Distinct phenotypes of salivaricin-producing Ligilactobacillus salivarius isolated from the gastrointestinal tract of broiler chickens and laying hens
Ligilactobacillus salivarius harbors bacteriocin genes in its repA-type megaplasmid, specifically salivaricin P (salP), a class IIb bacteriocin. This study aimed to differentiate 25 salP-positive Lig. salivarius strains isolated from the gastrointestinal tract (GIT) of broilers and laying hens. Resu...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011155 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591820733808640 |
|---|---|
| author | A.G. Elnar Y.J. Jang B.G. Eum M.H. Kang G.W. Hwang D.Y. Kil G.B. Kim |
| author_facet | A.G. Elnar Y.J. Jang B.G. Eum M.H. Kang G.W. Hwang D.Y. Kil G.B. Kim |
| author_sort | A.G. Elnar |
| collection | DOAJ |
| description | Ligilactobacillus salivarius harbors bacteriocin genes in its repA-type megaplasmid, specifically salivaricin P (salP), a class IIb bacteriocin. This study aimed to differentiate 25 salP-positive Lig. salivarius strains isolated from the gastrointestinal tract (GIT) of broilers and laying hens. Results showed that 12 isolates were classified as Type A, with active bacteriocins, while the rest were Type B, with no active bacteriocins. In vitro and in silico characterization of salP bacteriocins revealed narrow-spectrum antibacterial activity against Listeria monocytogenes and Enterococcus faecalis. SalP bacteriocins were predicted as positively charged, hydrophobic, small molecular weight (α, 4.097 kDa; ß, 4.285 kDa) bacteriocins with characteristic GXXXG motif. Investigation of the salP gene cluster based on genomic data revealed that Type B strains lacked the lanT and hlyD genes that encode export proteins dedicated to the modification and extracellular transport of mature salP peptides. However, two Type B strains (B4311 and B5258) showed inhibitory activity against L. monocytogenes ATCC19114. Multiplex PCR analysis and synteny mapping analysis revealed that B4311 and B5258 strains harbored the lanT gene, highlighting the importance of LanT protein in the cleavage of leader peptide and excretion of mature peptides. Further analysis revealed that the resistance of Type B strains to salP was attributable to the presence of a dedicated immunity protein, blurring the evolutionary significance of producing active bacteriocins for competitive advantage. Additionally, the loss of export proteins occurred in a polyphyletic manner, consistent with the genetic plasticity of the repA-type megaplasmid. This suggests that the loss of lanT and hlyD is likely in the presence of limited nutritional competitors. In conclusion, the observed differences in salivaricin production of Lig. salivarius exist independent of isolation host and that Type A and Type B strains can coexist in the same environment. Finally, the functional characterization of active salP allows for a better understanding of its potential to control specific bacteria in human food and animal production. |
| format | Article |
| id | doaj-art-13b3a4b34b6b48faaf8df2a7d4d0cdb7 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-13b3a4b34b6b48faaf8df2a7d4d0cdb72025-01-22T05:40:23ZengElsevierPoultry Science0032-57912025-01-011041104537Distinct phenotypes of salivaricin-producing Ligilactobacillus salivarius isolated from the gastrointestinal tract of broiler chickens and laying hensA.G. Elnar0Y.J. Jang1B.G. Eum2M.H. Kang3G.W. Hwang4D.Y. Kil5G.B. Kim6Department of Animal Science and Technology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of KoreaCorresponding author.; Department of Animal Science and Technology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of KoreaLigilactobacillus salivarius harbors bacteriocin genes in its repA-type megaplasmid, specifically salivaricin P (salP), a class IIb bacteriocin. This study aimed to differentiate 25 salP-positive Lig. salivarius strains isolated from the gastrointestinal tract (GIT) of broilers and laying hens. Results showed that 12 isolates were classified as Type A, with active bacteriocins, while the rest were Type B, with no active bacteriocins. In vitro and in silico characterization of salP bacteriocins revealed narrow-spectrum antibacterial activity against Listeria monocytogenes