The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study

Lake Beysehir, located in the Central Anatolia Region, is the biggest lake in Central Anatolia, providing drinking water and irrigation. It is Turkey's third-largest lake and the largest freshwater lake. Its waters are fresh water and its depth is around 8-9 m at most. The deepest point is ment...

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Main Authors: Ercan Arıcan, Hatice Kübra Kızıl Pat, Neşe Akçay, Sultan Fidan Pedük, Fahri Pat
Format: Article
Language:English
Published: Çanakkale Onsekiz Mart University 2023-09-01
Series:Journal of Advanced Research in Natural and Applied Sciences
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Online Access:https://dergipark.org.tr/en/download/article-file/2826884
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author Ercan Arıcan
Hatice Kübra Kızıl Pat
Neşe Akçay
Sultan Fidan Pedük
Fahri Pat
author_facet Ercan Arıcan
Hatice Kübra Kızıl Pat
Neşe Akçay
Sultan Fidan Pedük
Fahri Pat
author_sort Ercan Arıcan
collection DOAJ
description Lake Beysehir, located in the Central Anatolia Region, is the biggest lake in Central Anatolia, providing drinking water and irrigation. It is Turkey's third-largest lake and the largest freshwater lake. Its waters are fresh water and its depth is around 8-9 m at most. The deepest point is mentioned as 12, 14, and 15 m in different sources. Although there is a lake used by such a large population in our country, there are not enough studies on microbial diversity, so revealing the diversity is important for the lake and the population around the lake. Next-generation sequencing (NGS) research has made it possible to sequence RNA and DNA considerably quicker and cheaply than in by assessing the PCR amplicon, we utilized NGS high-throughput methods for the metagenomic research of Lake Beyşehir sequences of 16S rDNA (regions V3–V4). The analyzes revealed the phylum Proteobacteria, Verrucomicrobia, Bacteroidetes and Actinobacteria.
format Article
id doaj-art-143d55bcaffa4a49b396dc08d12e980f
institution Kabale University
issn 2757-5195
language English
publishDate 2023-09-01
publisher Çanakkale Onsekiz Mart University
record_format Article
series Journal of Advanced Research in Natural and Applied Sciences
spelling doaj-art-143d55bcaffa4a49b396dc08d12e980f2025-02-05T17:57:34ZengÇanakkale Onsekiz Mart UniversityJournal of Advanced Research in Natural and Applied Sciences2757-51952023-09-019371972910.28979/jarnas.1217912453The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics StudyErcan Arıcan0https://orcid.org/0000-0002-1676-5919Hatice Kübra Kızıl Pathttps://orcid.org/0000-0002-2663-1199Neşe Akçay1https://orcid.org/0000-0002-1510-7244Sultan Fidan Pedük2https://orcid.org/0000-0001-6975-2700Fahri Pat3https://orcid.org/0000-0001-9858-0012İstanbul Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik BölümüİSTANBUL ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, MOLEKÜLER BİYOTEKNOLOJİ VE GENETİK (DR)İSTANBUL ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, MOLEKÜLER BİYOLOJİ VE GENETİK (DR)İSTANBUL ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, MOLEKÜLER BİYOLOJİ VE GENETİK (DR)Lake Beysehir, located in the Central Anatolia Region, is the biggest lake in Central Anatolia, providing drinking water and irrigation. It is Turkey's third-largest lake and the largest freshwater lake. Its waters are fresh water and its depth is around 8-9 m at most. The deepest point is mentioned as 12, 14, and 15 m in different sources. Although there is a lake used by such a large population in our country, there are not enough studies on microbial diversity, so revealing the diversity is important for the lake and the population around the lake. Next-generation sequencing (NGS) research has made it possible to sequence RNA and DNA considerably quicker and cheaply than in by assessing the PCR amplicon, we utilized NGS high-throughput methods for the metagenomic research of Lake Beyşehir sequences of 16S rDNA (regions V3–V4). The analyzes revealed the phylum Proteobacteria, Verrucomicrobia, Bacteroidetes and Actinobacteria.https://dergipark.org.tr/en/download/article-file/2826884metagenomicngs16s rrnaecologymicrobial diversitymetagenomicngs16s rrnaecologymicrobial diversity
spellingShingle Ercan Arıcan
Hatice Kübra Kızıl Pat
Neşe Akçay
Sultan Fidan Pedük
Fahri Pat
The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study
Journal of Advanced Research in Natural and Applied Sciences
metagenomic
ngs
16s rrna
ecology
microbial diversity
metagenomic
ngs
16s rrna
ecology
microbial diversity
title The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study
title_full The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study
title_fullStr The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study
title_full_unstemmed The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study
title_short The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study
title_sort characterization of prokaryotic diversity in lake beysehir using a 16s metagenomics study
topic metagenomic
ngs
16s rrna
ecology
microbial diversity
metagenomic
ngs
16s rrna
ecology
microbial diversity
url https://dergipark.org.tr/en/download/article-file/2826884
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