Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation
Danhong Huayu Koufuye (DHK), a traditional Chinese prescription, is used to treat central retinal vein occlusion clinically. We previously reported that DHK prevented diabetic retinopathy (DR) in rats. Moreover, we found that it protected endothelial cells from hyperglycemia-induced apoptosis throug...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/3059763 |
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| author | Wenpei Chen Xiaolan Yao Chenghao Zhou Ziyang Zhang Gang Gui Baoqin Lin |
| author_facet | Wenpei Chen Xiaolan Yao Chenghao Zhou Ziyang Zhang Gang Gui Baoqin Lin |
| author_sort | Wenpei Chen |
| collection | DOAJ |
| description | Danhong Huayu Koufuye (DHK), a traditional Chinese prescription, is used to treat central retinal vein occlusion clinically. We previously reported that DHK prevented diabetic retinopathy (DR) in rats. Moreover, we found that it protected endothelial cells from hyperglycemia-induced apoptosis through antioxidation and anti-inflammation. Here, we investigated whether antioxidative and anti-inflammatory activities of DHK contributed to its therapeutic effect on DR in streptozotocin- (STZ-) induced diabetic rats. DHK significantly blocked the breakdown of the blood-retinal barrier (BRB) and increased the thickness of the inner nuclear layer (INL), as well as suppressed the swelling of the ganglion cell layer (GCL) in diabetic retinas. DHK remarkably increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in plasma, and decreased serum level of nitric oxide (NO). Moreover, DHK markedly reduced the serum levels of vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1). Furthermore, DHK significantly downregulated protein expressions of VEGF and inducible NO synthase (iNOS) and mRNA expression of ICAM-1 in retinas. These results suggest that the antioxidative and anti-inflammatory activities of DHK may be important mechanisms involved in the protective effect of DHK on DR in STZ-induced diabetic rats. |
| format | Article |
| id | doaj-art-140f27b9c6b645e592abe83c60e8c2be |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-140f27b9c6b645e592abe83c60e8c2be2025-02-03T01:30:38ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/30597633059763Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-InflammationWenpei Chen0Xiaolan Yao1Chenghao Zhou2Ziyang Zhang3Gang Gui4Baoqin Lin5School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDanhong Huayu Koufuye (DHK), a traditional Chinese prescription, is used to treat central retinal vein occlusion clinically. We previously reported that DHK prevented diabetic retinopathy (DR) in rats. Moreover, we found that it protected endothelial cells from hyperglycemia-induced apoptosis through antioxidation and anti-inflammation. Here, we investigated whether antioxidative and anti-inflammatory activities of DHK contributed to its therapeutic effect on DR in streptozotocin- (STZ-) induced diabetic rats. DHK significantly blocked the breakdown of the blood-retinal barrier (BRB) and increased the thickness of the inner nuclear layer (INL), as well as suppressed the swelling of the ganglion cell layer (GCL) in diabetic retinas. DHK remarkably increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in plasma, and decreased serum level of nitric oxide (NO). Moreover, DHK markedly reduced the serum levels of vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1). Furthermore, DHK significantly downregulated protein expressions of VEGF and inducible NO synthase (iNOS) and mRNA expression of ICAM-1 in retinas. These results suggest that the antioxidative and anti-inflammatory activities of DHK may be important mechanisms involved in the protective effect of DHK on DR in STZ-induced diabetic rats.http://dx.doi.org/10.1155/2017/3059763 |
| spellingShingle | Wenpei Chen Xiaolan Yao Chenghao Zhou Ziyang Zhang Gang Gui Baoqin Lin Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation Mediators of Inflammation |
| title | Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation |
| title_full | Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation |
| title_fullStr | Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation |
| title_full_unstemmed | Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation |
| title_short | Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation |
| title_sort | danhong huayu koufuye prevents diabetic retinopathy in streptozotocin induced diabetic rats via antioxidation and anti inflammation |
| url | http://dx.doi.org/10.1155/2017/3059763 |
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