Sprayable solutions containing sticky rice oil droplets reduce western flower thrips damage and induce changes in Chrysanthemum leaf chemistry
Thrips are one of the most challenging pests in agricultural crops, including Chrysanthemum. In this study we tested via two plant assays whether solutions containing sticky rice germ oil (RGO) droplets could effectively trap thrips and lower thrips damage on Chrysanthemum. In the first assay, we ad...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1509126/full |
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| author | Thijs V. Bierman Hocelayne P. Fernandes Hocelayne P. Fernandes Young H. Choi Sumin Seo Klaas Vrieling Mirka Macel Bram Knegt Thomas E. Kodger Ralph van Zwieten Peter G. L. Klinkhamer T. Martijn Bezemer |
| author_facet | Thijs V. Bierman Hocelayne P. Fernandes Hocelayne P. Fernandes Young H. Choi Sumin Seo Klaas Vrieling Mirka Macel Bram Knegt Thomas E. Kodger Ralph van Zwieten Peter G. L. Klinkhamer T. Martijn Bezemer |
| author_sort | Thijs V. Bierman |
| collection | DOAJ |
| description | Thrips are one of the most challenging pests in agricultural crops, including Chrysanthemum. In this study we tested via two plant assays whether solutions containing sticky rice germ oil (RGO) droplets could effectively trap thrips and lower thrips damage on Chrysanthemum. In the first assay, we additionally assessed the metabolomic effects of these RGO droplet sprays and thrips presence on plant chemistry via 1H NMR and headspace GC-MS on multiple timepoints to investigate which plant metabolites were affected by spraying and their potential relation to plant resistance against thrips. In the second assay, we tested the individual RGO solution constituents against thrips. Our results suggested that the adhesive RGO droplets were not effective as a physical trap as only three out of 600 adult thrips were caught at the achieved coverage. However, average thrips damage was still reduced up to 50% and no negative effects on plant growth were observed up to 25 days. Results from the second plant assay indicated that the individual constituents of the solution containing RGO droplets may have direct effects against thrips. Metabolomics analysis of sprayed leaves via headspace GC-MS and 1H NMR indicated that fatty acids and several volatile compounds such as 4(10)-thujene (sabinene), eucalyptol, cis-4-thujanol, and isocaryophyllene were highest on day 10, while sucrose, malic acid, o-Cymene, and 3-Methyl-2-butenoic acid were highest on day 25. Plants with thrips showed higher flavonoid, carbohydrate and glutamine acetic acid levels, and lower fatty acids and malic acid levels. RGO application increased the levels of fatty acids and alcohols present on top of and inside the Chrysanthemum leaves, while decreasing the concentrations of volatile compounds such as eucalyptol, chrysanthenone and eugenol in the Chrysanthemum leaves. Most interestingly, the thrips effect on the plant metabolome was no longer visible in RGO treated plants at the later harvesttime, suggesting that RGO application may overrule or prevent the metabolomic effects of thrips infestation. In conclusion, our study provides new information on how the application of a new plant-based plant protection product affects insect herbivores and alters crop phytochemistry for improved herbivore resistance. |
| format | Article |
| id | doaj-art-1486a765894943a198e7499fb17a8239 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-1486a765894943a198e7499fb17a82392025-01-28T08:33:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011610.3389/fpls.2025.15091261509126Sprayable solutions containing sticky rice oil droplets reduce western flower thrips damage and induce changes in Chrysanthemum leaf chemistryThijs V. Bierman0Hocelayne P. Fernandes1Hocelayne P. Fernandes2Young H. Choi3Sumin Seo4Klaas Vrieling5Mirka Macel6Bram Knegt7Thomas E. Kodger8Ralph van Zwieten9Peter G. L. Klinkhamer10T. Martijn Bezemer11Above-Belowground Interactions, Institute of Biology Leiden, Leiden, NetherlandsAbove-Belowground Interactions, Institute of Biology Leiden, Leiden, NetherlandsNatural Products Laboratory, Institute of Biology Leiden, Leiden, NetherlandsNatural Products Laboratory, Institute of Biology Leiden, Leiden, NetherlandsNatural Products Laboratory, Institute of Biology Leiden, Leiden, NetherlandsAbove-Belowground Interactions, Institute of Biology Leiden, Leiden, NetherlandsWeerbare Planten, Aeres University of Applied Sciences, Almere, NetherlandsWeerbare Planten, Aeres