Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition
Abstract Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by immune dysregulation and excessive cytokine production. This study aimed to explore the potential of Camellia sinensis L. water extract (CSE) as a treatment for AD by the impact of CSE on inflammatory responses i...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86678-5 |
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| author | Min Jung Kim Ye Jin Yang Ga-Yul Min Ji Woong Heo Jae Dong Son Young Zoo You Hun Hwan Kim Gon Sup Kim Hu-Jang Lee Ju-Hye Yang Kwang Il Park |
| author_facet | Min Jung Kim Ye Jin Yang Ga-Yul Min Ji Woong Heo Jae Dong Son Young Zoo You Hun Hwan Kim Gon Sup Kim Hu-Jang Lee Ju-Hye Yang Kwang Il Park |
| author_sort | Min Jung Kim |
| collection | DOAJ |
| description | Abstract Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by immune dysregulation and excessive cytokine production. This study aimed to explore the potential of Camellia sinensis L. water extract (CSE) as a treatment for AD by the impact of CSE on inflammatory responses in keratinocytes, particularly concerning the production of inflammatory cytokines and the modulation of signaling pathways relevant to AD pathogenesis. CSE was obtained via hot water extraction from Camellia sinensis L. Ultra-high-performance liquid chromatography (UPLC) analyzed catechin and caffeine content. Cell viability was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), polyphenol and flavonoid content were determined. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay measured antioxidant activity. Enzyme-Linked Immunosorbent Assay (ELISA), western blotting, and Immunofluorescence (IF) assays examined cytokines, pathways, and protein localization, respectively. Molecular docking assessed compound binding with inflammation-related proteins. UPLC identified six CSE components including epigallocatechin (EGC) epicatechin (EC), caffeine (CF), catechin (C), epigallocatechin gallate (EGCG), and epicatechin gallate (ECG). CSE demonstrated a significant reduction in the production of inflammatory cytokines interleukin (IL)-2 and IL-6 in TNF-α/IFN-γ activated keratinocytes. Treatment with CSE inhibited the mitogen-activated protein kinase (MAPK) pathway, which resulted in decreased phosphorylation of p38, Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK). Exposure of TNF-α/IFN-γ- stimulated human keratinocytes (HaCaT) cells to CSE resulted in a 200 µg/mL dependent inhibition of p65 and signal transducer and activator of transcription 1 (STAT-1) translocation from the cytosol to the nucleus, as confirmed through immunofluorescence (IF) staining. Molecular docking simulations provided insights into the underlying mechanisms of CSE action, which supported its potential as a therapeutic agent for AD. CSE might be a potential candidate for its therapeutic efficacy for inflammatory skin conditions like AD. Thus, based on this evidence, the authors suggest that CSE should be studied further for its anti-inflammatory activities and topical application in the treatment of AD. |
| format | Article |
| id | doaj-art-11bb27c01aee4332b0ad403740033944 |
| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-11bb27c01aee4332b0ad4037400339442025-01-19T12:18:46ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-025-86678-5Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibitionMin Jung Kim0Ye Jin Yang1Ga-Yul Min2Ji Woong Heo3Jae Dong Son4Young Zoo You5Hun Hwan Kim6Gon Sup Kim7Hu-Jang Lee8Ju-Hye Yang9Kwang Il Park10Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National UniversityDepartment of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National UniversityKorean Medicine (KM) Application Center, Korea Institute of Oriental MedicineDepartment of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National UniversityDepartment of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National UniversityDepartment of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National UniversityDepartment of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National UniversityDepartment of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National UniversityDepartment of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National UniversityKorean Medicine (KM) Application Center, Korea Institute of Oriental MedicineDepartment of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National UniversityAbstract Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by immune dysregulation and excessive cytokine production. This study aimed to explore the potential of Camellia sinensis L. water extract (CSE) as a treatment for AD by the impact of CSE on inflammatory responses in keratinocytes, particularly concerning the production of inflammatory cytokines and the modulation of signaling pathways relevant to AD pathogenesis. CSE was obtained via hot water extraction from Camellia sinensis L. Ultra-high-performance liquid chromatography (UPLC) analyzed catechin and caffeine content. Cell viability was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), polyphenol and flavonoid content were determined. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay measured antioxidant activity. Enzyme-Linked Immunosorbent Assay (ELISA), western blotting, and Immunofluorescence (IF) assays examined cytokines, pathways, and protein localization, respectively. Molecular docking assessed compound binding with inflammation-related proteins. UPLC identified six CSE components including epigallocatechin (EGC) epicatechin (EC), caffeine (CF), catechin (C), epigallocatechin gallate (EGCG), and epicatechin gallate (ECG). CSE demonstrated a significant reduction in the production of inflammatory cytokines interleukin (IL)-2 and IL-6 in TNF-α/IFN-γ activated keratinocytes. Treatment with CSE inhibited the mitogen-activated protein kinase (MAPK) pathway, which resulted in decreased phosphorylation of p38, Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK). Exposure of TNF-α/IFN-γ- stimulated human keratinocytes (HaCaT) cells to CSE resulted in a 200 µg/mL dependent inhibition of p65 and signal transducer and activator of transcription 1 (STAT-1) translocation from the cytosol to the nucleus, as confirmed through immunofluorescence (IF) staining. Molecular docking simulations provided insights into the underlying mechanisms of CSE action, which supported its potential as a therapeutic agent for AD. CSE might be a potential candidate for its therapeutic efficacy for inflammatory skin conditions like AD. Thus, based on this evidence, the authors suggest that CSE should be studied further for its anti-inflammatory activities and topical application in the treatment of AD.https://doi.org/10.1038/s41598-025-86678-5Anti-inflammationAntioxidantAtopic dermatitisCamellia sinensis L.Ultra performance liquid chromatography |
| spellingShingle | Min Jung Kim Ye Jin Yang Ga-Yul Min Ji Woong Heo Jae Dong Son Young Zoo You Hun Hwan Kim Gon Sup Kim Hu-Jang Lee Ju-Hye Yang Kwang Il Park Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition Scientific Reports Anti-inflammation Antioxidant Atopic dermatitis Camellia sinensis L. Ultra performance liquid chromatography |
| title | Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition |
| title_full | Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition |
| title_fullStr | Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition |
| title_full_unstemmed | Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition |
| title_short | Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition |
| title_sort | anti inflammatory and antioxidant properties of camellia sinensis l extract as a potential therapeutic for atopic dermatitis through nf κb pathway inhibition |
| topic | Anti-inflammation Antioxidant Atopic dermatitis Camellia sinensis L. Ultra performance liquid chromatography |
| url | https://doi.org/10.1038/s41598-025-86678-5 |
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