Characterization of the Active Ingredient and Prediction of the Potential Mechanism of Dahuoluo Pill via Mass Spectrometry with the Network Pharmacology Method
The Dahuoluo pill (DHLP) is a classic Chinese patent medicine used to treat rheumatoid arthritis and other conditions. However, there has been no research on the chemical components of DHLP and the mechanisms by which it ameliorates rheumatoid arthritis. Hence, we analysed the chemical components of...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/8819534 |
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| author | Haoran Xu Yuelin Bi Xin Feng Jiaqi Wang Gengyuan Yu Tonghua Zhang Tianyi Li Xuhua Gao Runhua Liu Yu Sun Hao Wu Linlin Fang Chenning Zhang Yikun Sun |
| author_facet | Haoran Xu Yuelin Bi Xin Feng Jiaqi Wang Gengyuan Yu Tonghua Zhang Tianyi Li Xuhua Gao Runhua Liu Yu Sun Hao Wu Linlin Fang Chenning Zhang Yikun Sun |
| author_sort | Haoran Xu |
| collection | DOAJ |
| description | The Dahuoluo pill (DHLP) is a classic Chinese patent medicine used to treat rheumatoid arthritis and other conditions. However, there has been no research on the chemical components of DHLP and the mechanisms by which it ameliorates rheumatoid arthritis. Hence, we analysed the chemical components of DHLP and the DHLP components absorbed in blood by using ultraperformance liquid chromatography-Q-exactive-orbitrap-mass spectrometry. We then used network pharmacology to predict the underlying mechanisms by which DHLP ameliorates rheumatoid arthritis. We identified 153 chemical compounds from DHLP, together with 27 prototype components absorbed in blood. We selected 48 of these compounds as potential active ingredients to explore the mechanism. These compounds are related to 88 significant pathways, which are linked to 18 core targets. This study preliminarily reveals the potential mechanisms by which DHLP ameliorates rheumatoid arthritis and provides a basis for further evaluation of the drug’s efficacy. |
| format | Article |
| id | doaj-art-1359308c794e4dca9e94246ab99fd98b |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-1359308c794e4dca9e94246ab99fd98b2025-02-03T06:43:08ZengWileyJournal of Analytical Methods in Chemistry2090-88732023-01-01202310.1155/2023/8819534Characterization of the Active Ingredient and Prediction of the Potential Mechanism of Dahuoluo Pill via Mass Spectrometry with the Network Pharmacology MethodHaoran Xu0Yuelin Bi1Xin Feng2Jiaqi Wang3Gengyuan Yu4Tonghua Zhang5Tianyi Li6Xuhua Gao7Runhua Liu8Yu Sun9Hao Wu10Linlin Fang11Chenning Zhang12Yikun Sun13School of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaCollege of PharmacySchool of Chinese Materia MedicaSchool of Chinese Materia MedicaThe Dahuoluo pill (DHLP) is a classic Chinese patent medicine used to treat rheumatoid arthritis and other conditions. However, there has been no research on the chemical components of DHLP and the mechanisms by which it ameliorates rheumatoid arthritis. Hence, we analysed the chemical components of DHLP and the DHLP components absorbed in blood by using ultraperformance liquid chromatography-Q-exactive-orbitrap-mass spectrometry. We then used network pharmacology to predict the underlying mechanisms by which DHLP ameliorates rheumatoid arthritis. We identified 153 chemical compounds from DHLP, together with 27 prototype components absorbed in blood. We selected 48 of these compounds as potential active ingredients to explore the mechanism. These compounds are related to 88 significant pathways, which are linked to 18 core targets. This study preliminarily reveals the potential mechanisms by which DHLP ameliorates rheumatoid arthritis and provides a basis for further evaluation of the drug’s efficacy.http://dx.doi.org/10.1155/2023/8819534 |
| spellingShingle | Haoran Xu Yuelin Bi Xin Feng Jiaqi Wang Gengyuan Yu Tonghua Zhang Tianyi Li Xuhua Gao Runhua Liu Yu Sun Hao Wu Linlin Fang Chenning Zhang Yikun Sun Characterization of the Active Ingredient and Prediction of the Potential Mechanism of Dahuoluo Pill via Mass Spectrometry with the Network Pharmacology Method Journal of Analytical Methods in Chemistry |
| title | Characterization of the Active Ingredient and Prediction of the Potential Mechanism of Dahuoluo Pill via Mass Spectrometry with the Network Pharmacology Method |
| title_full | Characterization of the Active Ingredient and Prediction of the Potential Mechanism of Dahuoluo Pill via Mass Spectrometry with the Network Pharmacology Method |
| title_fullStr | Characterization of the Active Ingredient and Prediction of the Potential Mechanism of Dahuoluo Pill via Mass Spectrometry with the Network Pharmacology Method |
| title_full_unstemmed | Characterization of the Active Ingredient and Prediction of the Potential Mechanism of Dahuoluo Pill via Mass Spectrometry with the Network Pharmacology Method |
| title_short | Characterization of the Active Ingredient and Prediction of the Potential Mechanism of Dahuoluo Pill via Mass Spectrometry with the Network Pharmacology Method |
| title_sort | characterization of the active ingredient and prediction of the potential mechanism of dahuoluo pill via mass spectrometry with the network pharmacology method |
| url | http://dx.doi.org/10.1155/2023/8819534 |
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