Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells
Compelling evidence from basic molecular biology has demonstrated the crucial role of microglia in the pathogenesis of Alzheimer’s disease (AD). Microglia were believed to play a dual role in both promoting and inhibiting Alzheimer’s disease progression. It is of great significance to regulate the f...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2020/8888871 |
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| author | Tongya Yu Hui Quan Yuzhen Xu Yunxiao Dou Feihong Wang Yingying Lin Xue Qi Yanxin Zhao Xueyuan Liu |
| author_facet | Tongya Yu Hui Quan Yuzhen Xu Yunxiao Dou Feihong Wang Yingying Lin Xue Qi Yanxin Zhao Xueyuan Liu |
| author_sort | Tongya Yu |
| collection | DOAJ |
| description | Compelling evidence from basic molecular biology has demonstrated the crucial role of microglia in the pathogenesis of Alzheimer’s disease (AD). Microglia were believed to play a dual role in both promoting and inhibiting Alzheimer’s disease progression. It is of great significance to regulate the function of microglia and make them develop in a favorable way. In the present study, we investigated the function of repressor element 1-silencing transcription factor (REST) in Aβ1-42-induced BV-2 cell dysfunction. We concluded that Aβ1-42 could promote type I activation of BV-2 cells and induce cell proliferation, migration, and proinflammation cytokine TNF-α, IL-1β, and IL-6 expression. Meanwhile, REST was upregulated, and nuclear translocalization took place due to Aβ1-42 stimulation. When REST was knocked down by a specific short hairpin RNA (sh-RNA), BV-2 cell proliferation, migration, and proinflammation cytokine expression and secretion induced by Aβ1-42 were increased, demonstrating that REST may act as a repressor of microglia-like BV-2 cell activation. |
| format | Article |
| id | doaj-art-144efb0e46544ffc86fd39afbe4abc90 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-144efb0e46544ffc86fd39afbe4abc902025-02-03T01:05:22ZengWileyNeural Plasticity2090-59041687-54432020-01-01202010.1155/2020/88888718888871Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 CellsTongya Yu0Hui Quan1Yuzhen Xu2Yunxiao Dou3Feihong Wang4Yingying Lin5Xue Qi6Yanxin Zhao7Xueyuan Liu8Shanghai Tenth People’s Hospital of Tongji University, Tongji University, Middle Yanchang Rd. 301#, Jingan District, Shanghai, 200072, ChinaShanghai Tenth People’s Hospital of Tongji University, Tongji University, Middle Yanchang Rd. 301#, Jingan District, Shanghai, 200072, ChinaShanghai Tenth People’s Hospital of Tongji University, Tongji University, Middle Yanchang Rd. 301#, Jingan District, Shanghai, 200072, ChinaShanghai Tenth People’s Hospital of Tongji University, Tongji University, Middle Yanchang Rd. 301#, Jingan District, Shanghai, 200072, ChinaShanghai Tenth People’s Hospital of Tongji University, Nanjing Medical University, Middle Yanchang Rd. 301#, Jingan District, Shanghai, 200072, ChinaShanghai Tenth People’s Hospital of Tongji University, Tongji University, Middle Yanchang Rd. 301#, Jingan District, Shanghai, 200072, ChinaShanghai Tenth People’s Hospital of Tongji University, Tongji University, Middle Yanchang Rd. 301#, Jingan District, Shanghai, 200072, ChinaShanghai Tenth People’s Hospital of Tongji University, Tongji University, Middle Yanchang Rd. 301#, Jingan District, Shanghai, 200072, ChinaShanghai Tenth People’s Hospital of Tongji University, Tongji University, Middle Yanchang Rd. 301#, Jingan District, Shanghai, 200072, ChinaCompelling evidence from basic molecular biology has demonstrated the crucial role of microglia in the pathogenesis of Alzheimer’s disease (AD). Microglia were believed to play a dual role in both promoting and inhibiting Alzheimer’s disease progression. It is of great significance to regulate the function of microglia and make them develop in a favorable way. In the present study, we investigated the function of repressor element 1-silencing transcription factor (REST) in Aβ1-42-induced BV-2 cell dysfunction. We concluded that Aβ1-42 could promote type I activation of BV-2 cells and induce cell proliferation, migration, and proinflammation cytokine TNF-α, IL-1β, and IL-6 expression. Meanwhile, REST was upregulated, and nuclear translocalization took place due to Aβ1-42 stimulation. When REST was knocked down by a specific short hairpin RNA (sh-RNA), BV-2 cell proliferation, migration, and proinflammation cytokine expression and secretion induced by Aβ1-42 were increased, demonstrating that REST may act as a repressor of microglia-like BV-2 cell activation.http://dx.doi.org/10.1155/2020/8888871 |
| spellingShingle | Tongya Yu Hui Quan Yuzhen Xu Yunxiao Dou Feihong Wang Yingying Lin Xue Qi Yanxin Zhao Xueyuan Liu Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells Neural Plasticity |
| title | Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells |
| title_full | Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells |
| title_fullStr | Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells |
| title_full_unstemmed | Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells |
| title_short | Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells |
| title_sort | aβ induced repressor element 1 silencing transcription factor rest gene delivery suppresses activation of microglia like bv 2 cells |
| url | http://dx.doi.org/10.1155/2020/8888871 |
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