Comparative analysis of Oreochromis niloticus responses to Chlorella vulgaris and Lactobacillus-fermented Azolla economic feed supplements in biofloc and green systems: An in silico evaluation of metabolic and physiological responses
The field of high-effective functional foods has emerged from advancements in biological pharmaceutical research, bridging pharmacology and food science. Microorganisms have been used in food production for ages. In this study, two lactobacilli were used for Azolla fermentation to be used as an econ...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006847 |
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| author | Ahmed M. Aboseif Nasser S. Flefil Mostafa K.S. Taha Asmaa Negm El-Dein Mingchun Ren Hualiang Liang Abd-Ellatif M. Hussian |
| author_facet | Ahmed M. Aboseif Nasser S. Flefil Mostafa K.S. Taha Asmaa Negm El-Dein Mingchun Ren Hualiang Liang Abd-Ellatif M. Hussian |
| author_sort | Ahmed M. Aboseif |
| collection | DOAJ |
| description | The field of high-effective functional foods has emerged from advancements in biological pharmaceutical research, bridging pharmacology and food science. Microorganisms have been used in food production for ages. In this study, two lactobacilli were used for Azolla fermentation to be used as an economic aquafeed ingredient to identify the most suitable condition in tilapia rearing systems. The study explored the impact of using fermented Azolla and Chlorella vulgaris as a fish feed component in aquaculture systems, emphasizing their nutritional and environmental benefits. The fermentation process, involving Lactobacillus plantarum KU985433 and L. rhamnosus KU985437, enhances Azolla’s protein, phenolic, and antioxidant content while reducing carbohydrates and lipids. Comparative trials in biofloc and green water systems showed that fish fed with fermented Azolla exhibited improved growth, feed utilization, and immune response, demonstrating the potential of fermented plant-based feed as a sustainable alternative to conventional soybean meal. In silico analyses using PCA, heat maps, and network analysis identified that the optimal feed conditions were achieved using 50 % soy bean substitution with Azolla fermented with L. plantarum in both systems, highlighting the efficacy of incorporating fermented Azolla in aquaculture. |
| format | Article |
| id | doaj-art-12f766e33bd24b07930b29bcd716d723 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-12f766e33bd24b07930b29bcd716d7232025-02-06T05:12:15ZengElsevierAquaculture Reports2352-51342025-03-0140102596Comparative analysis of Oreochromis niloticus responses to Chlorella vulgaris and Lactobacillus-fermented Azolla economic feed supplements in biofloc and green systems: An in silico evaluation of metabolic and physiological responsesAhmed M. Aboseif0Nasser S. Flefil1Mostafa K.S. Taha2Asmaa Negm El-Dein3Mingchun Ren4Hualiang Liang5Abd-Ellatif M. Hussian6National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt; Academy of Scientific Research and Technology (ASRT), Cairo, EgyptNational Institute of Oceanography and Fisheries, NIOF, Cairo, EgyptNational Institute of Oceanography and Fisheries, NIOF, Cairo, EgyptChemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Giza, EgyptKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Corresponding authors.Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Corresponding authors.Matrouh University, Faculty of Education, Marsa Matrouh, EgyptThe field of high-effective functional foods has emerged from advancements in biological pharmaceutical research, bridging pharmacology and food science. Microorganisms have been used in food production for ages. In this study, two lactobacilli were used for Azolla fermentation to be used as an economic aquafeed ingredient to identify the most suitable condition in tilapia rearing systems. The study explored the impact of using fermented Azolla and Chlorella vulgaris as a fish feed component in aquaculture systems, emphasizing their nutritional and environmental benefits. The fermentation process, involving Lactobacillus plantarum KU985433 and L. rhamnosus KU985437, enhances Azolla’s protein, phenolic, and antioxidant content while reducing carbohydrates and lipids. Comparative trials in biofloc and green water systems showed that fish fed with fermented Azolla exhibited improved growth, feed utilization, and immune response, demonstrating the potential of fermented plant-based feed as a sustainable alternative to conventional soybean meal. In silico analyses using PCA, heat maps, and network analysis identified that the optimal feed conditions were achieved using 50 % soy bean substitution with Azolla fermented with L. plantarum in both systems, highlighting the efficacy of incorporating fermented Azolla in aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513424006847Oreochromis niloticusLactobacillus-fermented AzollaChlorella vulgarisBiofloc systemIn silico evaluation |
| spellingShingle | Ahmed M. Aboseif Nasser S. Flefil Mostafa K.S. Taha Asmaa Negm El-Dein Mingchun Ren Hualiang Liang Abd-Ellatif M. Hussian Comparative analysis of Oreochromis niloticus responses to Chlorella vulgaris and Lactobacillus-fermented Azolla economic feed supplements in biofloc and green systems: An in silico evaluation of metabolic and physiological responses Aquaculture Reports Oreochromis niloticus Lactobacillus-fermented Azolla Chlorella vulgaris Biofloc system In silico evaluation |
| title | Comparative analysis of Oreochromis niloticus responses to Chlorella vulgaris and Lactobacillus-fermented Azolla economic feed supplements in biofloc and green systems: An in silico evaluation of metabolic and physiological responses |
| title_full | Comparative analysis of Oreochromis niloticus responses to Chlorella vulgaris and Lactobacillus-fermented Azolla economic feed supplements in biofloc and green systems: An in silico evaluation of metabolic and physiological responses |
| title_fullStr | Comparative analysis of Oreochromis niloticus responses to Chlorella vulgaris and Lactobacillus-fermented Azolla economic feed supplements in biofloc and green systems: An in silico evaluation of metabolic and physiological responses |
| title_full_unstemmed | Comparative analysis of Oreochromis niloticus responses to Chlorella vulgaris and Lactobacillus-fermented Azolla economic feed supplements in biofloc and green systems: An in silico evaluation of metabolic and physiological responses |
| title_short | Comparative analysis of Oreochromis niloticus responses to Chlorella vulgaris and Lactobacillus-fermented Azolla economic feed supplements in biofloc and green systems: An in silico evaluation of metabolic and physiological responses |
| title_sort | comparative analysis of oreochromis niloticus responses to chlorella vulgaris and lactobacillus fermented azolla economic feed supplements in biofloc and green systems an in silico evaluation of metabolic and physiological responses |
| topic | Oreochromis niloticus Lactobacillus-fermented Azolla Chlorella vulgaris Biofloc system In silico evaluation |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006847 |
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