University of Applied Sciences, Almere, NetherlandsPhysical Chemistry and Soft Matter, Agrotechnology & Food Sciences Group, Wageningen University & Research, Wageningen, NetherlandsPhysical Chemistry and Soft Matter, Agrotechnology & Food Sciences Group, Wageningen University & Research, Wageningen, NetherlandsNatural Products Laboratory, Institute of Biology Leiden, Leiden, NetherlandsAbove-Belowground Interactions, Institute of Biology Leiden, Leiden, NetherlandsThrips are one of the most challenging pests in agricultural crops, including Chrysanthemum. In this study we tested via two plant assays whether solutions containing sticky rice germ oil (RGO) droplets could effectively trap thrips and lower thrips damage on Chrysanthemum. In the first assay, we additionally assessed the metabolomic effects of these RGO droplet sprays and thrips presence on plant chemistry via 1H NMR and headspace GC-MS on multiple timepoints to investigate which plant metabolites were affected by spraying and their potential relation to plant resistance against thrips. In the second assay, we tested the individual RGO solution constituents against thrips. Our results suggested that the adhesive RGO droplets were not effective as a physical trap as only three out of 600 adult thrips were caught at the achieved coverage. However, average thrips damage was still reduced up to 50% and no negative effects on plant growth were observed up to 25 days. Results from the second plant assay indicated that the individual constituents of the solution containing RGO droplets may have direct effects against thrips. Metabolomics analysis of sprayed leaves via headspace GC-MS and 1H NMR indicated that fatty acids and several volatile compounds such as 4(10)-thujene (sabinene), eucalyptol, cis-4-thujanol, and isocaryophyllene were highest on day 10, while sucrose, malic acid, o-Cymene, and 3-Methyl-2-butenoic acid were highest on day 25. Plants with thrips showed higher flavonoid, carbohydrate and glutamine acetic acid levels, and lower fatty acids and malic acid levels. RGO application increased the levels of fatty acids and alcohols present on top of and inside the Chrysanthemum leaves, while decreasing the concentrations of volatile compounds such as eucalyptol, chrysanthenone and eugenol in the Chrysanthemum leaves. Most interestingly, the thrips effect on the plant metabolome was no longer visible in RGO treated plants at the later harvesttime, suggesting that RGO application may overrule or prevent the metabolomic effects of thrips infestation. In conclusion, our study provides new information on how the application of a new plant-based plant protection product affects insect herbivores and alters crop phytochemistry for improved herbivore resistance.https://www.frontiersin.org/articles/10.3389/fpls.2025.1509126/fullthripsChrysanthemumintegrated pest managementrice oilmetabolomicsGC-MS |
| spellingShingle | Thijs V. Bierman Hocelayne P. Fernandes Hocelayne P. Fernandes Young H. Choi Sumin Seo Klaas Vrieling Mirka Macel Bram Knegt Thomas E. Kodger Ralph van Zwieten Peter G. L. Klinkhamer T. Martijn Bezemer Sprayable solutions containing sticky rice oil droplets reduce western flower thrips damage and induce changes in Chrysanthemum leaf chemistry Frontiers in Plant Science thrips Chrysanthemum integrated pest management rice oil metabolomics GC-MS |
| title | Sprayable solutions containing sticky rice oil droplets reduce western flower thrips damage and induce changes in Chrysanthemum leaf chemistry |
| title_full | Sprayable solutions containing sticky rice oil droplets reduce western flower thrips damage and induce changes in Chrysanthemum leaf chemistry |
| title_fullStr | Sprayable solutions containing sticky rice oil droplets reduce western flower thrips damage and induce changes in Chrysanthemum leaf chemistry |
| title_full_unstemmed | Sprayable solutions containing sticky rice oil droplets reduce western flower thrips damage and induce changes in Chrysanthemum leaf chemistry |
| title_short | Sprayable solutions containing sticky rice oil droplets reduce western flower thrips damage and induce changes in Chrysanthemum leaf chemistry |
| title_sort | sprayable solutions containing sticky rice oil droplets reduce western flower thrips damage and induce changes in chrysanthemum leaf chemistry |
| topic | thrips Chrysanthemum integrated pest management rice oil metabolomics GC-MS |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1509126/full |
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