Valorization of Spent Coffee Grounds as a Substrate for Fungal Laccase Production and Biosorbents for Textile Dye Decolorization
Spent coffee grounds (SCG) are a widely available agro-industrial residue rich in carbon and phenolic compounds, presenting significant potential for biotechnological valorization. This study evaluated the use of SCG as a suitable substrate for fungal laccase production and the application of the re...
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MDPI AG
2025-07-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/11/7/396 |
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| author | Eduardo da Silva França Adriana Ferreira de Souza Dayana Montero Rodríguez Nazareth Zimiani de Paula Anna Gabrielly Duarte Neves Kethylen Barbara Barbosa Cardoso Galba Maria de Campos-Takaki Marcos Antonio Barbosa de Lima Ana Lucia Figueiredo Porto |
| author_facet | Eduardo da Silva França Adriana Ferreira de Souza Dayana Montero Rodríguez Nazareth Zimiani de Paula Anna Gabrielly Duarte Neves Kethylen Barbara Barbosa Cardoso Galba Maria de Campos-Takaki Marcos Antonio Barbosa de Lima Ana Lucia Figueiredo Porto |
| author_sort | Eduardo da Silva França |
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| description | Spent coffee grounds (SCG) are a widely available agro-industrial residue rich in carbon and phenolic compounds, presenting significant potential for biotechnological valorization. This study evaluated the use of SCG as a suitable substrate for fungal laccase production and the application of the resulting fermented biomass (RFB), a mixture of fermented SCG and fungal biomass as a biosorbent for textile dye removal. Two fungal strains, namely <i>Lentinus crinitus</i> UCP 1206 and <i>Trametes</i> sp. UCP 1244, were evaluated in both submerged (SmF) and solid-state fermentation (SSF) using SCG. <i>L. crinitus</i> showed superior performance in SSF, reaching 14.62 U/g of laccase activity. Factorial design revealed that a lower SCG amount (5 g) and higher moisture (80%) and temperature (30 °C ± 0.2) favored enzyme production. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) analyses confirmed significant structural degradation of SCG after fermentation, especially in SSF. Furthermore, SCG and RFB were chemically activated and evaluated as biosorbents. The activated carbon from SCG (ACSCG) and RFB (ACRFB) exhibited high removal efficiencies for Remazol dyes, comparable to commercial activated carbon. These findings highlight the potential of SCG as a low-cost, sustainable resource for enzyme production and wastewater treatment, contributing to circular bioeconomy strategies. |
| format | Article |
| id | doaj-art-145cdec0078347928dba1ac8d3e0bd77 |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Fermentation |
| spelling | doaj-art-145cdec0078347928dba1ac8d3e0bd772025-08-20T03:32:12ZengMDPI AGFermentation2311-56372025-07-0111739610.3390/fermentation11070396Valorization of Spent Coffee Grounds as a Substrate for Fungal Laccase Production and Biosorbents for Textile Dye DecolorizationEduardo da Silva França0Adriana Ferreira de Souza1Dayana Montero Rodríguez2Nazareth Zimiani de Paula3Anna Gabrielly Duarte Neves4Kethylen Barbara Barbosa Cardoso5Galba Maria de Campos-Takaki6Marcos Antonio Barbosa de Lima7Ana Lucia Figueiredo Porto8Postgraduate Program in Biotechnology–Northeast Biotechnology Network, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilMultiuser Center for Analysis and Characterization of Biomolecules and Surface Materials, Catholic University of Pernambuco, Recife 50050-900, PE, BrazilMultiuser Center for Analysis and Characterization of Biomolecules and Surface Materials, Catholic University of Pernambuco, Recife 50050-900, PE, BrazilAgricultural and Environmental Microbiology Laboratory, Department of Biology, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilLaboratory of Advances in Protein Biotechnology and Bioactive Technology, Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilBiology Applied to Health Program, Federal University of Pernambuco, Recife 50670-901, PE, BrazilMultiuser Center for Analysis and Characterization of Biomolecules and Surface Materials, Catholic University of Pernambuco, Recife 50050-900, PE, BrazilAgricultural and Environmental Microbiology Laboratory, Department of Biology, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilLaboratory of Advances in Protein Biotechnology and Bioactive Technology, Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilSpent coffee grounds (SCG) are a widely available agro-industrial residue rich in carbon and phenolic compounds, presenting significant potential for biotechnological valorization. This study evaluated the use of SCG as a suitable substrate for fungal laccase production and the application of the resulting fermented biomass (RFB), a mixture of fermented SCG and fungal biomass as a biosorbent for textile dye removal. Two fungal strains, namely <i>Lentinus crinitus</i> UCP 1206 and <i>Trametes</i> sp. UCP 1244, were evaluated in both submerged (SmF) and solid-state fermentation (SSF) using SCG. <i>L. crinitus</i> showed superior performance in SSF, reaching 14.62 U/g of laccase activity. Factorial design revealed that a lower SCG amount (5 g) and higher moisture (80%) and temperature (30 °C ± 0.2) favored enzyme production. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) analyses confirmed significant structural degradation of SCG after fermentation, especially in SSF. Furthermore, SCG and RFB were chemically activated and evaluated as biosorbents. The activated carbon from SCG (ACSCG) and RFB (ACRFB) exhibited high removal efficiencies for Remazol dyes, comparable to commercial activated carbon. These findings highlight the potential of SCG as a low-cost, sustainable resource for enzyme production and wastewater treatment, contributing to circular bioeconomy strategies.https://www.mdpi.com/2311-5637/11/7/396coffee production wastelaccasesolid-state fermentationactivated carbonRemazol dyescircular bioeconomy |
| spellingShingle | Eduardo da Silva França Adriana Ferreira de Souza Dayana Montero Rodríguez Nazareth Zimiani de Paula Anna Gabrielly Duarte Neves Kethylen Barbara Barbosa Cardoso Galba Maria de Campos-Takaki Marcos Antonio Barbosa de Lima Ana Lucia Figueiredo Porto Valorization of Spent Coffee Grounds as a Substrate for Fungal Laccase Production and Biosorbents for Textile Dye Decolorization Fermentation coffee production waste laccase solid-state fermentation activated carbon Remazol dyes circular bioeconomy |
| title | Valorization of Spent Coffee Grounds as a Substrate for Fungal Laccase Production and Biosorbents for Textile Dye Decolorization |
| title_full | Valorization of Spent Coffee Grounds as a Substrate for Fungal Laccase Production and Biosorbents for Textile Dye Decolorization |
| title_fullStr | Valorization of Spent Coffee Grounds as a Substrate for Fungal Laccase Production and Biosorbents for Textile Dye Decolorization |
| title_full_unstemmed | Valorization of Spent Coffee Grounds as a Substrate for Fungal Laccase Production and Biosorbents for Textile Dye Decolorization |
| title_short | Valorization of Spent Coffee Grounds as a Substrate for Fungal Laccase Production and Biosorbents for Textile Dye Decolorization |
| title_sort | valorization of spent coffee grounds as a substrate for fungal laccase production and biosorbents for textile dye decolorization |
| topic | coffee production waste laccase solid-state fermentation activated carbon Remazol dyes circular bioeconomy |
| url | https://www.mdpi.com/2311-5637/11/7/396 |
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