Protective effects of peptide KSPLY derived from Hericium erinaceus on H2O2-induced oxidative damage in HepG2 cells
Reactive oxygen species (ROS)-induced oxidative damage is strongly associated with the pathogenesis of chronic diseases, and natural antioxidant peptides have good abilities of scavenging ROS. The antioxidant activity of peptide Lys-Ser-Pro-Leu-Tyr (KSPLY) derived from Hericium erinaceus remains unc...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000411 |
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| author | Zhengli Xu Qiuhui Hu Minhao Xie Jianhui Liu Anxiang Su Hui Xu Wenjian Yang |
| author_facet | Zhengli Xu Qiuhui Hu Minhao Xie Jianhui Liu Anxiang Su Hui Xu Wenjian Yang |
| author_sort | Zhengli Xu |
| collection | DOAJ |
| description | Reactive oxygen species (ROS)-induced oxidative damage is strongly associated with the pathogenesis of chronic diseases, and natural antioxidant peptides have good abilities of scavenging ROS. The antioxidant activity of peptide Lys-Ser-Pro-Leu-Tyr (KSPLY) derived from Hericium erinaceus remains unclear. In the present study, the antioxidant effect and mechanism of KSPLY on H2O2-induced oxidative damage in HepG2 cells were investigated. The results indicated that KSPLY exhibited the antioxidant capacity in H2O2-induced HepG2 cells by enhancing superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities. In comparison with the H2O2-treated damage group, the apoptosis rate, ROS level, and malondialdehyde (MDA) content of HepG2 cells treated with KSPLY were significantly decreased. The H. Erinaceus-derived peptide KSPLY pretreatment promoted the expression of detoxification and antioxidant enzymes via the Keap1/Nrf2 signal pathway, thereby inhibiting the generation of ROS and MDA. In conclusion, the H. erinaceus-derived peptide KSPLY effectively protected HepG2 cells against H2O2-induced oxidative damage, and it provided a theoretical basis for the further development of new natural antioxidants. |
| format | Article |
| id | doaj-art-119d4316de3e438daea0e4243c7ef444 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-119d4316de3e438daea0e4243c7ef4442025-02-03T00:27:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112518931904Protective effects of peptide KSPLY derived from Hericium erinaceus on H2O2-induced oxidative damage in HepG2 cellsZhengli Xu0Qiuhui Hu1Minhao Xie2Jianhui Liu3Anxiang Su4Hui Xu5Wenjian Yang6College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 21023 ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 21023 ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 21023 ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 21023 ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 21023 ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 21023 ChinaCorrespondenece to: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China,; College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 21023 ChinaReactive oxygen species (ROS)-induced oxidative damage is strongly associated with the pathogenesis of chronic diseases, and natural antioxidant peptides have good abilities of scavenging ROS. The antioxidant activity of peptide Lys-Ser-Pro-Leu-Tyr (KSPLY) derived from Hericium erinaceus remains unclear. In the present study, the antioxidant effect and mechanism of KSPLY on H2O2-induced oxidative damage in HepG2 cells were investigated. The results indicated that KSPLY exhibited the antioxidant capacity in H2O2-induced HepG2 cells by enhancing superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities. In comparison with the H2O2-treated damage group, the apoptosis rate, ROS level, and malondialdehyde (MDA) content of HepG2 cells treated with KSPLY were significantly decreased. The H. Erinaceus-derived peptide KSPLY pretreatment promoted the expression of detoxification and antioxidant enzymes via the Keap1/Nrf2 signal pathway, thereby inhibiting the generation of ROS and MDA. In conclusion, the H. erinaceus-derived peptide KSPLY effectively protected HepG2 cells against H2O2-induced oxidative damage, and it provided a theoretical basis for the further development of new natural antioxidants.http://www.sciencedirect.com/science/article/pii/S2213453023000411Antioxidant peptide KSPLYProtective effectKeap1/Nrf2 signaling pathway |
| spellingShingle | Zhengli Xu Qiuhui Hu Minhao Xie Jianhui Liu Anxiang Su Hui Xu Wenjian Yang Protective effects of peptide KSPLY derived from Hericium erinaceus on H2O2-induced oxidative damage in HepG2 cells Food Science and Human Wellness Antioxidant peptide KSPLY Protective effect Keap1/Nrf2 signaling pathway |
| title | Protective effects of peptide KSPLY derived from Hericium erinaceus on H2O2-induced oxidative damage in HepG2 cells |
| title_full | Protective effects of peptide KSPLY derived from Hericium erinaceus on H2O2-induced oxidative damage in HepG2 cells |
| title_fullStr | Protective effects of peptide KSPLY derived from Hericium erinaceus on H2O2-induced oxidative damage in HepG2 cells |
| title_full_unstemmed | Protective effects of peptide KSPLY derived from Hericium erinaceus on H2O2-induced oxidative damage in HepG2 cells |
| title_short | Protective effects of peptide KSPLY derived from Hericium erinaceus on H2O2-induced oxidative damage in HepG2 cells |
| title_sort | protective effects of peptide ksply derived from hericium erinaceus on h2o2 induced oxidative damage in hepg2 cells |
| topic | Antioxidant peptide KSPLY Protective effect Keap1/Nrf2 signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000411 |
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