The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence method
The vaginal microbiota plays an important role in reproductive health, especially in the process of artificial insemination. The imbalance of microbiota may affect pregnancy outcomes. Therefore, this study aims to explore the composition of vaginal microbiota and its impact on artificial inseminatio...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | SLAS Technology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2472630324001195 |
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| author | Yanling Xing Wei Wang Na Wan Dongmei Zhang Mei Shan Guan Wang |
| author_facet | Yanling Xing Wei Wang Na Wan Dongmei Zhang Mei Shan Guan Wang |
| author_sort | Yanling Xing |
| collection | DOAJ |
| description | The vaginal microbiota plays an important role in reproductive health, especially in the process of artificial insemination. The imbalance of microbiota may affect pregnancy outcomes. Therefore, this study aims to explore the composition of vaginal microbiota and its impact on artificial insemination pregnancy outcomes, combined with microscopic images and PCR fluorescence methods, in order to provide scientific basis for improving the pregnancy outcomes of husband sperm artificial insemination. This study included female patients who underwent artificial insemination with husband's sperm and collected reproductive tract samples. By observing the microbial community morphology in the sample under a microscope and analyzing microbial DNA using PCR fluorescence method, the microbial ecological status is evaluated. Among women with successful pregnancy, the proportion of beneficial bacteria (such as lactic acid bacteria) is higher, while the abundance of pathogenic bacteria is significantly reduced. Specific microbial markers detected by PCR fluorescence method are positively correlated with pregnancy rate. Therefore, the microecological state of the female reproductive tract has a significant impact on the pregnancy outcome of artificial insemination. Maintaining a good microecological balance, especially increasing the proportion of beneficial bacteria, can help improve the success rate of artificial insemination. |
| format | Article |
| id | doaj-art-11a85c5d19a147f398148594028375a0 |
| institution | Kabale University |
| issn | 2472-6303 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | SLAS Technology |
| spelling | doaj-art-11a85c5d19a147f398148594028375a02025-01-23T05:27:28ZengElsevierSLAS Technology2472-63032025-02-0130100242The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence methodYanling Xing0Wei Wang1Na Wan2Dongmei Zhang3Mei Shan4Guan Wang5Department of Gynecology, Heilongjiang Provincial Hospital, Harbin, 150000, Heilongjiang Province, ChinaDepartment of Reproductive medicine, Heilongjiang Provincial Hospital, Harbin, 150000, Heilongjiang Province, ChinaDepartment of Gynecology, Heilongjiang Provincial Hospital, Harbin, 150000, Heilongjiang Province, ChinaDepartment of Reproductive medicine, Heilongjiang Provincial Hospital, Harbin, 150000, Heilongjiang Province, ChinaDepartment of Reproductive medicine, Heilongjiang Provincial Hospital, Harbin, 150000, Heilongjiang Province, ChinaDepartment of Reproductive medicine, Heilongjiang Provincial Hospital, Harbin, 150000, Heilongjiang Province, China; Corresponding author.The vaginal microbiota plays an important role in reproductive health, especially in the process of artificial insemination. The imbalance of microbiota may affect pregnancy outcomes. Therefore, this study aims to explore the composition of vaginal microbiota and its impact on artificial insemination pregnancy outcomes, combined with microscopic images and PCR fluorescence methods, in order to provide scientific basis for improving the pregnancy outcomes of husband sperm artificial insemination. This study included female patients who underwent artificial insemination with husband's sperm and collected reproductive tract samples. By observing the microbial community morphology in the sample under a microscope and analyzing microbial DNA using PCR fluorescence method, the microbial ecological status is evaluated. Among women with successful pregnancy, the proportion of beneficial bacteria (such as lactic acid bacteria) is higher, while the abundance of pathogenic bacteria is significantly reduced. Specific microbial markers detected by PCR fluorescence method are positively correlated with pregnancy rate. Therefore, the microecological state of the female reproductive tract has a significant impact on the pregnancy outcome of artificial insemination. Maintaining a good microecological balance, especially increasing the proportion of beneficial bacteria, can help improve the success rate of artificial insemination.http://www.sciencedirect.com/science/article/pii/S2472630324001195Microscope imagePCR fluorescence methodVaginal microbiotaArtificial insemination of husband's spermPregnancy outcome |
| spellingShingle | Yanling Xing Wei Wang Na Wan Dongmei Zhang Mei Shan Guan Wang The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence method SLAS Technology Microscope image PCR fluorescence method Vaginal microbiota Artificial insemination of husband's sperm Pregnancy outcome |
| title | The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence method |
| title_full | The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence method |
| title_fullStr | The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence method |
| title_full_unstemmed | The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence method |
| title_short | The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence method |
| title_sort | influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband s sperm based on microscope images combined with pcr fluorescence method |
| topic | Microscope image PCR fluorescence method Vaginal microbiota Artificial insemination of husband's sperm Pregnancy outcome |
| url | http://www.sciencedirect.com/science/article/pii/S2472630324001195 |
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