A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice
Excessive fat liver is an important manifestation of nonalcoholic fatty liver disease (NAFLD), associated with obesity, insulin resistance, and oxidative stress. In the present study, the effects of a high-fat, high-fructose diet (HFFD) on mRNA levels and activities of the antioxidant enzymes, inclu...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2016/5029414 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545693130031104 |
|---|---|
| author | Kanokwan Jarukamjorn Nattharat Jearapong Charinya Pimson Waranya Chatuphonprasert |
| author_facet | Kanokwan Jarukamjorn Nattharat Jearapong Charinya Pimson Waranya Chatuphonprasert |
| author_sort | Kanokwan Jarukamjorn |
| collection | DOAJ |
| description | Excessive fat liver is an important manifestation of nonalcoholic fatty liver disease (NAFLD), associated with obesity, insulin resistance, and oxidative stress. In the present study, the effects of a high-fat, high-fructose diet (HFFD) on mRNA levels and activities of the antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), were determined in mouse livers and brains. The histomorphology of the livers was examined and the state of nonenzymatic reducing system was evaluated by measuring the glutathione system and the lipid peroxidation. Histopathology of the liver showed that fat accumulation and inflammation depended on the period of the HFFD-consumption. The levels of mRNA and enzymatic activities of SOD, CAT, and GPx were raised, followed by the increases in malondialdehyde levels in livers and brains of the HFFD mice. The oxidized GSSG content was increased while the total GSH and the reduced GSH were decreased, resulting in the increase in the GSH/GSSG ratio in both livers and brains of the HFFD mice. These observations suggested that liver damage and oxidative stress in the significant organs were generated by continuous HFFD-consumption. Imbalance of antioxidant condition induced by long-term HFFD-consumption might increase the risk and progression of NAFLD. |
| format | Article |
| id | doaj-art-14f22303bc3446e29a77b38f7733d02e |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-14f22303bc3446e29a77b38f7733d02e2025-02-03T07:24:56ZengWileyScientifica2090-908X2016-01-01201610.1155/2016/50294145029414A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in MiceKanokwan Jarukamjorn0Nattharat Jearapong1Charinya Pimson2Waranya Chatuphonprasert3Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandResearch Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandResearch Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Medicine, Mahasarakham University, Mahasarakham 44000, ThailandExcessive fat liver is an important manifestation of nonalcoholic fatty liver disease (NAFLD), associated with obesity, insulin resistance, and oxidative stress. In the present study, the effects of a high-fat, high-fructose diet (HFFD) on mRNA levels and activities of the antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), were determined in mouse livers and brains. The histomorphology of the livers was examined and the state of nonenzymatic reducing system was evaluated by measuring the glutathione system and the lipid peroxidation. Histopathology of the liver showed that fat accumulation and inflammation depended on the period of the HFFD-consumption. The levels of mRNA and enzymatic activities of SOD, CAT, and GPx were raised, followed by the increases in malondialdehyde levels in livers and brains of the HFFD mice. The oxidized GSSG content was increased while the total GSH and the reduced GSH were decreased, resulting in the increase in the GSH/GSSG ratio in both livers and brains of the HFFD mice. These observations suggested that liver damage and oxidative stress in the significant organs were generated by continuous HFFD-consumption. Imbalance of antioxidant condition induced by long-term HFFD-consumption might increase the risk and progression of NAFLD.http://dx.doi.org/10.1155/2016/5029414 |
| spellingShingle | Kanokwan Jarukamjorn Nattharat Jearapong Charinya Pimson Waranya Chatuphonprasert A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice Scientifica |
| title | A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice |
| title_full | A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice |
| title_fullStr | A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice |
| title_full_unstemmed | A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice |
| title_short | A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice |
| title_sort | high fat high fructose diet induces antioxidant imbalance and increases the risk and progression of nonalcoholic fatty liver disease in mice |
| url | http://dx.doi.org/10.1155/2016/5029414 |
| work_keys_str_mv | AT kanokwanjarukamjorn ahighfathighfructosedietinducesantioxidantimbalanceandincreasestheriskandprogressionofnonalcoholicfattyliverdiseaseinmice AT nattharatjearapong ahighfathighfructosedietinducesantioxidantimbalanceandincreasestheriskandprogressionofnonalcoholicfattyliverdiseaseinmice AT charinyapimson ahighfathighfructosedietinducesantioxidantimbalanceandincreasestheriskandprogressionofnonalcoholicfattyliverdiseaseinmice AT waranyachatuphonprasert ahighfathighfructosedietinducesantioxidantimbalanceandincreasestheriskandprogressionofnonalcoholicfattyliverdiseaseinmice AT kanokwanjarukamjorn highfathighfructosedietinducesantioxidantimbalanceandincreasestheriskandprogressionofnonalcoholicfattyliverdiseaseinmice AT nattharatjearapong highfathighfructosedietinducesantioxidantimbalanceandincreasestheriskandprogressionofnonalcoholicfattyliverdiseaseinmice AT charinyapimson highfathighfructosedietinducesantioxidantimbalanceandincreasestheriskandprogressionofnonalcoholicfattyliverdiseaseinmice AT waranyachatuphonprasert highfathighfructosedietinducesantioxidantimbalanceandincreasestheriskandprogressionofnonalcoholicfattyliverdiseaseinmice |