The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study
Yam tyrosinase has become an economically essential enzyme due to its ease of purification and abundant availability of yam tubers. However, an efficient biochemical and biophysical characterization of yam tyrosinase has not been reported. In the present study, the interaction of yam (Amorphophallus...
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Wiley
2019-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2019/8284968 |
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| author | Tabassum Mulla Sushama Patil Srinivas Sistla Jyoti Jadhav |
| author_facet | Tabassum Mulla Sushama Patil Srinivas Sistla Jyoti Jadhav |
| author_sort | Tabassum Mulla |
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| description | Yam tyrosinase has become an economically essential enzyme due to its ease of purification and abundant availability of yam tubers. However, an efficient biochemical and biophysical characterization of yam tyrosinase has not been reported. In the present study, the interaction of yam (Amorphophallus paeoniifolius) tyrosinase was studied with molecules such as crocin (Crocus sativus), hydroquinone, and kojic acid. Surface plasmon resonance (SPR), fluorescence spectroscopy, and circular dichroism techniques were employed to determine the binding affinities and the changes in secondary and tertiary structures of yam tyrosinase in the presence of four relevant small molecules. Hydroquinone and crocin exhibited very low binding affinities of 0.24 M and 0.0017 M. Due to their apparent weak interactions, competition experiments were used to determine more precisely the binding affinities. Structure-function interrelationships can be correlated in great detail by this study, and the results can be compared with other available tyrosinases. |
| format | Article |
| id | doaj-art-15031b4290544f32b3b275ae9c8f6027 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
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| series | Biochemistry Research International |
| spelling | doaj-art-15031b4290544f32b3b275ae9c8f60272025-02-03T06:12:23ZengWileyBiochemistry Research International2090-22472090-22552019-01-01201910.1155/2019/82849688284968The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical StudyTabassum Mulla0Sushama Patil1Srinivas Sistla2Jyoti Jadhav3Department of Biotechnology, Shivaji University, Kolhapur 416004, IndiaDepartment of Biotechnology, Shivaji University, Kolhapur 416004, IndiaInstitute for Structural Biology, Drug Discovery and Development, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Biotechnology, Shivaji University, Kolhapur 416004, IndiaYam tyrosinase has become an economically essential enzyme due to its ease of purification and abundant availability of yam tubers. However, an efficient biochemical and biophysical characterization of yam tyrosinase has not been reported. In the present study, the interaction of yam (Amorphophallus paeoniifolius) tyrosinase was studied with molecules such as crocin (Crocus sativus), hydroquinone, and kojic acid. Surface plasmon resonance (SPR), fluorescence spectroscopy, and circular dichroism techniques were employed to determine the binding affinities and the changes in secondary and tertiary structures of yam tyrosinase in the presence of four relevant small molecules. Hydroquinone and crocin exhibited very low binding affinities of 0.24 M and 0.0017 M. Due to their apparent weak interactions, competition experiments were used to determine more precisely the binding affinities. Structure-function interrelationships can be correlated in great detail by this study, and the results can be compared with other available tyrosinases.http://dx.doi.org/10.1155/2019/8284968 |
| spellingShingle | Tabassum Mulla Sushama Patil Srinivas Sistla Jyoti Jadhav The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study Biochemistry Research International |
| title | The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study |
| title_full | The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study |
| title_fullStr | The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study |
| title_full_unstemmed | The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study |
| title_short | The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study |
| title_sort | binding affinity of small molecules with yam tyrosinase catechol oxidase a biophysical study |
| url | http://dx.doi.org/10.1155/2019/8284968 |
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