Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans
In this study, we explored the effect of two arsenolipids with different molecular masses, AsHC 332 and AsHC 346, which are commonly found in seafood, on aging and lifespan-related indicators in the model organism Caenorhabditis elegans. The possible mechanism by which the arsenolipids promote aging...
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China Food Publishing Company
2024-12-01
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-23-015.pdf |
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| author | YIN Huan, SONG Bingbing, LI Ziyu, WANG Zhuo, ZHAO Qiaoli, LI Rui, CHEN Jianping, ZHONG Saiyi |
| author_facet | YIN Huan, SONG Bingbing, LI Ziyu, WANG Zhuo, ZHAO Qiaoli, LI Rui, CHEN Jianping, ZHONG Saiyi |
| author_sort | YIN Huan, SONG Bingbing, LI Ziyu, WANG Zhuo, ZHAO Qiaoli, LI Rui, CHEN Jianping, ZHONG Saiyi |
| collection | DOAJ |
| description | In this study, we explored the effect of two arsenolipids with different molecular masses, AsHC 332 and AsHC 346, which are commonly found in seafood, on aging and lifespan-related indicators in the model organism Caenorhabditis elegans. The possible mechanism by which the arsenolipids promote aging was analyzed by measuring the expression of DAF-16 protein in the nematode, the longevity of aging mutants and stress-related genes. The results showed that AsHCs significantly reduced the body length, body width, brood size and head thrash frequency of C. elegans in a dose-dependent manner (P < 0.05). Moreover, the resistance to heat stress (P < 0.000 1) and ultraviolet stress (P < 0.05) was significantly reduced by AsHCs. The lifespan of C. elegans was significantly shortened (P < 0.01). The accumulation of lipofuscin was increased (P < 0.001). The relative expression of the daf-16, sod-3, ctl-1, gst-4 and hsp-16.2 genes was up-regulated, while the nuclear localization of DAF-16 was not significantly promoted, and there was no effect on the lifespan of the daf-16(mu86) mutant. AsHC 332 and AsHC 346 had the same toxic effects on nematode aging, which may shorten the lifespan of C. elegans by regulating the transcription factor DAF-16. |
| format | Article |
| id | doaj-art-14f357049f4c40bc9e0a49a960c2b756 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-14f357049f4c40bc9e0a49a960c2b7562025-02-05T09:07:53ZengChina Food Publishing CompanyShipin Kexue1002-66302024-12-01452313113910.7506/spkx1002-6630-20231226-227Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegansYIN Huan, SONG Bingbing, LI Ziyu, WANG Zhuo, ZHAO Qiaoli, LI Rui, CHEN Jianping, ZHONG Saiyi0(1. Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Marine Food, Guangdong Sub-tropical Fruit and Vegetable Processing Technology Innovation Center, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 2. Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, China)In this study, we explored the effect of two arsenolipids with different molecular masses, AsHC 332 and AsHC 346, which are commonly found in seafood, on aging and lifespan-related indicators in the model organism Caenorhabditis elegans. The possible mechanism by which the arsenolipids promote aging was analyzed by measuring the expression of DAF-16 protein in the nematode, the longevity of aging mutants and stress-related genes. The results showed that AsHCs significantly reduced the body length, body width, brood size and head thrash frequency of C. elegans in a dose-dependent manner (P < 0.05). Moreover, the resistance to heat stress (P < 0.000 1) and ultraviolet stress (P < 0.05) was significantly reduced by AsHCs. The lifespan of C. elegans was significantly shortened (P < 0.01). The accumulation of lipofuscin was increased (P < 0.001). The relative expression of the daf-16, sod-3, ctl-1, gst-4 and hsp-16.2 genes was up-regulated, while the nuclear localization of DAF-16 was not significantly promoted, and there was no effect on the lifespan of the daf-16(mu86) mutant. AsHC 332 and AsHC 346 had the same toxic effects on nematode aging, which may shorten the lifespan of C. elegans by regulating the transcription factor DAF-16.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-23-015.pdfarsenolipids; organic arsenic; toxicity; aging; molecular mechanism; caenorhabditis elegans |
| spellingShingle | YIN Huan, SONG Bingbing, LI Ziyu, WANG Zhuo, ZHAO Qiaoli, LI Rui, CHEN Jianping, ZHONG Saiyi Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans Shipin Kexue arsenolipids; organic arsenic; toxicity; aging; molecular mechanism; caenorhabditis elegans |
| title | Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans |
| title_full | Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans |
| title_fullStr | Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans |
| title_full_unstemmed | Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans |
| title_short | Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans |
| title_sort | toxic effect and molecular mechanism of arsenolipids on promoting aging in caenorhabditis elegans |
| topic | arsenolipids; organic arsenic; toxicity; aging; molecular mechanism; caenorhabditis elegans |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-23-015.pdf |
| work_keys_str_mv | AT yinhuansongbingbingliziyuwangzhuozhaoqiaoliliruichenjianpingzhongsaiyi toxiceffectandmolecularmechanismofarsenolipidsonpromotingagingincaenorhabditiselegans |