Interleukin-32γ in the Control of Acute Experimental Chagas Disease
Chagas disease (CD) is an important parasitic disease caused by Trypanosoma cruzi. Interleukin-32 (IL-32) plays an important role in inflammation and in the development of Th1/Th17 acquired immune responses. We evaluated the influence of IL-32γ on the immune response profile, pathogenesis of myocard...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/7070301 |
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| author | Yarlla L. L. Braga José R. C. Neto Arthur W. F. Costa Muriel V. T. Silva Marcos V. Silva Mara R. N. Celes Milton A. P. Oliveira Leo A. B. Joosten Fátima Ribeiro-Dias Rodrigo S. Gomes Juliana R. Machado |
| author_facet | Yarlla L. L. Braga José R. C. Neto Arthur W. F. Costa Muriel V. T. Silva Marcos V. Silva Mara R. N. Celes Milton A. P. Oliveira Leo A. B. Joosten Fátima Ribeiro-Dias Rodrigo S. Gomes Juliana R. Machado |
| author_sort | Yarlla L. L. Braga |
| collection | DOAJ |
| description | Chagas disease (CD) is an important parasitic disease caused by Trypanosoma cruzi. Interleukin-32 (IL-32) plays an important role in inflammation and in the development of Th1/Th17 acquired immune responses. We evaluated the influence of IL-32γ on the immune response profile, pathogenesis of myocarditis in acute experimental CD, and control of the disease. For this, C57BL/6 wild-type (WT) and IL-32γTg mice were infected subcutaneously with 1,000 forms of Colombian strain of T. cruzi. In the histopathological analyzes, T. cruzi nests, myocarditis, and collagen were quantified in cardiac tissue. Cytokine productions (IL-32, IFN-γ, TNF-α, IL-10, and IL-17) were measured in cardiac homogenate by ELISA. The IL-32γTg mice showed a better control of parasitemia and T. cruzi nests in the heart than WT mice. Infected-WT and -IL-32γTg mice showed similar levels of IFN-γ, TNF-α, and IL-17, but IL-10 was significantly higher expressed in IL-32γTg than in WT mice. The cytokine profile found in IL-32γTg animals contributed to body weight maintenance, parasitemia control, and survival. Our results indicate that the presence of human IL-32γ in mice infected with the Colombian strain of T. cruzi is important for infection control during the acute phase of Chagas disease. |
| format | Article |
| id | doaj-art-1326e035a75e4c538df45451860b1786 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-1326e035a75e4c538df45451860b17862025-02-03T06:11:19ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/7070301Interleukin-32γ in the Control of Acute Experimental Chagas DiseaseYarlla L. L. Braga0José R. C. Neto1Arthur W. F. Costa2Muriel V. T. Silva3Marcos V. Silva4Mara R. N. Celes5Milton A. P. Oliveira6Leo A. B. Joosten7Fátima Ribeiro-Dias8Rodrigo S. Gomes9Juliana R. Machado10Instituto de Patologia Tropical e Saúde PúblicaInstituto de Patologia Tropical e Saúde PúblicaInstituto de Patologia Tropical e Saúde PúblicaInstituto de Patologia Tropical e Saúde PúblicaDepartamento de MicrobiologiaInstituto de Patologia Tropical e Saúde PúblicaInstituto de Patologia Tropical e Saúde PúblicaInstituto de Patologia Tropical e Saúde PúblicaInstituto de Patologia Tropical e Saúde PúblicaInstituto de Patologia Tropical e Saúde PúblicaInstituto de Patologia Tropical e Saúde PúblicaChagas disease (CD) is an important parasitic disease caused by Trypanosoma cruzi. Interleukin-32 (IL-32) plays an important role in inflammation and in the development of Th1/Th17 acquired immune responses. We evaluated the influence of IL-32γ on the immune response profile, pathogenesis of myocarditis in acute experimental CD, and control of the disease. For this, C57BL/6 wild-type (WT) and IL-32γTg mice were infected subcutaneously with 1,000 forms of Colombian strain of T. cruzi. In the histopathological analyzes, T. cruzi nests, myocarditis, and collagen were quantified in cardiac tissue. Cytokine productions (IL-32, IFN-γ, TNF-α, IL-10, and IL-17) were measured in cardiac homogenate by ELISA. The IL-32γTg mice showed a better control of parasitemia and T. cruzi nests in the heart than WT mice. Infected-WT and -IL-32γTg mice showed similar levels of IFN-γ, TNF-α, and IL-17, but IL-10 was significantly higher expressed in IL-32γTg than in WT mice. The cytokine profile found in IL-32γTg animals contributed to body weight maintenance, parasitemia control, and survival. Our results indicate that the presence of human IL-32γ in mice infected with the Colombian strain of T. cruzi is important for infection control during the acute phase of Chagas disease.http://dx.doi.org/10.1155/2022/7070301 |
| spellingShingle | Yarlla L. L. Braga José R. C. Neto Arthur W. F. Costa Muriel V. T. Silva Marcos V. Silva Mara R. N. Celes Milton A. P. Oliveira Leo A. B. Joosten Fátima Ribeiro-Dias Rodrigo S. Gomes Juliana R. Machado Interleukin-32γ in the Control of Acute Experimental Chagas Disease Journal of Immunology Research |
| title | Interleukin-32γ in the Control of Acute Experimental Chagas Disease |
| title_full | Interleukin-32γ in the Control of Acute Experimental Chagas Disease |
| title_fullStr | Interleukin-32γ in the Control of Acute Experimental Chagas Disease |
| title_full_unstemmed | Interleukin-32γ in the Control of Acute Experimental Chagas Disease |
| title_short | Interleukin-32γ in the Control of Acute Experimental Chagas Disease |
| title_sort | interleukin 32γ in the control of acute experimental chagas disease |
| url | http://dx.doi.org/10.1155/2022/7070301 |
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