Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-Denitrifier
Heterotrophic nitrifiers synthesize nitrogenous gasses when nitrifying ammonium ion. A Cupriavidus pauculus, previously thought an Alcaligenes sp. and noted as an active heterotrophic nitrifier-denitrifier, was examined for its ability to produce nitrogen gas (N2) and nitrous oxide (N2O) while heter...
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/901702 |
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| author | Miguel Ramirez Jennifer Obrzydowski Mary Ayers Sonia Virparia Meijing Wang Kurtis Stefan Richard Linchangco Domenic Castignetti |
| author_facet | Miguel Ramirez Jennifer Obrzydowski Mary Ayers Sonia Virparia Meijing Wang Kurtis Stefan Richard Linchangco Domenic Castignetti |
| author_sort | Miguel Ramirez |
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| description | Heterotrophic nitrifiers synthesize nitrogenous gasses when nitrifying ammonium ion. A Cupriavidus pauculus, previously thought an Alcaligenes sp. and noted as an active heterotrophic nitrifier-denitrifier, was examined for its ability to produce nitrogen gas (N2) and nitrous oxide (N2O) while heterotrophically nitrifying the organic substrate pyruvic oxime [CH3–C(NOH)–COOH]. Neither N2 nor N2O were produced. Nucleotide and phylogenetic analyses indicated that the organism is a member of a genus (Cupriavidus) known for its resistance to metals and its metabolism of xenobiotics. The microbe (a Cupriavidus pauculus designated as C. pauculus strain UM1) was examined for its ability to perform heterotrophic nitrification in the presence of Cu2+ and Ni2+ and to metabolize the xenobiotic phenol. The bacterium heterotrophically nitrified well when either 1 mM Cu2+ or 0.5 mM Ni2+ was present in either enriched or minimal medium. The organism also used phenol as a sole carbon source in either the presence or absence of 1 mM Cu2+ or 0.5 mM Ni2+. The ability of this isolate to perform a number of different metabolisms, its noteworthy resistance to copper and nickel, and its potential use as a bioremediation agent are discussed. |
| format | Article |
| id | doaj-art-15016b15c83840968160257e9b200fc3 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-15016b15c83840968160257e9b200fc32025-02-03T01:03:30ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/901702901702Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-DenitrifierMiguel Ramirez0Jennifer Obrzydowski1Mary Ayers2Sonia Virparia3Meijing Wang4Kurtis Stefan5Richard Linchangco6Domenic Castignetti7Department of Biology, Loyola University of Chicago, 1032 West Sheridan Road, Chicago, IL 60626, USADepartment of Biology, Loyola University of Chicago, 1032 West Sheridan Road, Chicago, IL 60626, USADepartment of Biology, Loyola University of Chicago, 1032 West Sheridan Road, Chicago, IL 60626, USADepartment of Biology, Loyola University of Chicago, 1032 West Sheridan Road, Chicago, IL 60626, USADepartment of Biology, Loyola University of Chicago, 1032 West Sheridan Road, Chicago, IL 60626, USADepartment of Biology, Loyola University of Chicago, 1032 West Sheridan Road, Chicago, IL 60626, USADepartment of Biology, Loyola University of Chicago, 1032 West Sheridan Road, Chicago, IL 60626, USADepartment of Biology, Loyola University of Chicago, 1032 West Sheridan Road, Chicago, IL 60626, USAHeterotrophic nitrifiers synthesize nitrogenous gasses when nitrifying ammonium ion. A Cupriavidus pauculus, previously thought an Alcaligenes sp. and noted as an active heterotrophic nitrifier-denitrifier, was examined for its ability to produce nitrogen gas (N2) and nitrous oxide (N2O) while heterotrophically nitrifying the organic substrate pyruvic oxime [CH3–C(NOH)–COOH]. Neither N2 nor N2O were produced. Nucleotide and phylogenetic analyses indicated that the organism is a member of a genus (Cupriavidus) known for its resistance to metals and its metabolism of xenobiotics. The microbe (a Cupriavidus pauculus designated as C. pauculus strain UM1) was examined for its ability to perform heterotrophic nitrification in the presence of Cu2+ and Ni2+ and to metabolize the xenobiotic phenol. The bacterium heterotrophically nitrified well when either 1 mM Cu2+ or 0.5 mM Ni2+ was present in either enriched or minimal medium. The organism also used phenol as a sole carbon source in either the presence or absence of 1 mM Cu2+ or 0.5 mM Ni2+. The ability of this isolate to perform a number of different metabolisms, its noteworthy resistance to copper and nickel, and its potential use as a bioremediation agent are discussed.http://dx.doi.org/10.1155/2014/901702 |
| spellingShingle | Miguel Ramirez Jennifer Obrzydowski Mary Ayers Sonia Virparia Meijing Wang Kurtis Stefan Richard Linchangco Domenic Castignetti Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-Denitrifier The Scientific World Journal |
| title | Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-Denitrifier |
| title_full | Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-Denitrifier |
| title_fullStr | Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-Denitrifier |
| title_full_unstemmed | Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-Denitrifier |
| title_short | Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-Denitrifier |
| title_sort | pyruvic oxime nitrification and copper and nickel resistance by a cupriavidus pauculus an active heterotrophic nitrifier denitrifier |
| url | http://dx.doi.org/10.1155/2014/901702 |
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