Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review
ObjectivesThe aim of the present systematic review was to investigate the cryopreservation process of dental pulp mesenchymal stromal cells and whether cryopreservation is effective in promoting cell viability and recovery.Materials and MethodsThis systematic review was developed in accordance with...
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Korean Academy of Conservative Dentistry
2021-05-01
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| Series: | Restorative Dentistry & Endodontics |
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| Online Access: | http://rde.ac/upload/pdf/rde-46-e26.pdf |
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| author | Sabrina Moreira Paes Yasmine Mendes Pupo Bruno Cavalini Cavenago Thiago Fonseca-Silva Carolina Carvalho de Oliveira Santos |
| author_facet | Sabrina Moreira Paes Yasmine Mendes Pupo Bruno Cavalini Cavenago Thiago Fonseca-Silva Carolina Carvalho de Oliveira Santos |
| author_sort | Sabrina Moreira Paes |
| collection | DOAJ |
| description | ObjectivesThe aim of the present systematic review was to investigate the cryopreservation process of dental pulp mesenchymal stromal cells and whether cryopreservation is effective in promoting cell viability and recovery.Materials and MethodsThis systematic review was developed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and the research question was determined using the population, exposure, comparison, and outcomes strategy. Electronic searches were conducted in the PubMed, Cochrane Library, Science Direct, LILACS, and SciELO databases and in the gray literature (dissertations and thesis databases and Google Scholar) for relevant articles published up to March 2019. Clinical trial studies performed with dental pulp of human permanent or primary teeth, containing concrete information regarding the cryopreservation stages, and with cryopreservation performed for a period of at least 1 week were included in this study.ResultsThe search strategy resulted in the retrieval of 185 publications. After the application of the eligibility criteria, 21 articles were selected for a qualitative analysis.ConclusionsThe cryopreservation process must be carried out in 6 stages: tooth disinfection, pulp extraction, cell isolation, cell proliferation, cryopreservation, and thawing. In addition, it can be inferred that the use of dimethyl sulfoxide, programmable freezing, and storage in liquid nitrogen are associated with a high rate of cell viability after thawing and a high rate of cell proliferation in both primary and permanent teeth. |
| format | Article |
| id | doaj-art-13aa2c2b28f042e7861c1d8646bd8c6f |
| institution | Kabale University |
| issn | 2234-7658 2234-7666 |
| language | English |
| publishDate | 2021-05-01 |
| publisher | Korean Academy of Conservative Dentistry |
| record_format | Article |
| series | Restorative Dentistry & Endodontics |
| spelling | doaj-art-13aa2c2b28f042e7861c1d8646bd8c6f2025-08-20T03:30:40ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662021-05-01462e2610.5395/rde.2021.46.e26963Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic reviewSabrina Moreira Paes0Yasmine Mendes Pupo1Bruno Cavalini Cavenago2Thiago Fonseca-Silva3Carolina Carvalho de Oliveira Santos4Department of Restorative Dentistry, Universidade Federal do Paraná, Curitiba/PR, BrazilDepartment of Restorative Dentistry, Universidade Federal do Paraná, Curitiba/PR, BrazilDepartment of Restorative Dentistry, Universidade Federal do Paraná, Curitiba/PR, BrazilDepartment of Dentistry, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina/MG, BrazilDepartment of Restorative Dentistry, Universidade Federal do Paraná, Curitiba/PR, BrazilObjectivesThe aim of the present systematic review was to investigate the cryopreservation process of dental pulp mesenchymal stromal cells and whether cryopreservation is effective in promoting cell viability and recovery.Materials and MethodsThis systematic review was developed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and the research question was determined using the population, exposure, comparison, and outcomes strategy. Electronic searches were conducted in the PubMed, Cochrane Library, Science Direct, LILACS, and SciELO databases and in the gray literature (dissertations and thesis databases and Google Scholar) for relevant articles published up to March 2019. Clinical trial studies performed with dental pulp of human permanent or primary teeth, containing concrete information regarding the cryopreservation stages, and with cryopreservation performed for a period of at least 1 week were included in this study.ResultsThe search strategy resulted in the retrieval of 185 publications. After the application of the eligibility criteria, 21 articles were selected for a qualitative analysis.ConclusionsThe cryopreservation process must be carried out in 6 stages: tooth disinfection, pulp extraction, cell isolation, cell proliferation, cryopreservation, and thawing. In addition, it can be inferred that the use of dimethyl sulfoxide, programmable freezing, and storage in liquid nitrogen are associated with a high rate of cell viability after thawing and a high rate of cell proliferation in both primary and permanent teeth.http://rde.ac/upload/pdf/rde-46-e26.pdfcryopreservationdental pulpmesenchymal stromal cellsstem cells |
| spellingShingle | Sabrina Moreira Paes Yasmine Mendes Pupo Bruno Cavalini Cavenago Thiago Fonseca-Silva Carolina Carvalho de Oliveira Santos Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review Restorative Dentistry & Endodontics cryopreservation dental pulp mesenchymal stromal cells stem cells |
| title | Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review |
| title_full | Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review |
| title_fullStr | Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review |
| title_full_unstemmed | Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review |
| title_short | Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review |
| title_sort | cryopreservation of mesenchymal stem cells derived from dental pulp a systematic review |
| topic | cryopreservation dental pulp mesenchymal stromal cells stem cells |
| url | http://rde.ac/upload/pdf/rde-46-e26.pdf |
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