Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines
B cell specific immunomodulatory drugs still remain an unmet medical need. Utilisation of validated simplified in vitro models would allow readily obtaining new insights in the complexity of B cell regulation. For this purpose we investigated which human B lymphocyte stimulation assays may be ideall...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/5281823 |
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| _version_ | 1832548587424186368 |
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| author | Kristien Van Belle Jean Herman Louis Boon Mark Waer Ben Sprangers Thierry Louat |
| author_facet | Kristien Van Belle Jean Herman Louis Boon Mark Waer Ben Sprangers Thierry Louat |
| author_sort | Kristien Van Belle |
| collection | DOAJ |
| description | B cell specific immunomodulatory drugs still remain an unmet medical need. Utilisation of validated simplified in vitro models would allow readily obtaining new insights in the complexity of B cell regulation. For this purpose we investigated which human B lymphocyte stimulation assays may be ideally suited to investigate new B lymphocyte immunosuppressants. Primary polyclonal human B cells underwent in vitro stimulation and their proliferation, production of immunoglobulins (Igs) and of cytokines, and expression of cell surface molecules were analysed using various stimuli. ODN2006, a toll-like receptor 9 (TLR9) agonist, was the most potent general B cell stimulus. Subsequently, we investigated on which human B cell lines ODN2006 evoked the broadest immunostimulatory effects. The Namalwa cell line proved to be the most responsive upon TLR9 stimulation and hence may serve as a relevant, homogeneous, and stable B cell model in an in vitro phenotypic assay for the discovery of new targets and inhibitors of the B cell activation processes. As for the read-out for such screening assay, it is proposed that the expression of activation and costimulatory surface markers reliably reflects B lymphocyte activation. |
| format | Article |
| id | doaj-art-15016fc0f5464b91bc4cb9b427606dd2 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-15016fc0f5464b91bc4cb9b427606dd22025-02-03T06:13:38ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/52818235281823Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell LinesKristien Van Belle0Jean Herman1Louis Boon2Mark Waer3Ben Sprangers4Thierry Louat5KU Leuven, Interface Valorisation Platform (IVAP), 3000 Leuven, BelgiumKU Leuven, Interface Valorisation Platform (IVAP), 3000 Leuven, BelgiumBioceros, 3584 CM Utrecht, NetherlandsKU Leuven, Interface Valorisation Platform (IVAP), 3000 Leuven, BelgiumKU Leuven, Interface Valorisation Platform (IVAP), 3000 Leuven, BelgiumKU Leuven, Interface Valorisation Platform (IVAP), 3000 Leuven, BelgiumB cell specific immunomodulatory drugs still remain an unmet medical need. Utilisation of validated simplified in vitro models would allow readily obtaining new insights in the complexity of B cell regulation. For this purpose we investigated which human B lymphocyte stimulation assays may be ideally suited to investigate new B lymphocyte immunosuppressants. Primary polyclonal human B cells underwent in vitro stimulation and their proliferation, production of immunoglobulins (Igs) and of cytokines, and expression of cell surface molecules were analysed using various stimuli. ODN2006, a toll-like receptor 9 (TLR9) agonist, was the most potent general B cell stimulus. Subsequently, we investigated on which human B cell lines ODN2006 evoked the broadest immunostimulatory effects. The Namalwa cell line proved to be the most responsive upon TLR9 stimulation and hence may serve as a relevant, homogeneous, and stable B cell model in an in vitro phenotypic assay for the discovery of new targets and inhibitors of the B cell activation processes. As for the read-out for such screening assay, it is proposed that the expression of activation and costimulatory surface markers reliably reflects B lymphocyte activation.http://dx.doi.org/10.1155/2016/5281823 |
| spellingShingle | Kristien Van Belle Jean Herman Louis Boon Mark Waer Ben Sprangers Thierry Louat Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines Journal of Immunology Research |
| title | Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines |
| title_full | Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines |
| title_fullStr | Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines |
| title_full_unstemmed | Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines |
| title_short | Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines |
| title_sort | comparative in vitro immune stimulation analysis of primary human b cells and b cell lines |
| url | http://dx.doi.org/10.1155/2016/5281823 |
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