Insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria-dependent pathways of apoptosis
Pesticides are always used in the environment, the unexpected effects of pesticides on the environment and non-target organisms need to be continuously studied. Insecticide chlorfenapyr (Chl) is widely used in agriculture and also recommended for public health use (e.g., providing protection from ma...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015781 |
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| author | Longfei Wang Zheng Qu Yifan Xu Guangqing Yu Xiangyang Liu Meizi Wang Shiheng An Xinming Yin Risong Na Youwu Hao |
| author_facet | Longfei Wang Zheng Qu Yifan Xu Guangqing Yu Xiangyang Liu Meizi Wang Shiheng An Xinming Yin Risong Na Youwu Hao |
| author_sort | Longfei Wang |
| collection | DOAJ |
| description | Pesticides are always used in the environment, the unexpected effects of pesticides on the environment and non-target organisms need to be continuously studied. Insecticide chlorfenapyr (Chl) is widely used in agriculture and also recommended for public health use (e.g., providing protection from malaria). Here we study toxic effects of Chl on human alveolar carcinoma cells (A549) and human normal liver cells (L02) in vitro. Chl's ability to induce DNA damage and apoptosis in human cells was confirmed through alkaline comet assay, immunofluorescence assay, and flow cytometric analysis. Further research showed that Chl induced mitochondrial damage (the collapse of mitochondrial membrane potential and the opening of mitochondrial permeability transition pore) with up-regulated expression of Bax/Bcl-2 leads to the release of cytochrome c from mitochondria which in turn activated the apoptotic pathway. Meanwhile, the key protein PARP is cleaved during apoptosis, resulting in the inhibition of DNA damage repair. In short, human A549 and L02 cells exposed to Chl were experiencing DNA damage and apoptosis linked to mitochondria. The results of this study supply theoretical understanding of Chl's toxicity on human cells, and can attract attention on the potential threat of insecticide Chl to human health. |
| format | Article |
| id | doaj-art-13ce686c0ee34cf9b3b0c80d825bda6c |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-13ce686c0ee34cf9b3b0c80d825bda6c2025-01-23T05:25:48ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117502Insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria-dependent pathways of apoptosisLongfei Wang0Zheng Qu1Yifan Xu2Guangqing Yu3Xiangyang Liu4Meizi Wang5Shiheng An6Xinming Yin7Risong Na8Youwu Hao9National Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaNational Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China; Corresponding author.Pesticides are always used in the environment, the unexpected effects of pesticides on the environment and non-target organisms need to be continuously studied. Insecticide chlorfenapyr (Chl) is widely used in agriculture and also recommended for public health use (e.g., providing protection from malaria). Here we study toxic effects of Chl on human alveolar carcinoma cells (A549) and human normal liver cells (L02) in vitro. Chl's ability to induce DNA damage and apoptosis in human cells was confirmed through alkaline comet assay, immunofluorescence assay, and flow cytometric analysis. Further research showed that Chl induced mitochondrial damage (the collapse of mitochondrial membrane potential and the opening of mitochondrial permeability transition pore) with up-regulated expression of Bax/Bcl-2 leads to the release of cytochrome c from mitochondria which in turn activated the apoptotic pathway. Meanwhile, the key protein PARP is cleaved during apoptosis, resulting in the inhibition of DNA damage repair. In short, human A549 and L02 cells exposed to Chl were experiencing DNA damage and apoptosis linked to mitochondria. The results of this study supply theoretical understanding of Chl's toxicity on human cells, and can attract attention on the potential threat of insecticide Chl to human health.http://www.sciencedirect.com/science/article/pii/S0147651324015781ChlorfenapyrCytotoxicityDNA damageApoptosisSafety risk |
| spellingShingle | Longfei Wang Zheng Qu Yifan Xu Guangqing Yu Xiangyang Liu Meizi Wang Shiheng An Xinming Yin Risong Na Youwu Hao Insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria-dependent pathways of apoptosis Ecotoxicology and Environmental Safety Chlorfenapyr Cytotoxicity DNA damage Apoptosis Safety risk |
| title | Insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria-dependent pathways of apoptosis |
| title_full | Insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria-dependent pathways of apoptosis |
| title_fullStr | Insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria-dependent pathways of apoptosis |
| title_full_unstemmed | Insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria-dependent pathways of apoptosis |
| title_short | Insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria-dependent pathways of apoptosis |
| title_sort | insecticide chlorfenapyr confers induced toxicity in human cells through mitochondria dependent pathways of apoptosis |
| topic | Chlorfenapyr Cytotoxicity DNA damage Apoptosis Safety risk |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015781 |
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