The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary Tumors
Purpose. Nonfunctioning gonadotropic pituitary neuroendocrine tumors (PitNETs) are among the most frequent neoplasms of pituitary gland. Although PitNETs are commonly considered benign, a notable part of patients suffer from tumor recurrence after treatment. Invasive growth of pituitary tumor is amo...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2020/3730657 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560089061392384 |
|---|---|
| author | Joanna Boresowicz Paulina Kober Natalia Rusetska Maria Maksymowicz Agnieszka Paziewska Michalina Dąbrowska Natalia Zeber-Lubecka Jacek Kunicki Wiesław Bonicki Jerzy Ostrowski Janusz A. Siedlecki Mateusz Bujko |
| author_facet | Joanna Boresowicz Paulina Kober Natalia Rusetska Maria Maksymowicz Agnieszka Paziewska Michalina Dąbrowska Natalia Zeber-Lubecka Jacek Kunicki Wiesław Bonicki Jerzy Ostrowski Janusz A. Siedlecki Mateusz Bujko |
| author_sort | Joanna Boresowicz |
| collection | DOAJ |
| description | Purpose. Nonfunctioning gonadotropic pituitary neuroendocrine tumors (PitNETs) are among the most frequent neoplasms of pituitary gland. Although PitNETs are commonly considered benign, a notable part of patients suffer from tumor recurrence after treatment. Invasive growth of pituitary tumor is among the most important prognostic factors. Since molecular features of invasiveness are of potential clinical usefulness, this study was aimed to verify whether invasive and noninvasive nonfunctioning gonadotropic PitNETs differ in the miRNA expression profile and whether the differences could provide a possible molecular classifier. Methods. miRNA profiles were determined in 20 patients (11 invasive and 9 noninvasive tumors) using next-generation sequencing. The expression of selected miRNAs was assessed in the independent cohort of 80 patients with qRT-PCR. Results. When miRNA profiles of invasive and noninvasive tumors were compared, 29 miRNAs were found differentially expressed. Hsa-miR-184, hsa-miR-181a-2-3p, hsa-miR-93-3p, hsa-miR-574-5p, hsa-miR-185-5p, and hsa-miR-3200-5p showed a potential clinical value according to ROC curve analysis. Unfortunately, differential expression of only hsa-miR-185-5p was confirmed in the validation cohort, with AUG at 0.654. Conclusion. Differences in miRNAs expression profiles in invasive and noninvasive gonadotropic PitNETs are slight and the level of miRNA expression seems not to be applicable as useful classifier of tumor invasiveness. |
| format | Article |
| id | doaj-art-142ab50e77c344a697d77d4d168791d8 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-142ab50e77c344a697d77d4d168791d82025-02-03T01:28:25ZengWileyInternational Journal of Endocrinology1687-83371687-83452020-01-01202010.1155/2020/37306573730657The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary TumorsJoanna Boresowicz0Paulina Kober1Natalia Rusetska2Maria Maksymowicz3Agnieszka Paziewska4Michalina Dąbrowska5Natalia Zeber-Lubecka6Jacek Kunicki7Wiesław Bonicki8Jerzy Ostrowski9Janusz A. Siedlecki10Mateusz Bujko11Department of Molecular and Translational Oncology, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Molecular and Translational Oncology, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Molecular and Translational Oncology, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Gastroenterology, Hepatology and Clinical Oncology, Medical Center for Postgraduate Education, Warsaw, PolandDepartment of Neurosurgery, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Neurosurgery, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Molecular and Translational Oncology, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Molecular and Translational Oncology, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandPurpose. Nonfunctioning gonadotropic pituitary neuroendocrine tumors (PitNETs) are among the most frequent neoplasms of pituitary gland. Although PitNETs are commonly considered benign, a notable part of patients suffer from tumor recurrence after treatment. Invasive growth of pituitary tumor is among the most important prognostic factors. Since molecular features of invasiveness are of potential clinical usefulness, this study was aimed to verify whether invasive and noninvasive nonfunctioning gonadotropic PitNETs differ in the miRNA expression profile and whether the differences could provide a possible molecular classifier. Methods. miRNA profiles were determined in 20 patients (11 invasive and 9 noninvasive tumors) using next-generation sequencing. The expression of selected miRNAs was assessed in the independent cohort of 80 patients with qRT-PCR. Results. When miRNA profiles of invasive and noninvasive tumors were compared, 29 miRNAs were found differentially expressed. Hsa-miR-184, hsa-miR-181a-2-3p, hsa-miR-93-3p, hsa-miR-574-5p, hsa-miR-185-5p, and hsa-miR-3200-5p showed a potential clinical value according to ROC curve analysis. Unfortunately, differential expression of only hsa-miR-185-5p was confirmed in the validation cohort, with AUG at 0.654. Conclusion. Differences in miRNAs expression profiles in invasive and noninvasive gonadotropic PitNETs are slight and the level of miRNA expression seems not to be applicable as useful classifier of tumor invasiveness.http://dx.doi.org/10.1155/2020/3730657 |
| spellingShingle | Joanna Boresowicz Paulina Kober Natalia Rusetska Maria Maksymowicz Agnieszka Paziewska Michalina Dąbrowska Natalia Zeber-Lubecka Jacek Kunicki Wiesław Bonicki Jerzy Ostrowski Janusz A. Siedlecki Mateusz Bujko The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary Tumors International Journal of Endocrinology |
| title | The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary Tumors |
| title_full | The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary Tumors |
| title_fullStr | The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary Tumors |
| title_full_unstemmed | The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary Tumors |
| title_short | The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary Tumors |
| title_sort | search of mirna related to invasive growth of nonfunctioning gonadotropic pituitary tumors |
| url | http://dx.doi.org/10.1155/2020/3730657 |
| work_keys_str_mv | AT joannaboresowicz thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT paulinakober thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT nataliarusetska thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT mariamaksymowicz thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT agnieszkapaziewska thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT michalinadabrowska thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT nataliazeberlubecka thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT jacekkunicki thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT wiesławbonicki thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT jerzyostrowski thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT januszasiedlecki thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT mateuszbujko thesearchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT joannaboresowicz searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT paulinakober searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT nataliarusetska searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT mariamaksymowicz searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT agnieszkapaziewska searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT michalinadabrowska searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT nataliazeberlubecka searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT jacekkunicki searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT wiesławbonicki searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT jerzyostrowski searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT januszasiedlecki searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors AT mateuszbujko searchofmirnarelatedtoinvasivegrowthofnonfunctioninggonadotropicpituitarytumors |