Veronicastrum axillare Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Suppression of Proinflammatory Mediators and Downregulation of the NF-κB Signaling Pathway
Veronicastrum axillare is a traditional medical plant in China which is widely used in folk medicine due to its versatile biological activities, especially for its anti-inflammatory effects. However, the detailed mechanism underlying this action is not clear. Here, we studied the protective effects...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/7934049 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558221062045696 |
|---|---|
| author | Quanxin Ma Kai Wang Qinqin Yang Shun Ping Weichun Zhao Qiyang Shou Weimin Zhou Minli Chen |
| author_facet | Quanxin Ma Kai Wang Qinqin Yang Shun Ping Weichun Zhao Qiyang Shou Weimin Zhou Minli Chen |
| author_sort | Quanxin Ma |
| collection | DOAJ |
| description | Veronicastrum axillare is a traditional medical plant in China which is widely used in folk medicine due to its versatile biological activities, especially for its anti-inflammatory effects. However, the detailed mechanism underlying this action is not clear. Here, we studied the protective effects of V. axillare against acute lung injury (ALI), and we further explored the pharmacological mechanisms of this action. We found that pretreatment with V. axillare suppressed the release of proinflammatory cytokines in the serum of ALI mice. Histological analysis of lung tissue demonstrated that V. axillare inhibited LPS-induced lung injury, improved lung morphology, and reduced the activation of nuclear factor-κB (NF-κB) in the lungs. Furthermore, the anti-inflammatory actions of V. axillare were investigated in vitro. We observed that V. axillare suppressed the mRNA expression of interleukin-1β (IL-1β), IL-6, monocyte chemotactic protein-1 (MCP-1), cyclooxygenase-2 (COX-2), and tumor necrosis factor-α (TNF-α) in RAW264.7 cells challenged with LPS. Furthermore, pretreatment of V. axillare in vitro reduced the phosphorylation of p65 and IκB-α which is activated by LPS. In conclusion, our data firstly demonstrated that the anti-inflammatory effects of V. axillare against ALI were achieved through downregulation of the NF-κB signaling pathway, thereby reducing the production of inflammatory mediators. |
| format | Article |
| id | doaj-art-1358706f0ea34f7d8b036db5b7188c24 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1358706f0ea34f7d8b036db5b7188c242025-02-03T01:32:53ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/79340497934049Veronicastrum axillare Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Suppression of Proinflammatory Mediators and Downregulation of the NF-κB Signaling PathwayQuanxin Ma0Kai Wang1Qinqin Yang2Shun Ping3Weichun Zhao4Qiyang Shou5Weimin Zhou6Minli Chen7Animal Experimental Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaZhejiang Institute of Traditional Chinese Medicine, Hangzhou 310007, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaCollege of Bioengineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaAnimal Experimental Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaAnimal Experimental Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaAnimal Experimental Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaVeronicastrum axillare is a traditional medical plant in China which is widely used in folk medicine due to its versatile biological activities, especially for its anti-inflammatory effects. However, the detailed mechanism underlying this action is not clear. Here, we studied the protective effects of V. axillare against acute lung injury (ALI), and we further explored the pharmacological mechanisms of this action. We found that pretreatment with V. axillare suppressed the release of proinflammatory cytokines in the serum of ALI mice. Histological analysis of lung tissue demonstrated that V. axillare inhibited LPS-induced lung injury, improved lung morphology, and reduced the activation of nuclear factor-κB (NF-κB) in the lungs. Furthermore, the anti-inflammatory actions of V. axillare were investigated in vitro. We observed that V. axillare suppressed the mRNA expression of interleukin-1β (IL-1β), IL-6, monocyte chemotactic protein-1 (MCP-1), cyclooxygenase-2 (COX-2), and tumor necrosis factor-α (TNF-α) in RAW264.7 cells challenged with LPS. Furthermore, pretreatment of V. axillare in vitro reduced the phosphorylation of p65 and IκB-α which is activated by LPS. In conclusion, our data firstly demonstrated that the anti-inflammatory effects of V. axillare against ALI were achieved through downregulation of the NF-κB signaling pathway, thereby reducing the production of inflammatory mediators.http://dx.doi.org/10.1155/2016/7934049 |
| spellingShingle | Quanxin Ma Kai Wang Qinqin Yang Shun Ping Weichun Zhao Qiyang Shou Weimin Zhou Minli Chen Veronicastrum axillare Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Suppression of Proinflammatory Mediators and Downregulation of the NF-κB Signaling Pathway Mediators of Inflammation |
| title | Veronicastrum axillare Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Suppression of Proinflammatory Mediators and Downregulation of the NF-κB Signaling Pathway |
| title_full | Veronicastrum axillare Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Suppression of Proinflammatory Mediators and Downregulation of the NF-κB Signaling Pathway |
| title_fullStr | Veronicastrum axillare Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Suppression of Proinflammatory Mediators and Downregulation of the NF-κB Signaling Pathway |
| title_full_unstemmed | Veronicastrum axillare Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Suppression of Proinflammatory Mediators and Downregulation of the NF-κB Signaling Pathway |
| title_short | Veronicastrum axillare Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Suppression of Proinflammatory Mediators and Downregulation of the NF-κB Signaling Pathway |
| title_sort | veronicastrum axillare alleviates lipopolysaccharide induced acute lung injury via suppression of proinflammatory mediators and downregulation of the nf κb signaling pathway |
| url | http://dx.doi.org/10.1155/2016/7934049 |
| work_keys_str_mv | AT quanxinma veronicastrumaxillarealleviateslipopolysaccharideinducedacutelunginjuryviasuppressionofproinflammatorymediatorsanddownregulationofthenfkbsignalingpathway AT kaiwang veronicastrumaxillarealleviateslipopolysaccharideinducedacutelunginjuryviasuppressionofproinflammatorymediatorsanddownregulationofthenfkbsignalingpathway AT qinqinyang veronicastrumaxillarealleviateslipopolysaccharideinducedacutelunginjuryviasuppressionofproinflammatorymediatorsanddownregulationofthenfkbsignalingpathway AT shunping veronicastrumaxillarealleviateslipopolysaccharideinducedacutelunginjuryviasuppressionofproinflammatorymediatorsanddownregulationofthenfkbsignalingpathway AT weichunzhao veronicastrumaxillarealleviateslipopolysaccharideinducedacutelunginjuryviasuppressionofproinflammatorymediatorsanddownregulationofthenfkbsignalingpathway AT qiyangshou veronicastrumaxillarealleviateslipopolysaccharideinducedacutelunginjuryviasuppressionofproinflammatorymediatorsanddownregulationofthenfkbsignalingpathway AT weiminzhou veronicastrumaxillarealleviateslipopolysaccharideinducedacutelunginjuryviasuppressionofproinflammatorymediatorsanddownregulationofthenfkbsignalingpathway AT minlichen veronicastrumaxillarealleviateslipopolysaccharideinducedacutelunginjuryviasuppressionofproinflammatorymediatorsanddownregulationofthenfkbsignalingpathway |