The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA Nanoparticles
<b>Background/Objective</b>: Cationic polymers were shown to assemble with negatively charged proteins yielding nanoparticles (NPs). Poly-diallyl-dimethyl-ammonium chloride (PDDA) combined with ovalbumin (OVA) yielded a stable colloidal dispersion (OVA/PDDA-NPs) eliciting significant ant...
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2025-01-01
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| author | Daniele R. Pereira Yunys Pérez-Betancourt Bianca C. L. F. Távora Geraldo S. Magalhães Ana Maria Carmona-Ribeiro Eliana L. Faquim-Mauro |
| author_facet | Daniele R. Pereira Yunys Pérez-Betancourt Bianca C. L. F. Távora Geraldo S. Magalhães Ana Maria Carmona-Ribeiro Eliana L. Faquim-Mauro |
| author_sort | Daniele R. Pereira |
| collection | DOAJ |
| description | <b>Background/Objective</b>: Cationic polymers were shown to assemble with negatively charged proteins yielding nanoparticles (NPs). Poly-diallyl-dimethyl-ammonium chloride (PDDA) combined with ovalbumin (OVA) yielded a stable colloidal dispersion (OVA/PDDA-NPs) eliciting significant anti-OVA immune response. Dendritic cells (DCs), as sentinels of foreign antigens, exert a crucial role in the antigen-specific immune response. Here, we aimed to evaluate the involvement of DCs in the immune response induced by OVA/PDDA. <b>Methods</b>: In vivo experiments were used to assess the ability of OVA/PDDA-NPs to induce anti-OVA antibodies by ELISA, as well as plasma cells and memory B cells using flow cytometry. Additionally, DC migration to draining lymph nodes following OVA/PDDA-NP immunization was evaluated by flow cytometry. In vitro experiments using bone marrow-derived DCs (BM-DCs) were used to analyze the binding and uptake of OVA/PDDA-NPs, DC maturation status, and their antigen-presenting capacity. <b>Results:</b> Our data confirmed the potent effect of OVA/PDDA-NPs inducing anti-OVA IgG1 and IgG2a antibodies with increased CD19<sup>+</sup>CD138<sup>+</sup> plasma cells and CD19<sup>+</sup>CD38<sup>+</sup>CD27<sup>+</sup> memory cells in immunized mice. OVA/PDDA-NPs induced DC maturation and migration to draining lymph nodes. The in vitro results showed higher binding and the uptake of OVA/PDDA-NPs by BM-DCs. In addition, the NPs were able to induce the upregulation of costimulatory and MHC-II molecules on DCs, as well as TNF-α and IL-12 production. Higher OVA-specific T cell proliferation was promoted by BM-DCs incubated with OVA/PDDA-NPs. <b>Conclusions</b>: The data showed the central role of DCs in the induction of antigen-specific immune response by OVA-PDDA-NPs, thus proving that these NPs are a potent adjuvant for subunit vaccine design. |
| format | Article |
| id | doaj-art-142440faa41a4d98ae64850aa109b851 |
| institution | Kabale University |
| issn | 2076-393X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-142440faa41a4d98ae64850aa109b8512025-01-24T13:51:52ZengMDPI AGVaccines2076-393X2025-01-011317610.3390/vaccines13010076The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA NanoparticlesDaniele R. Pereira0Yunys Pérez-Betancourt1Bianca C. L. F. Távora2Geraldo S. Magalhães3Ana Maria Carmona-Ribeiro4Eliana L. Faquim-Mauro5Laboratory of Immunopathology, Butantan Institute, São Paulo 05585-000, BrazilDepartment of Biochemistry, Chemistry Institute, University of São Paulo, São Paulo 04021-001, BrazilLaboratory of Immunopathology, Butantan Institute, São Paulo 05585-000, BrazilLaboratory of Immunopathology, Butantan Institute, São Paulo 05585-000, BrazilDepartment of Biochemistry, Chemistry Institute, University of São Paulo, São Paulo 04021-001, BrazilLaboratory of Immunopathology, Butantan Institute, São Paulo 05585-000, Brazil<b>Background/Objective</b>: Cationic polymers were shown to assemble with negatively charged proteins yielding nanoparticles (NPs). Poly-diallyl-dimethyl-ammonium chloride (PDDA) combined with ovalbumin (OVA) yielded a stable colloidal dispersion (OVA/PDDA-NPs) eliciting significant anti-OVA immune response. Dendritic cells (DCs), as sentinels of foreign antigens, exert a crucial role in the antigen-specific immune response. Here, we aimed to evaluate the involvement of DCs in the immune response induced by OVA/PDDA. <b>Methods</b>: In vivo experiments were used to assess the ability of OVA/PDDA-NPs to induce anti-OVA antibodies by ELISA, as well as plasma cells and memory B cells using flow cytometry. Additionally, DC migration to draining lymph nodes following OVA/PDDA-NP immunization was evaluated by flow cytometry. In vitro experiments using bone marrow-derived DCs (BM-DCs) were used to analyze the binding and uptake of OVA/PDDA-NPs, DC maturation status, and their antigen-presenting capacity. <b>Results:</b> Our data confirmed the potent effect of OVA/PDDA-NPs inducing anti-OVA IgG1 and IgG2a antibodies with increased CD19<sup>+</sup>CD138<sup>+</sup> plasma cells and CD19<sup>+</sup>CD38<sup>+</sup>CD27<sup>+</sup> memory cells in immunized mice. OVA/PDDA-NPs induced DC maturation and migration to draining lymph nodes. The in vitro results showed higher binding and the uptake of OVA/PDDA-NPs by BM-DCs. In addition, the NPs were able to induce the upregulation of costimulatory and MHC-II molecules on DCs, as well as TNF-α and IL-12 production. Higher OVA-specific T cell proliferation was promoted by BM-DCs incubated with OVA/PDDA-NPs. <b>Conclusions</b>: The data showed the central role of DCs in the induction of antigen-specific immune response by OVA-PDDA-NPs, thus proving that these NPs are a potent adjuvant for subunit vaccine design.https://www.mdpi.com/2076-393X/13/1/76nanoparticlespoly-diallyl-dimethyl-ammonium chloride (PDDA)dendritic celladjuvantimmune responsevaccine |
| spellingShingle | Daniele R. Pereira Yunys Pérez-Betancourt Bianca C. L. F. Távora Geraldo S. Magalhães Ana Maria Carmona-Ribeiro Eliana L. Faquim-Mauro The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA Nanoparticles Vaccines nanoparticles poly-diallyl-dimethyl-ammonium chloride (PDDA) dendritic cell adjuvant immune response vaccine |
| title | The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA Nanoparticles |
| title_full | The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA Nanoparticles |
| title_fullStr | The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA Nanoparticles |
| title_full_unstemmed | The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA Nanoparticles |
| title_short | The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA Nanoparticles |
| title_sort | role of dendritic cells in adaptive immune response induced by ova pdda nanoparticles |
| topic | nanoparticles poly-diallyl-dimethyl-ammonium chloride (PDDA) dendritic cell adjuvant immune response vaccine |
| url | https://www.mdpi.com/2076-393X/13/1/76 |
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