Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental Strain
The molecular basis of attenuation of foot-and-mouth disease virus (FMDV) serotype Asia1 ZB strain remains unknown. To understand the genetic changes of attenuation, we compared the entire genomes of three different rabbit-passaged attenuated ZB strains (ZB/CHA/58(att), ZBRF168, and ZBRF188) and the...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2014/978609 |
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| author | Aiguo Xin Mingwang Zhu Zhenqi Peng Qi Hu Chenhong Shi Defang Liao Jihua Wang Huachun Li |
| author_facet | Aiguo Xin Mingwang Zhu Zhenqi Peng Qi Hu Chenhong Shi Defang Liao Jihua Wang Huachun Li |
| author_sort | Aiguo Xin |
| collection | DOAJ |
| description | The molecular basis of attenuation of foot-and-mouth disease virus (FMDV) serotype Asia1 ZB strain remains unknown. To understand the genetic changes of attenuation, we compared the entire genomes of three different rabbit-passaged attenuated ZB strains (ZB/CHA/58(att), ZBRF168, and ZBRF188) and their virulent parental strains (ZBCF22 and YNBS/58). The results showed that attenuation may be brought about by 28 common amino acid substitutions in the coding region, with one nucleotide point mutation in the 5′-untranslated region (5′-UTR) and another one in the 3′-UTR. In addition, a total of 21 nucleotides silent mutations had been found after attenuation. These substitutions, alone or in combination, may be responsible for the attenuated phenotype of the ZB strain in cattle. This will contribute to elucidation of attenuating molecular basis of the FMDV ZB strain. |
| format | Article |
| id | doaj-art-1366c725f22742ccad50295a96c1c469 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-1366c725f22742ccad50295a96c1c4692025-02-03T05:50:09ZengWileyInternational Journal of Genomics2314-436X2314-43782014-01-01201410.1155/2014/978609978609Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental StrainAiguo Xin0Mingwang Zhu1Zhenqi Peng2Qi Hu3Chenhong Shi4Defang Liao5Jihua Wang6Huachun Li7Yunnan Tropical and Subtropical Animal Virus Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, ChinaYunnan Provincial Research Center for Veterinary Biological Products, Baoshan 678000, ChinaYunnan Provincial Research Center for Veterinary Biological Products, Baoshan 678000, ChinaYunnan Tropical and Subtropical Animal Virus Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, ChinaYunnan Provincial Research Center for Veterinary Biological Products, Baoshan 678000, ChinaYunnan Tropical and Subtropical Animal Virus Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, ChinaYunnan Provincial Research Center for Veterinary Biological Products, Baoshan 678000, ChinaYunnan Tropical and Subtropical Animal Virus Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, ChinaThe molecular basis of attenuation of foot-and-mouth disease virus (FMDV) serotype Asia1 ZB strain remains unknown. To understand the genetic changes of attenuation, we compared the entire genomes of three different rabbit-passaged attenuated ZB strains (ZB/CHA/58(att), ZBRF168, and ZBRF188) and their virulent parental strains (ZBCF22 and YNBS/58). The results showed that attenuation may be brought about by 28 common amino acid substitutions in the coding region, with one nucleotide point mutation in the 5′-untranslated region (5′-UTR) and another one in the 3′-UTR. In addition, a total of 21 nucleotides silent mutations had been found after attenuation. These substitutions, alone or in combination, may be responsible for the attenuated phenotype of the ZB strain in cattle. This will contribute to elucidation of attenuating molecular basis of the FMDV ZB strain.http://dx.doi.org/10.1155/2014/978609 |
| spellingShingle | Aiguo Xin Mingwang Zhu Zhenqi Peng Qi Hu Chenhong Shi Defang Liao Jihua Wang Huachun Li Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental Strain International Journal of Genomics |
| title | Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental Strain |
| title_full | Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental Strain |
| title_fullStr | Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental Strain |
| title_full_unstemmed | Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental Strain |
| title_short | Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental Strain |
| title_sort | genomic changes in an attenuated zb strain of foot and mouth disease virus serotype asia1 and comparison with its virulent parental strain |
| url | http://dx.doi.org/10.1155/2014/978609 |
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