Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria
Abstract In natural environments, microbes are typically non‐dividing and gauge when nutrients permit division. Current models are phenomenological and specific to nutrient‐rich, exponentially growing cells, thus cannot predict the first division under limiting nutrient availability. To assess this...
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| Format: | Article |
| Language: | English |
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Springer Nature
2018-11-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.15252/msb.20188623 |
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| _version_ | 1849341961780068352 |
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| author | Karthik Sekar Roberto Rusconi John T Sauls Tobias Fuhrer Elad Noor Jen Nguyen Vicente I Fernandez Marieke F Buffing Michael Berney Suckjoon Jun Roman Stocker Uwe Sauer |
| author_facet | Karthik Sekar Roberto Rusconi John T Sauls Tobias Fuhrer Elad Noor Jen Nguyen Vicente I Fernandez Marieke F Buffing Michael Berney Suckjoon Jun Roman Stocker Uwe Sauer |
| author_sort | Karthik Sekar |
| collection | DOAJ |
| description | Abstract In natural environments, microbes are typically non‐dividing and gauge when nutrients permit division. Current models are phenomenological and specific to nutrient‐rich, exponentially growing cells, thus cannot predict the first division under limiting nutrient availability. To assess this regime, we supplied starving Escherichia coli with glucose pulses at increasing frequencies. Real‐time metabolomics and microfluidic single‐cell microscopy revealed unexpected, rapid protein, and nucleic acid synthesis already from minuscule glucose pulses in non‐dividing cells. Additionally, the lag time to first division shortened as pulsing frequency increased. We pinpointed division timing and dependence on nutrient frequency to the changing abundance of the division protein FtsZ. A dynamic, mechanistic model quantitatively relates lag time to FtsZ synthesis from nutrient pulses and FtsZ protease‐dependent degradation. Lag time changed in model‐congruent manners, when we experimentally modulated the synthesis or degradation of FtsZ. Thus, limiting abundance of FtsZ can quantitatively predict timing of the first cell division. |
| format | Article |
| id | doaj-art-12cfbcdd67a24e8387cbbf577a075f3f |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2018-11-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-12cfbcdd67a24e8387cbbf577a075f3f2025-08-20T03:43:31ZengSpringer NatureMolecular Systems Biology1744-42922018-11-01141111410.15252/msb.20188623Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteriaKarthik Sekar0Roberto Rusconi1John T Sauls2Tobias Fuhrer3Elad Noor4Jen Nguyen5Vicente I Fernandez6Marieke F Buffing7Michael Berney8Suckjoon Jun9Roman Stocker10Uwe Sauer11Department of Biology, Institute of Molecular Systems Biology, ETH ZurichDepartment of Civil, Environmental and Geomatic Engineering, Institute of Environmental Engineering, ETH ZurichDepartment of Physics, University of California at San DiegoDepartment of Biology, Institute of Molecular Systems Biology, ETH ZurichDepartment of Biology, Institute of Molecular Systems Biology, ETH ZurichDepartment of Civil, Environmental and Geomatic Engineering, Institute of Environmental Engineering, ETH ZurichDepartment of Civil, Environmental and Geomatic Engineering, Institute of Environmental Engineering, ETH ZurichDepartment of Biology, Institute of Molecular Systems Biology, ETH ZurichDepartment of Microbiology and Immunology, Albert Einstein College of MedicineDepartment of Physics, University of California at San DiegoDepartment of Civil, Environmental and Geomatic Engineering, Institute of Environmental Engineering, ETH ZurichDepartment of Biology, Institute of Molecular Systems Biology, ETH ZurichAbstract In natural environments, microbes are typically non‐dividing and gauge when nutrients permit division. Current models are phenomenological and specific to nutrient‐rich, exponentially growing cells, thus cannot predict the first division under limiting nutrient availability. To assess this regime, we supplied starving Escherichia coli with glucose pulses at increasing frequencies. Real‐time metabolomics and microfluidic single‐cell microscopy revealed unexpected, rapid protein, and nucleic acid synthesis already from minuscule glucose pulses in non‐dividing cells. Additionally, the lag time to first division shortened as pulsing frequency increased. We pinpointed division timing and dependence on nutrient frequency to the changing abundance of the division protein FtsZ. A dynamic, mechanistic model quantitatively relates lag time to FtsZ synthesis from nutrient pulses and FtsZ protease‐dependent degradation. Lag time changed in model‐congruent manners, when we experimentally modulated the synthesis or degradation of FtsZ. Thus, limiting abundance of FtsZ can quantitatively predict timing of the first cell division.https://doi.org/10.15252/msb.20188623divisionEscherichia coliFtsZprotein degradationstarvation |
| spellingShingle | Karthik Sekar Roberto Rusconi John T Sauls Tobias Fuhrer Elad Noor Jen Nguyen Vicente I Fernandez Marieke F Buffing Michael Berney Suckjoon Jun Roman Stocker Uwe Sauer Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria Molecular Systems Biology division Escherichia coli FtsZ protein degradation starvation |
| title | Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria |
| title_full | Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria |
| title_fullStr | Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria |
| title_full_unstemmed | Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria |
| title_short | Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria |
| title_sort | synthesis and degradation of ftsz quantitatively predict the first cell division in starved bacteria |
| topic | division Escherichia coli FtsZ protein degradation starvation |
| url | https://doi.org/10.15252/msb.20188623 |
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