Rapid, Simple, and Sensitive Spectrofluorimetric Method for the Estimation of Ganciclovir in Bulk and Pharmaceutical Formulations
A new, simple, rapid, sensitive, accurate, and affordable spectrofluorimetric method was developed and validated for the estimation of ganciclovir in bulk as well as in marketed formulations. The method was based on measuring the native fluorescence of ganciclovir in 0.2 M hydrochloric acid buffer o...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2013/972806 |
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| author | Garima Balwani Emil Joseph Satish Reddi Vibhu Nagpal Ranendra N. Saha |
| author_facet | Garima Balwani Emil Joseph Satish Reddi Vibhu Nagpal Ranendra N. Saha |
| author_sort | Garima Balwani |
| collection | DOAJ |
| description | A new, simple, rapid, sensitive, accurate, and affordable spectrofluorimetric method was developed and validated for the estimation of ganciclovir in bulk as well as in marketed formulations. The method was based on measuring the native fluorescence of ganciclovir in 0.2 M hydrochloric acid buffer of pH 1.2 at 374 nm after excitation at 257 nm. The calibration graph was found to be rectilinear in the concentration range of 0.25–2.00 μg mL−1. The limit of quantification and limit of detection were found to be 0.029 μg mL−1 and 0.010 μg mL−1, respectively. The method was fully validated for various parameters according to ICH guidelines. The results demonstrated that the procedure is accurate, precise, and reproducible (relative standard deviation <2%) and can be successfully applied for the determination of ganciclovir in its commercial capsules with average percentage recovery of 101.31 ± 0.90. |
| format | Article |
| id | doaj-art-1358558612c74aa4b26623ab9cc9a4b9 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-1358558612c74aa4b26623ab9cc9a4b92025-02-03T01:04:45ZengWileyJournal of Spectroscopy2314-49202314-49392013-01-01201310.1155/2013/972806972806Rapid, Simple, and Sensitive Spectrofluorimetric Method for the Estimation of Ganciclovir in Bulk and Pharmaceutical FormulationsGarima Balwani0Emil Joseph1Satish Reddi2Vibhu Nagpal3Ranendra N. Saha4Department of Pharmacy, BITS Pilani, Pilani Campus, Rajasthan 333031, IndiaDepartment of Pharmacy, BITS Pilani, Pilani Campus, Rajasthan 333031, IndiaDepartment of Pharmacy, BITS Pilani, Pilani Campus, Rajasthan 333031, IndiaDepartment of Pharmacy, BITS Pilani, Pilani Campus, Rajasthan 333031, IndiaDepartment of Pharmacy, BITS Pilani, Pilani Campus, Rajasthan 333031, IndiaA new, simple, rapid, sensitive, accurate, and affordable spectrofluorimetric method was developed and validated for the estimation of ganciclovir in bulk as well as in marketed formulations. The method was based on measuring the native fluorescence of ganciclovir in 0.2 M hydrochloric acid buffer of pH 1.2 at 374 nm after excitation at 257 nm. The calibration graph was found to be rectilinear in the concentration range of 0.25–2.00 μg mL−1. The limit of quantification and limit of detection were found to be 0.029 μg mL−1 and 0.010 μg mL−1, respectively. The method was fully validated for various parameters according to ICH guidelines. The results demonstrated that the procedure is accurate, precise, and reproducible (relative standard deviation <2%) and can be successfully applied for the determination of ganciclovir in its commercial capsules with average percentage recovery of 101.31 ± 0.90.http://dx.doi.org/10.1155/2013/972806 |
| spellingShingle | Garima Balwani Emil Joseph Satish Reddi Vibhu Nagpal Ranendra N. Saha Rapid, Simple, and Sensitive Spectrofluorimetric Method for the Estimation of Ganciclovir in Bulk and Pharmaceutical Formulations Journal of Spectroscopy |
| title | Rapid, Simple, and Sensitive Spectrofluorimetric Method for the Estimation of Ganciclovir in Bulk and Pharmaceutical Formulations |
| title_full | Rapid, Simple, and Sensitive Spectrofluorimetric Method for the Estimation of Ganciclovir in Bulk and Pharmaceutical Formulations |
| title_fullStr | Rapid, Simple, and Sensitive Spectrofluorimetric Method for the Estimation of Ganciclovir in Bulk and Pharmaceutical Formulations |
| title_full_unstemmed | Rapid, Simple, and Sensitive Spectrofluorimetric Method for the Estimation of Ganciclovir in Bulk and Pharmaceutical Formulations |
| title_short | Rapid, Simple, and Sensitive Spectrofluorimetric Method for the Estimation of Ganciclovir in Bulk and Pharmaceutical Formulations |
| title_sort | rapid simple and sensitive spectrofluorimetric method for the estimation of ganciclovir in bulk and pharmaceutical formulations |
| url | http://dx.doi.org/10.1155/2013/972806 |
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