Postharvest senescence profiling in broccoli using Tandem Mass Tag-based proteomics
Harvesting fresh produce induces countless changes in different metabolic processes, initiating senescence of the plant tissue. Understanding the changes in protein expression and activity during postharvest senescence can help identify the networks that contribute to quality loss and shelf-life red...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2024-01-01
|
| Series: | Vegetable Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/vegres-0024-0009 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850148462490812416 |
|---|---|
| author | Yogesh K. Ahlawat Utsab Ghimire Song Li Prakash Timilsena Eleni D. Pliakoni Jeffrey K. Brecht Tie Liu |
| author_facet | Yogesh K. Ahlawat Utsab Ghimire Song Li Prakash Timilsena Eleni D. Pliakoni Jeffrey K. Brecht Tie Liu |
| author_sort | Yogesh K. Ahlawat |
| collection | DOAJ |
| description | Harvesting fresh produce induces countless changes in different metabolic processes, initiating senescence of the plant tissue. Understanding the changes in protein expression and activity during postharvest senescence can help identify the networks that contribute to quality loss and shelf-life reduction in produce. Identifying these proteins can lead to strategies for extending fruits' and vegetables' freshness and shelf life. In this study, we investigated the proteome-level changes in harvested broccoli florets stored at either room temperature (25 °C) or in refrigerated conditions (4 °C) using Tandem Mass Tag technology (TMT). A lower storage temperature reduced the levels of proteins involved in ubiquitin-dependent and ubiquitin-independent protein degradation pathways mediated by the 20S and 26S proteasomes and in the RNA-Induced Silencing Complex (RISC) associated with the small interfering RNA (siRNA) pathway. Moreover, we identified protein kinases, transcription factors, and dehydration-related proteins, as well as mapped chemical interaction networks and key pathways active during broccoli senescence. Pathways related to autophagy, ribosome biosynthesis, and carbon metabolism were significantly downregulated under cold temperatures. Proteins related to chlorophyll and reactive oxygen species (ROS) pathways were differentially expressed in harvested broccoli stored at room temperature. The current report facilitates a comprehensive understanding of the complex regulatory orchestration that builds a protein network that could be used for future endeavors in improving the postharvest shelf life of broccoli. |
| format | Article |
| id | doaj-art-13f86871f55a429aa3d86bf33e6ab7f1 |
| institution | OA Journals |
| issn | 2769-0520 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Vegetable Research |
| spelling | doaj-art-13f86871f55a429aa3d86bf33e6ab7f12025-08-20T02:27:15ZengMaximum Academic PressVegetable Research2769-05202024-01-014111210.48130/vegres-0024-0009vegres-0024-0009Postharvest senescence profiling in broccoli using Tandem Mass Tag-based proteomicsYogesh K. Ahlawat0Utsab Ghimire1Song Li2Prakash Timilsena3Eleni D. Pliakoni4Jeffrey K. Brecht5Tie Liu6Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USAHorticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USASchool of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA 24060, USASchool of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA 24060, USAKansas State University, Olathe, KS 66051, USAHorticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USAHorticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USAHarvesting fresh produce induces countless changes in different metabolic processes, initiating senescence of the plant tissue. Understanding the changes in protein expression and activity during postharvest senescence can help identify the networks that contribute to quality loss and shelf-life reduction in produce. Identifying these proteins can lead to strategies for extending fruits' and vegetables' freshness and shelf life. In this study, we investigated the proteome-level changes in harvested broccoli florets stored at either room temperature (25 °C) or in refrigerated conditions (4 °C) using Tandem Mass Tag technology (TMT). A lower storage temperature reduced the levels of proteins involved in ubiquitin-dependent and ubiquitin-independent protein degradation pathways mediated by the 20S and 26S proteasomes and in the RNA-Induced Silencing Complex (RISC) associated with the small interfering RNA (siRNA) pathway. Moreover, we identified protein kinases, transcription factors, and dehydration-related proteins, as well as mapped chemical interaction networks and key pathways active during broccoli senescence. Pathways related to autophagy, ribosome biosynthesis, and carbon metabolism were significantly downregulated under cold temperatures. Proteins related to chlorophyll and reactive oxygen species (ROS) pathways were differentially expressed in harvested broccoli stored at room temperature. The current report facilitates a comprehensive understanding of the complex regulatory orchestration that builds a protein network that could be used for future endeavors in improving the postharvest shelf life of broccoli.https://www.maxapress.com/article/doi/10.48130/vegres-0024-0009postharvestsenescencesbroccolisproteomics |
| spellingShingle | Yogesh K. Ahlawat Utsab Ghimire Song Li Prakash Timilsena Eleni D. Pliakoni Jeffrey K. Brecht Tie Liu Postharvest senescence profiling in broccoli using Tandem Mass Tag-based proteomics Vegetable Research postharvest senescences broccolis proteomics |
| title | Postharvest senescence profiling in broccoli using Tandem Mass Tag-based proteomics |
| title_full | Postharvest senescence profiling in broccoli using Tandem Mass Tag-based proteomics |
| title_fullStr | Postharvest senescence profiling in broccoli using Tandem Mass Tag-based proteomics |
| title_full_unstemmed | Postharvest senescence profiling in broccoli using Tandem Mass Tag-based proteomics |
| title_short | Postharvest senescence profiling in broccoli using Tandem Mass Tag-based proteomics |
| title_sort | postharvest senescence profiling in broccoli using tandem mass tag based proteomics |
| topic | postharvest senescences broccolis proteomics |
| url | https://www.maxapress.com/article/doi/10.48130/vegres-0024-0009 |
| work_keys_str_mv | AT yogeshkahlawat postharvestsenescenceprofilinginbroccoliusingtandemmasstagbasedproteomics AT utsabghimire postharvestsenescenceprofilinginbroccoliusingtandemmasstagbasedproteomics AT songli postharvestsenescenceprofilinginbroccoliusingtandemmasstagbasedproteomics AT prakashtimilsena postharvestsenescenceprofilinginbroccoliusingtandemmasstagbasedproteomics AT elenidpliakoni postharvestsenescenceprofilinginbroccoliusingtandemmasstagbasedproteomics AT jeffreykbrecht postharvestsenescenceprofilinginbroccoliusingtandemmasstagbasedproteomics AT tieliu postharvestsenescenceprofilinginbroccoliusingtandemmasstagbasedproteomics |