Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosa
IntroductionPhosphorus (P) is a crucial growth-limiting nutrient in soil, much of which remains challenging for plants to absorb and use. Unlike chemical phosphate fertilizers, phosphate-solubilizing microorganisms (PSMs) offer a means to address available phosphorus deficiency without causing envir...
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Frontiers Media S.A.
2025-02-01
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| author | Yulan Chen Akasha Farooq XieLuyao Wei Leitao Qin Yong Wang Lingzi Zhang Quanju Xiang Ke Zhao Xiumei Yu Qiang Chen Xue Gao Tashi Nyima Petri Penttinen Yunfu Gu |
| author_facet | Yulan Chen Akasha Farooq XieLuyao Wei Leitao Qin Yong Wang Lingzi Zhang Quanju Xiang Ke Zhao Xiumei Yu Qiang Chen Xue Gao Tashi Nyima Petri Penttinen Yunfu Gu |
| author_sort | Yulan Chen |
| collection | DOAJ |
| description | IntroductionPhosphorus (P) is a crucial growth-limiting nutrient in soil, much of which remains challenging for plants to absorb and use. Unlike chemical phosphate fertilizers, phosphate-solubilizing microorganisms (PSMs) offer a means to address available phosphorus deficiency without causing environmental harm. PSMs possess multiple mechanisms for phosphorus solubilization. Although the phosphorus-solubilizing mechanisms of phosphate-solubilizing bacteria (PSB) have been well characterized, the mechanisms utilized by phosphate-solubilizing fungi (PSF) remain largely unexplored.MethodsThis study isolated a PSF strain, Trametes gibbosa T-41, from soil and evaluated its phosphorus solubilizing capacity with organic (calcium phytin; Phytin-P) and inorganic (tricalcium phosphate; Ca-P) phosphorus sources. The phosphorus solubilization, enzyme activity, and organic acid production of T-41 were measured. And the P-solubilizing mechanism conducted by transcriptomic and metabolomic analyses.Results and discussionT-41 exhibited varying phosphorus solubilizing capacity when grown with organic (calcium phytin; Phytin-P) and inorganic (tricalcium phosphate; Ca-P) phosphorus sources (109.80 ± 8.9 mg/L vs. 57.5 ± 7.9 mg/L, p < 0.05). Compared with the Ca-P treatment, T-41 demonstrated a stronger alkaline phosphatase (ALP) production capacity under Phytin-P treatment (34.5 ± 1.2 μmol/L/h vs. 19.8 ± 0.8 μmol/L/h, p < 0.05). Meanwhile, the production of oxalic acid, maleic acid, and succinic acid was higher under Phytin-P treatment (p < 0.05). Transcriptomic and metabolomic analysis revealed that different phosphorus sources altered metabolic pathways such as galactose metabolism, glyoxylate and dicarboxylic acid metabolism, and ascorbate and aldolate metabolism. Key metabolites like myo-inositol, 2-oxoglutarate, and pyruvate were found to impact the performance of T. gibbosa T-41 differently under the two P sources. Notably, synthesis in Ca-P vs. Pytin-P, T-41 upregulated genes involved in myo-inositol synthesis, potentially enhancing its P-solubilizing ability. These results provide new insights into the molecular mechanisms of PSF at the transcriptomic and metabolomic levels, laying a theoretical foundation for the broader application of PSF as bio-phosphorus fertilizers in the future. |
| format | Article |
| id | doaj-art-144c4d9d06b0437db79050ecee2dcf84 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-02-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-144c4d9d06b0437db79050ecee2dcf842025-02-04T06:32:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15204591520459Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosaYulan Chen0Akasha Farooq1XieLuyao Wei2Leitao Qin3Yong Wang4Lingzi Zhang5Quanju Xiang6Ke Zhao7Xiumei Yu8Qiang Chen9Xue Gao10Tashi Nyima11Petri Penttinen12Yunfu Gu13Department of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaInstitute of Agricultural Resources and Environmental Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet, ChinaInstitute of Agricultural Resources and Environmental Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Sources, Sichuan Agricultural University, Chengdu, ChinaIntroductionPhosphorus (P) is a crucial growth-limiting nutrient in soil, much of which remains challenging for plants to absorb and use. Unlike chemical phosphate fertilizers, phosphate-solubilizing microorganisms (PSMs) offer a means to address available phosphorus deficiency without causing environmental harm. PSMs possess multiple mechanisms for phosphorus solubilization. Although the phosphorus-solubilizing mechanisms of phosphate-solubilizing bacteria (PSB) have been well characterized, the mechanisms utilized by phosphate-solubilizing fungi (PSF) remain largely unexplored.MethodsThis study isolated a PSF strain, Trametes gibbosa T-41, from soil and evaluated its phosphorus solubilizing capacity with organic (calcium phytin; Phytin-P) and inorganic (tricalcium phosphate; Ca-P) phosphorus sources. The phosphorus solubilization, enzyme activity, and organic acid production of T-41 were measured. And the P-solubilizing mechanism conducted by transcriptomic and metabolomic analyses.Results and discussionT-41 exhibited varying phosphorus solubilizing capacity when grown with organic (calcium phytin; Phytin-P) and inorganic (tricalcium phosphate; Ca-P) phosphorus sources (109.80 ± 8.9 mg/L vs. 57.5 ± 7.9 mg/L, p < 0.05). Compared with the Ca-P treatment, T-41 demonstrated a stronger alkaline phosphatase (ALP) production capacity under Phytin-P treatment (34.5 ± 1.2 μmol/L/h vs. 19.8 ± 0.8 μmol/L/h, p < 0.05). Meanwhile, the production of oxalic acid, maleic acid, and succinic acid was higher under Phytin-P treatment (p < 0.05). Transcriptomic and metabolomic analysis revealed that different phosphorus sources altered metabolic pathways such as galactose metabolism, glyoxylate and dicarboxylic acid metabolism, and ascorbate and aldolate metabolism. Key metabolites like myo-inositol, 2-oxoglutarate, and pyruvate were found to impact the performance of T. gibbosa T-41 differently under the two P sources. Notably, synthesis in Ca-P vs. Pytin-P, T-41 upregulated genes involved in myo-inositol synthesis, potentially enhancing its P-solubilizing ability. These results provide new insights into the molecular mechanisms of PSF at the transcriptomic and metabolomic levels, laying a theoretical foundation for the broader application of PSF as bio-phosphorus fertilizers in the future.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1520459/fullphosphate solubilizing fungitranscriptomic analysismetabolomic analaysisphosphorus solubilization mechanismbio-phosphate fertilizer |
| spellingShingle | Yulan Chen Akasha Farooq XieLuyao Wei Leitao Qin Yong Wang Lingzi Zhang Quanju Xiang Ke Zhao Xiumei Yu Qiang Chen Xue Gao Tashi Nyima Petri Penttinen Yunfu Gu Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosa Frontiers in Microbiology phosphate solubilizing fungi transcriptomic analysis metabolomic analaysis phosphorus solubilization mechanism bio-phosphate fertilizer |
| title | Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosa |
| title_full | Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosa |
| title_fullStr | Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosa |
| title_full_unstemmed | Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosa |
| title_short | Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosa |
| title_sort | transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in trametes gibbosa |
| topic | phosphate solubilizing fungi transcriptomic analysis metabolomic analaysis phosphorus solubilization mechanism bio-phosphate fertilizer |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1520459/full |
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