Antibiofilm of metabolites of the Proteus myxofaciens JB 20B for the prevention and treatment of vaname shrimp infected with Vibrio harveyi bacteria
Vibrio harveyi is one of the common bacteria that cause disease in vaname shrimp. The purpose of this study is to determine the efficacy of bacterial metabolites Proteus myxofaciens JB 20B as an antibiofilm agent in the prevention and treatment of V. harveyi-infected vaname shrimp (Litopenaeus vann...
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Asosiasi Sains Akuakultur Indonesia
2025-01-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/57225 |
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| author | Nanda Tri Rezeki Dinamella Wahjuningrum Widanarni Widanarni Diana Elizabeth Waturangi |
| author_facet | Nanda Tri Rezeki Dinamella Wahjuningrum Widanarni Widanarni Diana Elizabeth Waturangi |
| author_sort | Nanda Tri Rezeki |
| collection | DOAJ |
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Vibrio harveyi is one of the common bacteria that cause disease in vaname shrimp. The purpose of this study is to determine the efficacy of bacterial metabolites Proteus myxofaciens JB 20B as an antibiofilm agent in the prevention and treatment of V. harveyi-infected vaname shrimp (Litopenaeus vannamei). The research design included eight treatments and three replicates: K- (negative control), K+ (positive control), KANC and KANG (antibiotic control 50 g/100ml for preventive and treatment), PCE (preventive extract 0.1 ml/Kg feed), PCS (preventive supernatant 20 ml/Kg feed), PGE (treatment extract 0.1 ml/Kg feed), and PGS (treatment supernatant 20 ml/Kg feed). On day 15th, vaname shrimp were intramuscularly infected with 106 CFU/mL of V. harveyi. Examining clinical symptoms, viewing histology, assessing total haemocyte count, phagocytosis activity, phenoloxidase activity, respiratory burst, and growth performance. According to the findings of this study, the treatment of bacterial metabolites P. myxofaciens JB 20B (PCE, PCS, PGE, and PGS) is superior to the positive control (K+) treatment in terms of reducing shrimp hepatopancreas necrosis, increasing the value of total haemocyte count, phagocytosis activity, phenoloxidase activity, respiratory burst, and growth performance of vaname shrimp through post-infection feeding in both prevention and treatment. The use of P. myxofaciens JB 20B bacterial metabolites to control V. harveyi infection in vaname shrimp yielded the best results in the supernatant treatment.
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| format | Article |
| id | doaj-art-13b4283857fb40eda4784089f8e2a7bf |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-13b4283857fb40eda4784089f8e2a7bf2025-01-24T08:06:43ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002025-01-0124110.19027/jai.24.1.111-120Antibiofilm of metabolites of the Proteus myxofaciens JB 20B for the prevention and treatment of vaname shrimp infected with Vibrio harveyi bacteriaNanda Tri Rezeki0Dinamella Wahjuningrum1Widanarni Widanarni2Diana Elizabeth Waturangi3Aquaculture Science, Post Graduate Study Program, Faculty of Fishery and marine Sciences, IPB University, Dramaga Campus, Bogor, West Java 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, IndonesiaDepartment of Biology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia Vibrio harveyi is one of the common bacteria that cause disease in vaname shrimp. The purpose of this study is to determine the efficacy of bacterial metabolites Proteus myxofaciens JB 20B as an antibiofilm agent in the prevention and treatment of V. harveyi-infected vaname shrimp (Litopenaeus vannamei). The research design included eight treatments and three replicates: K- (negative control), K+ (positive control), KANC and KANG (antibiotic control 50 g/100ml for preventive and treatment), PCE (preventive extract 0.1 ml/Kg feed), PCS (preventive supernatant 20 ml/Kg feed), PGE (treatment extract 0.1 ml/Kg feed), and PGS (treatment supernatant 20 ml/Kg feed). On day 15th, vaname shrimp were intramuscularly infected with 106 CFU/mL of V. harveyi. Examining clinical symptoms, viewing histology, assessing total haemocyte count, phagocytosis activity, phenoloxidase activity, respiratory burst, and growth performance. According to the findings of this study, the treatment of bacterial metabolites P. myxofaciens JB 20B (PCE, PCS, PGE, and PGS) is superior to the positive control (K+) treatment in terms of reducing shrimp hepatopancreas necrosis, increasing the value of total haemocyte count, phagocytosis activity, phenoloxidase activity, respiratory burst, and growth performance of vaname shrimp through post-infection feeding in both prevention and treatment. The use of P. myxofaciens JB 20B bacterial metabolites to control V. harveyi infection in vaname shrimp yielded the best results in the supernatant treatment. https://journal.ipb.ac.id/index.php/jai/article/view/57225 |
| spellingShingle | Nanda Tri Rezeki Dinamella Wahjuningrum Widanarni Widanarni Diana Elizabeth Waturangi Antibiofilm of metabolites of the Proteus myxofaciens JB 20B for the prevention and treatment of vaname shrimp infected with Vibrio harveyi bacteria Jurnal Akuakultur Indonesia |
| title | Antibiofilm of metabolites of the Proteus myxofaciens JB 20B for the prevention and treatment of vaname shrimp infected with Vibrio harveyi bacteria |
| title_full | Antibiofilm of metabolites of the Proteus myxofaciens JB 20B for the prevention and treatment of vaname shrimp infected with Vibrio harveyi bacteria |
| title_fullStr | Antibiofilm of metabolites of the Proteus myxofaciens JB 20B for the prevention and treatment of vaname shrimp infected with Vibrio harveyi bacteria |
| title_full_unstemmed | Antibiofilm of metabolites of the Proteus myxofaciens JB 20B for the prevention and treatment of vaname shrimp infected with Vibrio harveyi bacteria |
| title_short | Antibiofilm of metabolites of the Proteus myxofaciens JB 20B for the prevention and treatment of vaname shrimp infected with Vibrio harveyi bacteria |
| title_sort | antibiofilm of metabolites of the proteus myxofaciens jb 20b for the prevention and treatment of vaname shrimp infected with vibrio harveyi bacteria |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/57225 |
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