The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells
Background. C-reactive protein (CRP) is an independent biomarker of systemic inflammation and a predictor of future cardiovascular disease (CVD). More than just a pure bystander, CRP directly interacts with endothelial cells to decrease endothelial nitric oxide synthase (eNOS) expression and bioacti...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/6687250 |
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| author | Chenyi Ling Marc D. Cook Heather Grimm Maitha Aldokhayyil Dulce Gomez Michael Brown |
| author_facet | Chenyi Ling Marc D. Cook Heather Grimm Maitha Aldokhayyil Dulce Gomez Michael Brown |
| author_sort | Chenyi Ling |
| collection | DOAJ |
| description | Background. C-reactive protein (CRP) is an independent biomarker of systemic inflammation and a predictor of future cardiovascular disease (CVD). More than just a pure bystander, CRP directly interacts with endothelial cells to decrease endothelial nitric oxide synthase (eNOS) expression and bioactivity, decrease nitric oxide (NO) production, and increase the release of vasoconstrictors and adhesion molecules. Race is significantly associated with CRP levels and CVD risks. With aerobic exercise, the vessel wall is exposed to chronic high laminar shear stress (HiLSS) that shifts the endothelium phenotype towards an anti-inflammatory, antioxidant, antiapoptotic, and antiproliferative environment. Thus, the purpose of this study was to assess the racial differences concerning the CRP-induced effects in endothelial cells and the potential role of HiLSS in mitigating these differences. Methods. Human umbilical vein endothelial cells (HUVECs) from four African American (AA) and four Caucasian (CA) donors were cultured and incubated under the following conditions: (1) static control, (2) CRP (10 μg/mL, 24 hours), (3) CRP receptor (FcγRIIB) inhibitor followed by CRP stimulation, (4) HiLSS (20 dyne/cm2, 24 hours), and (5) HiLSS followed by CRP stimulation. Results. AA HUVECs had significantly higher FcγRIIB receptor expression under both basal and CRP incubation conditions. Blocking FcγRIIB receptor significantly attenuated the CRP-induced decrements in eNOS expression only in AA HUVECs. Finally, HiLSS significantly counteracted CRP-induced effects. Conclusion. Understanding potential racial differences in endothelial function is important to improve CVD prevention. Our results shed light on FcγRIIB receptor as a potential contributor to racial differences in endothelial function in AA. |
| format | Article |
| id | doaj-art-12dfd8b053434e028e15f23fb333f15d |
| institution | Kabale University |
| issn | 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-12dfd8b053434e028e15f23fb333f15d2025-02-03T05:46:37ZengWileyMediators of Inflammation1466-18612021-01-01202110.1155/2021/6687250The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial CellsChenyi Ling0Marc D. Cook1Heather Grimm2Maitha Aldokhayyil3Dulce Gomez4Michael Brown5Department of Kinesiology & NutritionDepartment of Kinesiology & NutritionDepartment of Kinesiology & NutritionDepartment of Kinesiology & NutritionSchool of KinesiologyDepartment of Kinesiology & NutritionBackground. C-reactive protein (CRP) is an independent biomarker of systemic inflammation and a predictor of future cardiovascular disease (CVD). More than just a pure bystander, CRP directly interacts with endothelial cells to decrease endothelial nitric oxide synthase (eNOS) expression and bioactivity, decrease nitric oxide (NO) production, and increase the release of vasoconstrictors and adhesion molecules. Race is significantly associated with CRP levels and CVD risks. With aerobic exercise, the vessel wall is exposed to chronic high laminar shear stress (HiLSS) that shifts the endothelium phenotype towards an anti-inflammatory, antioxidant, antiapoptotic, and antiproliferative environment. Thus, the purpose of this study was to assess the racial differences concerning the CRP-induced effects in endothelial cells and the potential role of HiLSS in mitigating these differences. Methods. Human umbilical vein endothelial cells (HUVECs) from four African American (AA) and four Caucasian (CA) donors were cultured and incubated under the following conditions: (1) static control, (2) CRP (10 μg/mL, 24 hours), (3) CRP receptor (FcγRIIB) inhibitor followed by CRP stimulation, (4) HiLSS (20 dyne/cm2, 24 hours), and (5) HiLSS followed by CRP stimulation. Results. AA HUVECs had significantly higher FcγRIIB receptor expression under both basal and CRP incubation conditions. Blocking FcγRIIB receptor significantly attenuated the CRP-induced decrements in eNOS expression only in AA HUVECs. Finally, HiLSS significantly counteracted CRP-induced effects. Conclusion. Understanding potential racial differences in endothelial function is important to improve CVD prevention. Our results shed light on FcγRIIB receptor as a potential contributor to racial differences in endothelial function in AA.http://dx.doi.org/10.1155/2021/6687250 |
| spellingShingle | Chenyi Ling Marc D. Cook Heather Grimm Maitha Aldokhayyil Dulce Gomez Michael Brown The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells Mediators of Inflammation |
| title | The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells |
| title_full | The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells |
| title_fullStr | The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells |
| title_full_unstemmed | The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells |
| title_short | The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells |
| title_sort | effect of race and shear stress on crp induced responses in endothelial cells |
| url | http://dx.doi.org/10.1155/2021/6687250 |
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