Secoxyloganin inhibits growth of breast cancer MDA-MB-231 cells via induction of apoptosis and cell cycle arrest
This study was set to study the anti-proliferative effects of secoxyloganin in MDA-MB-231 breast cancer cells. The MTT cell viability assay results demonstrated that secoxyloganin significantly (p < 0.05) inhibited the growth of the MDA-MB-231 cells with an IC50 of 6.5 μM which was significantly...
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Taylor & Francis Group
2024-12-01
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| Online Access: | http://dx.doi.org/10.1080/26895293.2024.2408433 |
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| author | Jie Ma Cong Peng |
| author_facet | Jie Ma Cong Peng |
| author_sort | Jie Ma |
| collection | DOAJ |
| description | This study was set to study the anti-proliferative effects of secoxyloganin in MDA-MB-231 breast cancer cells. The MTT cell viability assay results demonstrated that secoxyloganin significantly (p < 0.05) inhibited the growth of the MDA-MB-231 cells with an IC50 of 6.5 μM which was significantly (p < 0.05) lower than the IC50 of 38 μM against the normal fR2 cells. Unveiling of molecular mechanism revealed that secoxyloganin disrupted the DNA integrity of MDA-MB-231 cells and triggered apoptosis. Annexin V/PI staining revealed that the percentage of apoptotic MDA-MB-231 cells increased from 3.7% in control to 40.2% at 24 μM of secoxyloganin. This was accompanied by an enhancement of Bax, and suppression of Bcl-2. The effects of secoxyloganin on the distribution of cells in various cell cycle phases was also assessed. The results showed the proportion of cells in the G0/G1 phase increased from 32.76% in control to 77.50% at 24 μM of secoxyloganin suggesting G0/G1 cell cycle arrest. This was also associated with suppression of cyclin D2 expression. Taken together, these findings demonstrate the anticancer properties of secoxyloganin through cell cycle arrest and apoptosis. |
| format | Article |
| id | doaj-art-132b018394184a4a85e9cda52a399a24 |
| institution | Kabale University |
| issn | 2689-5307 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-132b018394184a4a85e9cda52a399a242025-01-20T14:38:00ZengTaylor & Francis GroupAll Life2689-53072024-12-01170110.1080/26895293.2024.24084332408433Secoxyloganin inhibits growth of breast cancer MDA-MB-231 cells via induction of apoptosis and cell cycle arrestJie Ma0Cong Peng1Jingzhou Central Hospital, Jingzhou Hospital, Yangtze UniversityJingzhou Central Hospital, Jingzhou Hospital, Yangtze UniversityThis study was set to study the anti-proliferative effects of secoxyloganin in MDA-MB-231 breast cancer cells. The MTT cell viability assay results demonstrated that secoxyloganin significantly (p < 0.05) inhibited the growth of the MDA-MB-231 cells with an IC50 of 6.5 μM which was significantly (p < 0.05) lower than the IC50 of 38 μM against the normal fR2 cells. Unveiling of molecular mechanism revealed that secoxyloganin disrupted the DNA integrity of MDA-MB-231 cells and triggered apoptosis. Annexin V/PI staining revealed that the percentage of apoptotic MDA-MB-231 cells increased from 3.7% in control to 40.2% at 24 μM of secoxyloganin. This was accompanied by an enhancement of Bax, and suppression of Bcl-2. The effects of secoxyloganin on the distribution of cells in various cell cycle phases was also assessed. The results showed the proportion of cells in the G0/G1 phase increased from 32.76% in control to 77.50% at 24 μM of secoxyloganin suggesting G0/G1 cell cycle arrest. This was also associated with suppression of cyclin D2 expression. Taken together, these findings demonstrate the anticancer properties of secoxyloganin through cell cycle arrest and apoptosis.http://dx.doi.org/10.1080/26895293.2024.2408433breast cancernatural productsiridoid glycosidessecoxyloganinapoptosiscell cycle |
| spellingShingle | Jie Ma Cong Peng Secoxyloganin inhibits growth of breast cancer MDA-MB-231 cells via induction of apoptosis and cell cycle arrest All Life breast cancer natural products iridoid glycosides secoxyloganin apoptosis cell cycle |
| title | Secoxyloganin inhibits growth of breast cancer MDA-MB-231 cells via induction of apoptosis and cell cycle arrest |
| title_full | Secoxyloganin inhibits growth of breast cancer MDA-MB-231 cells via induction of apoptosis and cell cycle arrest |
| title_fullStr | Secoxyloganin inhibits growth of breast cancer MDA-MB-231 cells via induction of apoptosis and cell cycle arrest |
| title_full_unstemmed | Secoxyloganin inhibits growth of breast cancer MDA-MB-231 cells via induction of apoptosis and cell cycle arrest |
| title_short | Secoxyloganin inhibits growth of breast cancer MDA-MB-231 cells via induction of apoptosis and cell cycle arrest |
| title_sort | secoxyloganin inhibits growth of breast cancer mda mb 231 cells via induction of apoptosis and cell cycle arrest |
| topic | breast cancer natural products iridoid glycosides secoxyloganin apoptosis cell cycle |
| url | http://dx.doi.org/10.1080/26895293.2024.2408433 |
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