and Enterococcus faecalis. SalP bacteriocins were predicted as positively charged, hydrophobic, small molecular weight (α, 4.097 kDa; ß, 4.285 kDa) bacteriocins with characteristic GXXXG motif. Investigation of the salP gene cluster based on genomic data revealed that Type B strains lacked the lanT and hlyD genes that encode export proteins dedicated to the modification and extracellular transport of mature salP peptides. However, two Type B strains (B4311 and B5258) showed inhibitory activity against L. monocytogenes ATCC19114. Multiplex PCR analysis and synteny mapping analysis revealed that B4311 and B5258 strains harbored the lanT gene, highlighting the importance of LanT protein in the cleavage of leader peptide and excretion of mature peptides. Further analysis revealed that the resistance of Type B strains to salP was attributable to the presence of a dedicated immunity protein, blurring the evolutionary significance of producing active bacteriocins for competitive advantage. Additionally, the loss of export proteins occurred in a polyphyletic manner, consistent with the genetic plasticity of the repA-type megaplasmid. This suggests that the loss of lanT and hlyD is likely in the presence of limited nutritional competitors. In conclusion, the observed differences in salivaricin production of Lig. salivarius exist independent of isolation host and that Type A and Type B strains can coexist in the same environment. Finally, the functional characterization of active salP allows for a better understanding of its potential to control specific bacteria in human food and animal production.http://www.sciencedirect.com/science/article/pii/S0032579124011155BacteriocinEvolutionLigilactobacillus salivariusMegaplasmidPhylogeny |
| spellingShingle | A.G. Elnar Y.J. Jang B.G. Eum M.H. Kang G.W. Hwang D.Y. Kil G.B. Kim Distinct phenotypes of salivaricin-producing Ligilactobacillus salivarius isolated from the gastrointestinal tract of broiler chickens and laying hens Poultry Science Bacteriocin Evolution Ligilactobacillus salivarius Megaplasmid Phylogeny |
| title | Distinct phenotypes of salivaricin-producing Ligilactobacillus salivarius isolated from the gastrointestinal tract of broiler chickens and laying hens |
| title_full | Distinct phenotypes of salivaricin-producing Ligilactobacillus salivarius isolated from the gastrointestinal tract of broiler chickens and laying hens |
| title_fullStr | Distinct phenotypes of salivaricin-producing Ligilactobacillus salivarius isolated from the gastrointestinal tract of broiler chickens and laying hens |
| title_full_unstemmed | Distinct phenotypes of salivaricin-producing Ligilactobacillus salivarius isolated from the gastrointestinal tract of broiler chickens and laying hens |
| title_short | Distinct phenotypes of salivaricin-producing Ligilactobacillus salivarius isolated from the gastrointestinal tract of broiler chickens and laying hens |
| title_sort | distinct phenotypes of salivaricin producing ligilactobacillus salivarius isolated from the gastrointestinal tract of broiler chickens and laying hens |
| topic | Bacteriocin Evolution Ligilactobacillus salivarius Megaplasmid Phylogeny |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011155 |
| work_keys_str_mv | AT agelnar distinctphenotypesofsalivaricinproducingligilactobacillussalivariusisolatedfromthegastrointestinaltractofbroilerchickensandlayinghens AT yjjang distinctphenotypesofsalivaricinproducingligilactobacillussalivariusisolatedfromthegastrointestinaltractofbroilerchickensandlayinghens AT bgeum distinctphenotypesofsalivaricinproducingligilactobacillussalivariusisolatedfromthegastrointestinaltractofbroilerchickensandlayinghens AT mhkang distinctphenotypesofsalivaricinproducingligilactobacillussalivariusisolatedfromthegastrointestinaltractofbroilerchickensandlayinghens AT gwhwang distinctphenotypesofsalivaricinproducingligilactobacillussalivariusisolatedfromthegastrointestinaltractofbroilerchickensandlayinghens AT dykil distinctphenotypesofsalivaricinproducingligilactobacillussalivariusisolatedfromthegastrointestinaltractofbroilerchickensandlayinghens AT gbkim distinctphenotypesofsalivaricinproducingligilactobacillussalivariusisolatedfromthegastrointestinaltractofbroilerchickensandlayinghens |