Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria

Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria

Purpose: This study aimed to utilize genetically engineered <i>Bacillus licheniformis</i> for the production of ergothioneine (EGT). Given the value of EGT and the application of <i>Bacillus licheniformis</i> in enzyme preparation production, we cloned the key enzymes (EanA a...

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Bibliographic Details
Main Authors: Zhe Liu, Fengxu Xiao, Yupeng Zhang, Jiawei Lu, Youran Li, Guiyang Shi
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Metabolites
Subjects:
ergothioneine
whole-cell transformation
<i>Bacillus licheniformis</i>
amino acids
methyltransferases
sulfur transferases
Online Access:https://www.mdpi.com/2218-1989/15/1/45
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Summary:Purpose: This study aimed to utilize genetically engineered <i>Bacillus licheniformis</i> for the production of ergothioneine (EGT). Given the value of EGT and the application of <i>Bacillus licheniformis</i> in enzyme preparation production, we cloned the key enzymes (EanA and EanB) from <i>Chlorbium limicola</i>. Through gene alignment, new ergothioneine synthase genes (EanAN and EanBN) were identified and then expressed in <i>Bacillus licheniformis</i> to construct strains. Additionally, we investigated the factors influencing the yield of EGT and made a comparison with <i>Escherichia coli</i>. Methods: The relevant genes were cloned and transferred into <i>Bacillus licheniformis</i>. Fermentation experiments were conducted under different conditions for yield analysis, and the stability of this bacterium was also evaluated simultaneously. Results: The constructed strains were capable of producing EGT. Specifically, the yield of the EanANBN strain reached (643.8 ± 135) mg/L, and its stability was suitable for continuous production. Conclusions: Genetically engineered <i>Bacillus licheniformis</i> demonstrates potential in the industrial-scale production of EGT. Compared with <i>Escherichia coli</i>, it has advantages, thus opening up new possibilities for the application and market supply of EGT.
ISSN:2218-1989

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