Secoxyloganin inhibits growth of breast cancer MDA-MB-231 cells via induction of apoptosis and cell cycle arrest
This study was set to study the anti-proliferative effects of secoxyloganin in MDA-MB-231 breast cancer cells. The MTT cell viability assay results demonstrated that secoxyloganin significantly (p < 0.05) inhibited the growth of the MDA-MB-231 cells with an IC50 of 6.5 μM which was significantly...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
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| Series: | All Life |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/26895293.2024.2408433 |
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| Summary: | This study was set to study the anti-proliferative effects of secoxyloganin in MDA-MB-231 breast cancer cells. The MTT cell viability assay results demonstrated that secoxyloganin significantly (p < 0.05) inhibited the growth of the MDA-MB-231 cells with an IC50 of 6.5 μM which was significantly (p < 0.05) lower than the IC50 of 38 μM against the normal fR2 cells. Unveiling of molecular mechanism revealed that secoxyloganin disrupted the DNA integrity of MDA-MB-231 cells and triggered apoptosis. Annexin V/PI staining revealed that the percentage of apoptotic MDA-MB-231 cells increased from 3.7% in control to 40.2% at 24 μM of secoxyloganin. This was accompanied by an enhancement of Bax, and suppression of Bcl-2. The effects of secoxyloganin on the distribution of cells in various cell cycle phases was also assessed. The results showed the proportion of cells in the G0/G1 phase increased from 32.76% in control to 77.50% at 24 μM of secoxyloganin suggesting G0/G1 cell cycle arrest. This was also associated with suppression of cyclin D2 expression. Taken together, these findings demonstrate the anticancer properties of secoxyloganin through cell cycle arrest and apoptosis. |
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| ISSN: | 2689-5307 |