TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma
Background. Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies and is associated with high mortality. Transmembrane protein 147 (TMEM147) is a seven-transmembrane protein that may mediate immune regulation. However, the relevance of TMEM147 to immune regulation in HCC and the p...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2023/4413049 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546715479048192 |
|---|---|
| author | Sheng Cheng Jutang Li Ming Xu Qun Bao Jiaoxiang Wu Peng Sun Bo Han |
| author_facet | Sheng Cheng Jutang Li Ming Xu Qun Bao Jiaoxiang Wu Peng Sun Bo Han |
| author_sort | Sheng Cheng |
| collection | DOAJ |
| description | Background. Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies and is associated with high mortality. Transmembrane protein 147 (TMEM147) is a seven-transmembrane protein that may mediate immune regulation. However, the relevance of TMEM147 to immune regulation in HCC and the prognosis of HCC patients are unclear. Methods. We analyzed TMEM147 expression in HCC by using the Wilcoxon rank-sum test. Real time quantitative PCR (RT-qPCR) and Western blot analysis of tumor tissues and cell lines were used to verify TMEM147 expression in HCC. The influence of TMEM147 on HCC prognosis was assessed using Kaplan–Meier analysis, Cox regression analysis, and a prognostic nomogram. The functions of the TMEM147-related differentially expressed genes (DEGs) were identified by Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and gene set enrichment analysis (GSEA). In addition, we examined the associations between TMEM147 expression and immune infiltration using single-sample gene set enrichment analysis (ssGSEA) and immunofluorescence staining of HCC tissues. Results. Our results showed that the expression of TMEM147 was significantly higher in human HCC tissues than in adjacent normal liver tissues, with similar findings in human HCC cell lines. High TMEM147 expression was correlated with T stage, pathological stage, histological grade, race, alpha-fetoprotein level, and vascular invasion in HCC. Moreover, we revealed that high TMEM147 expression was associated with shorter survival times and that TMEM147 could be a risk factor for overall survival, along with T stage, M stage, pathological stage, and tumor status. Mechanistic studies revealed that high TMEM147 expression was linked to the B lymphocyte, antigen response, IL6 signaling pathway, cell cycle, Kirsten rat sarcoma viral oncogene homolog (KRAS) signaling pathway, and myelocytomatosis oncogene (MYC) targets. Correspondingly, TMEM147 expression was positively associated with the infiltration of immune cells, including Th2 cells, follicular helper T cells, macrophages, and NK CD56 bright cells in HCC. Conclusions. TMEM147 might be a biomarker for poor prognosis and is related to immune cell infiltration in HCC. |
| format | Article |
| id | doaj-art-14e4820ed21f44babede3b135b82c68a |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-14e4820ed21f44babede3b135b82c68a2025-02-03T06:47:22ZengWileyAnalytical Cellular Pathology2210-71852023-01-01202310.1155/2023/4413049TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular CarcinomaSheng Cheng0Jutang Li1Ming Xu2Qun Bao3Jiaoxiang Wu4Peng Sun5Bo Han6Department of General SurgeryKey Laboratory for Translational Research and Innovative Therapeutics of Gastrointestinal OncologyDepartment of General SurgeryDepartment of General SurgeryKey Laboratory for Translational Research and Innovative Therapeutics of Gastrointestinal OncologyDepartment of General SurgeryDepartment of General SurgeryBackground. Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies and is associated with high mortality. Transmembrane protein 147 (TMEM147) is a seven-transmembrane protein that may mediate immune regulation. However, the relevance of TMEM147 to immune regulation in HCC and the prognosis of HCC patients are unclear. Methods. We analyzed TMEM147 expression in HCC by using the Wilcoxon rank-sum test. Real time quantitative PCR (RT-qPCR) and Western blot analysis of tumor tissues and cell lines were used to verify TMEM147 expression in HCC. The influence of TMEM147 on HCC prognosis was assessed using Kaplan–Meier analysis, Cox regression analysis, and a prognostic nomogram. The functions of the TMEM147-related differentially expressed genes (DEGs) were identified by Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and gene set enrichment analysis (GSEA). In addition, we examined the associations between TMEM147 expression and immune infiltration using single-sample gene set enrichment analysis (ssGSEA) and immunofluorescence staining of HCC tissues. Results. Our results showed that the expression of TMEM147 was significantly higher in human HCC tissues than in adjacent normal liver tissues, with similar findings in human HCC cell lines. High TMEM147 expression was correlated with T stage, pathological stage, histological grade, race, alpha-fetoprotein level, and vascular invasion in HCC. Moreover, we revealed that high TMEM147 expression was associated with shorter survival times and that TMEM147 could be a risk factor for overall survival, along with T stage, M stage, pathological stage, and tumor status. Mechanistic studies revealed that high TMEM147 expression was linked to the B lymphocyte, antigen response, IL6 signaling pathway, cell cycle, Kirsten rat sarcoma viral oncogene homolog (KRAS) signaling pathway, and myelocytomatosis oncogene (MYC) targets. Correspondingly, TMEM147 expression was positively associated with the infiltration of immune cells, including Th2 cells, follicular helper T cells, macrophages, and NK CD56 bright cells in HCC. Conclusions. TMEM147 might be a biomarker for poor prognosis and is related to immune cell infiltration in HCC.http://dx.doi.org/10.1155/2023/4413049 |
| spellingShingle | Sheng Cheng Jutang Li Ming Xu Qun Bao Jiaoxiang Wu Peng Sun Bo Han TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma Analytical Cellular Pathology |
| title | TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma |
| title_full | TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma |
| title_fullStr | TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma |
| title_full_unstemmed | TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma |
| title_short | TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma |
| title_sort | tmem147 correlates with immune infiltration and serve as a potential prognostic biomarker in hepatocellular carcinoma |
| url | http://dx.doi.org/10.1155/2023/4413049 |
| work_keys_str_mv | AT shengcheng tmem147correlateswithimmuneinfiltrationandserveasapotentialprognosticbiomarkerinhepatocellularcarcinoma AT jutangli tmem147correlateswithimmuneinfiltrationandserveasapotentialprognosticbiomarkerinhepatocellularcarcinoma AT mingxu tmem147correlateswithimmuneinfiltrationandserveasapotentialprognosticbiomarkerinhepatocellularcarcinoma AT qunbao tmem147correlateswithimmuneinfiltrationandserveasapotentialprognosticbiomarkerinhepatocellularcarcinoma AT jiaoxiangwu tmem147correlateswithimmuneinfiltrationandserveasapotentialprognosticbiomarkerinhepatocellularcarcinoma AT pengsun tmem147correlateswithimmuneinfiltrationandserveasapotentialprognosticbiomarkerinhepatocellularcarcinoma AT bohan tmem147correlateswithimmuneinfiltrationandserveasapotentialprognosticbiomarkerinhepatocellularcarcinoma |