Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen
Objective. To explore the molecular mechanism by which oral S2-Ag85DNA vaccines present intestinal antigens. The oral S2-Ag85 vaccine has been shown to protect the human body and effectively improve the titration of the vaccine by acting on intestinal mucosa cells and enhancing their immunogenicity....
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/7200379 |
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| author | Dang Sheng Sui Xin Wu Dongxing Wen Shubo Song Yang Chen Zeliang Zhai Jingbo |
| author_facet | Dang Sheng Sui Xin Wu Dongxing Wen Shubo Song Yang Chen Zeliang Zhai Jingbo |
| author_sort | Dang Sheng |
| collection | DOAJ |
| description | Objective. To explore the molecular mechanism by which oral S2-Ag85DNA vaccines present intestinal antigens. The oral S2-Ag85 vaccine has been shown to protect the human body and effectively improve the titration of the vaccine by acting on intestinal mucosa cells and enhancing their immunogenicity. Method. Mice were immunized with the recombinant S2-Ag85 vaccine, and antibody secretion was then detected in the intestinal tissue. The molecular mechanisms of in vitro detection sensor molecules RIG-1, Pol III, and related conductor transductor molecules DAI, STING, AIM2, IRF3, and IRF7 were determined by separating intestinal IEC, DC, and IELC cells. Results. The S2-Ag85A vaccine was effective in activating dsDNA and RNA transduction pathways in intestinal cells and improving intestinal antigen presentation in mice. |
| format | Article |
| id | doaj-art-148691c2a49c43faa034e86e5a544d49 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-148691c2a49c43faa034e86e5a544d492025-02-03T01:32:33ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/7200379Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal AntigenDang Sheng0Sui Xin1Wu Dongxing2Wen Shubo3Song Yang4Chen Zeliang5Zhai Jingbo6Innovative Institute of ZoonosesInnovative Institute of ZoonosesInnovative Institute of ZoonosesInnovative Institute of ZoonosesInnovative Institute of ZoonosesInnovative Institute of ZoonosesInnovative Institute of ZoonosesObjective. To explore the molecular mechanism by which oral S2-Ag85DNA vaccines present intestinal antigens. The oral S2-Ag85 vaccine has been shown to protect the human body and effectively improve the titration of the vaccine by acting on intestinal mucosa cells and enhancing their immunogenicity. Method. Mice were immunized with the recombinant S2-Ag85 vaccine, and antibody secretion was then detected in the intestinal tissue. The molecular mechanisms of in vitro detection sensor molecules RIG-1, Pol III, and related conductor transductor molecules DAI, STING, AIM2, IRF3, and IRF7 were determined by separating intestinal IEC, DC, and IELC cells. Results. The S2-Ag85A vaccine was effective in activating dsDNA and RNA transduction pathways in intestinal cells and improving intestinal antigen presentation in mice.http://dx.doi.org/10.1155/2022/7200379 |
| spellingShingle | Dang Sheng Sui Xin Wu Dongxing Wen Shubo Song Yang Chen Zeliang Zhai Jingbo Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen Journal of Immunology Research |
| title | Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen |
| title_full | Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen |
| title_fullStr | Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen |
| title_full_unstemmed | Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen |
| title_short | Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen |
| title_sort | oral s2 ag85 dna vaccine activated intestinal cell dsdna and rna sensors to promote the presentation of intestinal antigen |
| url | http://dx.doi.org/10.1155/2022/7200379 |
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