Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells
In bone tissue engineering, various approaches have been investigated to enhance osteogenic regeneration. Previous studies have predominantly employed scaffolds with aligned structures or reduced graphene oxide (RGO) to facilitate bone regeneration. However, current scaffold designs face limitations...
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IEEE
2024-01-01
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| Series: | IEEE Open Journal of Nanotechnology |
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| Online Access: | https://ieeexplore.ieee.org/document/10769987/ |
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| author | Juo Lee Sungmin Lee Iksong Byun Myung Chul Lee Jungsil Kim Hoon Seonwoo |
| author_facet | Juo Lee Sungmin Lee Iksong Byun Myung Chul Lee Jungsil Kim Hoon Seonwoo |
| author_sort | Juo Lee |
| collection | DOAJ |
| description | In bone tissue engineering, various approaches have been investigated to enhance osteogenic regeneration. Previous studies have predominantly employed scaffolds with aligned structures or reduced graphene oxide (RGO) to facilitate bone regeneration. However, current scaffold designs face limitations in combining structural guidance with effective electromagnetic stimulation. Additionally, delivering localized stimulation within scaffolds remains a challenge in maximizing the potential of these materials for bone regeneration. To address these limitations and strengthen previous approaches, this study presents a novel strategy in tissue engineering for enhanced osteogenic differentiation. RGO-incorporated nanofibers (RGO-NFs) were fabricated via electrospinning a 10% polycaprolactone (PCL) solution with RGO concentrations varying. The random fibers were deposited on a planar surface, while the aligned fibers were deposited on a rotating drum. The morphology and orientation of the fibers were confirmed through electron microscopy. X-ray diffraction spectrometry was employed to confirm the integration of RGO and PCL. All groups demonstrated optimal cell adhesion and viability. RGO-NFs exhibited higher osteogenesis-related protein expression than PCL-only scaffolds, further enhanced by pulsed electromagnetic field (PEMF) application. The application of PEMF stimulation within aligned RGO-NFs presents a potentially more efficient alternative to existing methods, offering a novel, non-invasive therapeutic strategy for bone defect regeneration. |
| format | Article |
| id | doaj-art-13637c302bca46e3b4aa7bfede7e07f3 |
| institution | Kabale University |
| issn | 2644-1292 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Open Journal of Nanotechnology |
| spelling | doaj-art-13637c302bca46e3b4aa7bfede7e07f32025-01-25T00:03:25ZengIEEEIEEE Open Journal of Nanotechnology2644-12922024-01-01512413310.1109/OJNANO.2024.349477010769987Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem CellsJuo Lee0Sungmin Lee1https://orcid.org/0009-0002-0156-007XIksong Byun2Myung Chul Lee3https://orcid.org/0000-0002-0566-8494Jungsil Kim4https://orcid.org/0000-0001-5028-7554Hoon Seonwoo5https://orcid.org/0000-0002-1827-9359Department of Convergent Biosystems Engineering, College of Life Science and Natural Resources, Sunchon National University, Suncheon, Republic of KoreaInterdisciplinary Program in IT-Bio Convergence System, Sunchon National University, Suncheon, Republic of KoreaInterdisciplinary Program in IT-Bio Convergence System, Sunchon National University, Suncheon, Republic of KoreaDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USADepartment of Smart Bio-Industrial Mechanical Engineering, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of KoreaDepartment of Convergent Biosystems Engineering, College of Life Science and Natural Resources, Sunchon National University, Suncheon, Republic of KoreaIn bone tissue engineering, various approaches have been investigated to enhance osteogenic regeneration. Previous studies have predominantly employed scaffolds with aligned structures or reduced graphene oxide (RGO) to facilitate bone regeneration. However, current scaffold designs face limitations in combining structural guidance with effective electromagnetic stimulation. Additionally, delivering localized stimulation within scaffolds remains a challenge in maximizing the potential of these materials for bone regeneration. To address these limitations and strengthen previous approaches, this study presents a novel strategy in tissue engineering for enhanced osteogenic differentiation. RGO-incorporated nanofibers (RGO-NFs) were fabricated via electrospinning a 10% polycaprolactone (PCL) solution with RGO concentrations varying. The random fibers were deposited on a planar surface, while the aligned fibers were deposited on a rotating drum. The morphology and orientation of the fibers were confirmed through electron microscopy. X-ray diffraction spectrometry was employed to confirm the integration of RGO and PCL. All groups demonstrated optimal cell adhesion and viability. RGO-NFs exhibited higher osteogenesis-related protein expression than PCL-only scaffolds, further enhanced by pulsed electromagnetic field (PEMF) application. The application of PEMF stimulation within aligned RGO-NFs presents a potentially more efficient alternative to existing methods, offering a novel, non-invasive therapeutic strategy for bone defect regeneration.https://ieeexplore.ieee.org/document/10769987/Graphenenanofiberelectrospinningdental pulp stem cellsosteogenic differentiation |
| spellingShingle | Juo Lee Sungmin Lee Iksong Byun Myung Chul Lee Jungsil Kim Hoon Seonwoo Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells IEEE Open Journal of Nanotechnology Graphene nanofiber electrospinning dental pulp stem cells osteogenic differentiation |
| title | Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells |
| title_full | Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells |
| title_fullStr | Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells |
| title_full_unstemmed | Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells |
| title_short | Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells |
| title_sort | pulsed electromagnetic field assisting reduced graphene oxide incorporated nanofibers for osteogenic differentiation of human dental pulp stem cells |
| topic | Graphene nanofiber electrospinning dental pulp stem cells osteogenic differentiation |
| url | https://ieeexplore.ieee.org/document/10769987/ |
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