Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography
A reliable and rapid method has been developed for the determination of aflatoxin B1 (AFB1) in four kinds of feedstuffs comprising broken rice, peanuts, corn, and fishmeal. A sample preparation was carried out based on the QuEChERS method with the exclusion of the clean-up step. In this study, AFB1...
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| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/4650764 |
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| author | Sasiprapa Choochuay Jutamas Phakam Prakorn Jala Thanapoom Maneeboon Natthasit Tansakul |
| author_facet | Sasiprapa Choochuay Jutamas Phakam Prakorn Jala Thanapoom Maneeboon Natthasit Tansakul |
| author_sort | Sasiprapa Choochuay |
| collection | DOAJ |
| description | A reliable and rapid method has been developed for the determination of aflatoxin B1 (AFB1) in four kinds of feedstuffs comprising broken rice, peanuts, corn, and fishmeal. A sample preparation was carried out based on the QuEChERS method with the exclusion of the clean-up step. In this study, AFB1 was extracted using acetonitrile/methanol (40/60 v/v), followed by partitioning with sodium chloride and magnesium sulfate. High-performance liquid chromatography with precolumn derivatization and fluorescence detection was performed. The coefficients of determination were greater than 0.9800. Throughout the developed method, the recovery of all feedstuffs achieved a range of 82.50-109.85% with relative standard deviation lower than 11% for all analytes at a concentration of 20-100 ng/g. The limit of detection (LOD) ranged from 0.2 to 1.2 ng/g and limit of quantitation (LOQ) ranged from 0.3 to 1.5 ng/g. The validated method was successfully applied to a total of 120 samples. The occurrence of AFB1 contamination was found at the following concentrations: in broken rice (0.44-2.33ng/g), peanut (3.97-106.26ng/g), corn (0.88-50.29 ng/g), and fishmeal (1.06-10.35 ng/g). These results indicate that the proposed method may be useful for regularly monitoring AFB1 contamination in feedstuffs. |
| format | Article |
| id | doaj-art-1387212d8f0c435189385bc6d5e51679 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-1387212d8f0c435189385bc6d5e516792025-02-03T01:24:26ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792018-01-01201810.1155/2018/46507644650764Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid ChromatographySasiprapa Choochuay0Jutamas Phakam1Prakorn Jala2Thanapoom Maneeboon3Natthasit Tansakul4Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kasetsart University, 10900, Bangkok, ThailandDepartment of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kasetsart University, 10900, Bangkok, ThailandFaculty of Veterinary Medicine, Kamphaengsaen Campus, 73140, Nakhon Pathom, ThailandKasetsart University Research and Development Institute, 10900, Bangkok, ThailandDepartment of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kasetsart University, 10900, Bangkok, ThailandA reliable and rapid method has been developed for the determination of aflatoxin B1 (AFB1) in four kinds of feedstuffs comprising broken rice, peanuts, corn, and fishmeal. A sample preparation was carried out based on the QuEChERS method with the exclusion of the clean-up step. In this study, AFB1 was extracted using acetonitrile/methanol (40/60 v/v), followed by partitioning with sodium chloride and magnesium sulfate. High-performance liquid chromatography with precolumn derivatization and fluorescence detection was performed. The coefficients of determination were greater than 0.9800. Throughout the developed method, the recovery of all feedstuffs achieved a range of 82.50-109.85% with relative standard deviation lower than 11% for all analytes at a concentration of 20-100 ng/g. The limit of detection (LOD) ranged from 0.2 to 1.2 ng/g and limit of quantitation (LOQ) ranged from 0.3 to 1.5 ng/g. The validated method was successfully applied to a total of 120 samples. The occurrence of AFB1 contamination was found at the following concentrations: in broken rice (0.44-2.33ng/g), peanut (3.97-106.26ng/g), corn (0.88-50.29 ng/g), and fishmeal (1.06-10.35 ng/g). These results indicate that the proposed method may be useful for regularly monitoring AFB1 contamination in feedstuffs.http://dx.doi.org/10.1155/2018/4650764 |
| spellingShingle | Sasiprapa Choochuay Jutamas Phakam Prakorn Jala Thanapoom Maneeboon Natthasit Tansakul Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography International Journal of Analytical Chemistry |
| title | Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography |
| title_full | Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography |
| title_fullStr | Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography |
| title_full_unstemmed | Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography |
| title_short | Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography |
| title_sort | determination of aflatoxin b1 in feedstuffs without clean up step by high performance liquid chromatography |
| url | http://dx.doi.org/10.1155/2018/4650764 |
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