore genome-wide protein expression in hepatic tissue of diabetes macaque model using HPLC-Q-TOF/MS technology. In addition to providing potential T2DM biomarkers, this quantitative proteomic study may also shed insights regarding the molecular pathogenesis of T2DM.http://dx.doi.org/10.1155/2017/3601708 |
| spellingShingle | Tingfu Du Shuaiyao Lu Qinfang Jiang Yun Li Kaili Ma Quantitative Proteomic Analysis of Hepatic Tissue of T2DM Rhesus Macaque Journal of Diabetes Research |
| title | Quantitative Proteomic Analysis of Hepatic Tissue of T2DM Rhesus Macaque |
| title_full | Quantitative Proteomic Analysis of Hepatic Tissue of T2DM Rhesus Macaque |
| title_fullStr | Quantitative Proteomic Analysis of Hepatic Tissue of T2DM Rhesus Macaque |
| title_full_unstemmed | Quantitative Proteomic Analysis of Hepatic Tissue of T2DM Rhesus Macaque |
| title_short | Quantitative Proteomic Analysis of Hepatic Tissue of T2DM Rhesus Macaque |
| title_sort | quantitative proteomic analysis of hepatic tissue of t2dm rhesus macaque |
| url | http://dx.doi.org/10.1155/2017/3601708 |
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