Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria
Purpose: This study aimed to utilize genetically engineered <i>Bacillus licheniformis</i> for the production of ergothioneine (EGT). Given the value of EGT and the application of <i>Bacillus licheniformis</i> in enzyme preparation production, we cloned the key enzymes (EanA a...
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| author | Zhe Liu Fengxu Xiao Yupeng Zhang Jiawei Lu Youran Li Guiyang Shi |
| author_facet | Zhe Liu Fengxu Xiao Yupeng Zhang Jiawei Lu Youran Li Guiyang Shi |
| author_sort | Zhe Liu |
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| description | Purpose: This study aimed to utilize genetically engineered <i>Bacillus licheniformis</i> for the production of ergothioneine (EGT). Given the value of EGT and the application of <i>Bacillus licheniformis</i> in enzyme preparation production, we cloned the key enzymes (EanA and EanB) from <i>Chlorbium limicola</i>. Through gene alignment, new ergothioneine synthase genes (EanAN and EanBN) were identified and then expressed in <i>Bacillus licheniformis</i> to construct strains. Additionally, we investigated the factors influencing the yield of EGT and made a comparison with <i>Escherichia coli</i>. Methods: The relevant genes were cloned and transferred into <i>Bacillus licheniformis</i>. Fermentation experiments were conducted under different conditions for yield analysis, and the stability of this bacterium was also evaluated simultaneously. Results: The constructed strains were capable of producing EGT. Specifically, the yield of the EanANBN strain reached (643.8 ± 135) mg/L, and its stability was suitable for continuous production. Conclusions: Genetically engineered <i>Bacillus licheniformis</i> demonstrates potential in the industrial-scale production of EGT. Compared with <i>Escherichia coli</i>, it has advantages, thus opening up new possibilities for the application and market supply of EGT. |
| format | Article |
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| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Metabolites |
| spelling | doaj-art-12be8b7b10a04b3f99985c3da4560f5d2025-01-24T13:41:16ZengMDPI AGMetabolites2218-19892025-01-011514510.3390/metabo15010045Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic BacteriaZhe Liu0Fengxu Xiao1Yupeng Zhang2Jiawei Lu3Youran Li4Guiyang Shi5School of Biotechnology, Key Laboratory of Carbohydrate Chemistry, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, ChinaSchool of Biotechnology, Key Laboratory of Carbohydrate Chemistry, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, ChinaSchool of Biotechnology, Key Laboratory of Carbohydrate Chemistry, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, ChinaSchool of Biotechnology, Key Laboratory of Carbohydrate Chemistry, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, ChinaSchool of Biotechnology, Key Laboratory of Carbohydrate Chemistry, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, ChinaSchool of Biotechnology, Key Laboratory of Carbohydrate Chemistry, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, ChinaPurpose: This study aimed to utilize genetically engineered <i>Bacillus licheniformis</i> for the production of ergothioneine (EGT). Given the value of EGT and the application of <i>Bacillus licheniformis</i> in enzyme preparation production, we cloned the key enzymes (EanA and EanB) from <i>Chlorbium limicola</i>. Through gene alignment, new ergothioneine synthase genes (EanAN and EanBN) were identified and then expressed in <i>Bacillus licheniformis</i> to construct strains. Additionally, we investigated the factors influencing the yield of EGT and made a comparison with <i>Escherichia coli</i>. Methods: The relevant genes were cloned and transferred into <i>Bacillus licheniformis</i>. Fermentation experiments were conducted under different conditions for yield analysis, and the stability of this bacterium was also evaluated simultaneously. Results: The constructed strains were capable of producing EGT. Specifically, the yield of the EanANBN strain reached (643.8 ± 135) mg/L, and its stability was suitable for continuous production. Conclusions: Genetically engineered <i>Bacillus licheniformis</i> demonstrates potential in the industrial-scale production of EGT. Compared with <i>Escherichia coli</i>, it has advantages, thus opening up new possibilities for the application and market supply of EGT.https://www.mdpi.com/2218-1989/15/1/45ergothioneinewhole-cell transformation<i>Bacillus licheniformis</i>amino acidsmethyltransferasessulfur transferases |
| spellingShingle | Zhe Liu Fengxu Xiao Yupeng Zhang Jiawei Lu Youran Li Guiyang Shi Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria Metabolites ergothioneine whole-cell transformation <i>Bacillus licheniformis</i> amino acids methyltransferases sulfur transferases |
| title | Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria |
| title_full | Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria |
| title_fullStr | Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria |
| title_full_unstemmed | Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria |
| title_short | Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria |
| title_sort | heterologous and high production of ergothioneine in i bacillus licheniformis i by using genes from anaerobic bacteria |
| topic | ergothioneine whole-cell transformation <i>Bacillus licheniformis</i> amino acids methyltransferases sulfur transferases |
| url | https://www.mdpi.com/2218-1989/15/1/45 |
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