Effect of Antifungal-Treated Host Macrophages on Candida glabrata
Objective. Candida glabrata (C. glabrata) causes infections associated with severe sepsis and high mortality. This study describes the effects of micafungin (MCF), itraconazole (ICZ), and amphotericin B (AmB) on the function of macrophages during C. glabrata infection. Methods. RAW264.1 macrophages...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/8838444 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832552754362449920 |
|---|---|
| author | Hong-Bin Li Na Li Shu-Ran Wen Ming-Yue Qiang Zheng-Hui Yang Tian-Xiang Dong Yu-Ye Li Yi-Qun Kuang |
| author_facet | Hong-Bin Li Na Li Shu-Ran Wen Ming-Yue Qiang Zheng-Hui Yang Tian-Xiang Dong Yu-Ye Li Yi-Qun Kuang |
| author_sort | Hong-Bin Li |
| collection | DOAJ |
| description | Objective. Candida glabrata (C. glabrata) causes infections associated with severe sepsis and high mortality. This study describes the effects of micafungin (MCF), itraconazole (ICZ), and amphotericin B (AmB) on the function of macrophages during C. glabrata infection. Methods. RAW264.1 macrophages were treated with MCF, ICZ, or AmB and then challenged with C. glabrata. Cytokines from infected macrophage supernatants and the levels of superoxide dismutase (SOD) in macrophages were measured at different time points after phagocytosis. Results. The activity of SOD was significantly increased in RAW264.1 cells that phagocytized C. glabrata and reached a peak level at 6 hours (P<0.05). ICZ and AmB did not affect the SOD activity in cells that phagocytized C. glabrata versus that in untreated macrophage. C. glabrata stimulated macrophages to secrete cytokines. Neither ICZ nor AmB affected the secretion of interleukin-6 (IL-6), interleukin-8 (IL-8), or tumor necrosis factor-α (TNF-α) by C. glabrata-infected macrophages. However, MCF downregulated the secretion of TNF-α by infected macrophages and reduced the SOD activity of C. glabrata compared with those in untreated controls. Conclusion. Echinocandins may increase their antifungal efficacy by altering the innate immune response of macrophages and attenuating antioxidants of this organism. |
| format | Article |
| id | doaj-art-135489bee4164ae1b7c82c76b0819be3 |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-135489bee4164ae1b7c82c76b0819be32025-02-03T05:57:50ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932021-01-01202110.1155/2021/88384448838444Effect of Antifungal-Treated Host Macrophages on Candida glabrataHong-Bin Li0Na Li1Shu-Ran Wen2Ming-Yue Qiang3Zheng-Hui Yang4Tian-Xiang Dong5Yu-Ye Li6Yi-Qun Kuang7Department of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, ChinaDepartment of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, ChinaDepartment of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, ChinaDepartment of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, ChinaDepartment of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, ChinaDepartment of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, ChinaDepartment of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, ChinaNHC Key Laboratory of Drug Addiction Medicine, First Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming 650032, Yunnan, ChinaObjective. Candida glabrata (C. glabrata) causes infections associated with severe sepsis and high mortality. This study describes the effects of micafungin (MCF), itraconazole (ICZ), and amphotericin B (AmB) on the function of macrophages during C. glabrata infection. Methods. RAW264.1 macrophages were treated with MCF, ICZ, or AmB and then challenged with C. glabrata. Cytokines from infected macrophage supernatants and the levels of superoxide dismutase (SOD) in macrophages were measured at different time points after phagocytosis. Results. The activity of SOD was significantly increased in RAW264.1 cells that phagocytized C. glabrata and reached a peak level at 6 hours (P<0.05). ICZ and AmB did not affect the SOD activity in cells that phagocytized C. glabrata versus that in untreated macrophage. C. glabrata stimulated macrophages to secrete cytokines. Neither ICZ nor AmB affected the secretion of interleukin-6 (IL-6), interleukin-8 (IL-8), or tumor necrosis factor-α (TNF-α) by C. glabrata-infected macrophages. However, MCF downregulated the secretion of TNF-α by infected macrophages and reduced the SOD activity of C. glabrata compared with those in untreated controls. Conclusion. Echinocandins may increase their antifungal efficacy by altering the innate immune response of macrophages and attenuating antioxidants of this organism.http://dx.doi.org/10.1155/2021/8838444 |
| spellingShingle | Hong-Bin Li Na Li Shu-Ran Wen Ming-Yue Qiang Zheng-Hui Yang Tian-Xiang Dong Yu-Ye Li Yi-Qun Kuang Effect of Antifungal-Treated Host Macrophages on Candida glabrata Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Effect of Antifungal-Treated Host Macrophages on Candida glabrata |
| title_full | Effect of Antifungal-Treated Host Macrophages on Candida glabrata |
| title_fullStr | Effect of Antifungal-Treated Host Macrophages on Candida glabrata |
| title_full_unstemmed | Effect of Antifungal-Treated Host Macrophages on Candida glabrata |
| title_short | Effect of Antifungal-Treated Host Macrophages on Candida glabrata |
| title_sort | effect of antifungal treated host macrophages on candida glabrata |
| url | http://dx.doi.org/10.1155/2021/8838444 |
| work_keys_str_mv | AT hongbinli effectofantifungaltreatedhostmacrophagesoncandidaglabrata AT nali effectofantifungaltreatedhostmacrophagesoncandidaglabrata AT shuranwen effectofantifungaltreatedhostmacrophagesoncandidaglabrata AT mingyueqiang effectofantifungaltreatedhostmacrophagesoncandidaglabrata AT zhenghuiyang effectofantifungaltreatedhostmacrophagesoncandidaglabrata AT tianxiangdong effectofantifungaltreatedhostmacrophagesoncandidaglabrata AT yuyeli effectofantifungaltreatedhostmacrophagesoncandidaglabrata AT yiqunkuang effectofantifungaltreatedhostmacrophagesoncandidaglabrata |