Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada
Rectal swabs from 155 sheep and 252 goats from Grenada were evaluated to determine the prevalence of Campylobacter spp., antibiotic resistance, and multilocus sequence types. Fifteen Campylobacter isolates were obtained (14 C. jejuni and 1 C. col...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2014/212864 |
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| author | Diana M. Stone Yogesh Chander Aschalew Z. Bekele Sagar M. Goyal Harry Hariharan Keshaw Tiwari Alfred Chikweto Ravindra Sharma |
| author_facet | Diana M. Stone Yogesh Chander Aschalew Z. Bekele Sagar M. Goyal Harry Hariharan Keshaw Tiwari Alfred Chikweto Ravindra Sharma |
| author_sort | Diana M. Stone |
| collection | DOAJ |
| description | Rectal swabs from 155 sheep and 252 goats from Grenada were evaluated to
determine the prevalence of Campylobacter spp., antibiotic resistance,
and multilocus sequence types. Fifteen Campylobacter isolates were obtained
(14 C. jejuni and 1 C. coli). The prevalence (3.7%) did not differ
significantly between sheep (4.5%) and goats (3.2%). Among the seven antimicrobials
tested, resistance was only detected for tetracycline (30.8%) and metronidazole (38.5%).
Campylobacter isolates showed no significant difference between sheep and goats for
type of antimicrobial resistance or percent of resistant isolates. Twelve of the isolates were
successfully genotyped consisting of four recognized clonal complexes and three novel sequence
types. Importantly, one isolate from one goat was identified as the C. jejuni sequence
type-8, a zoonotic and tetracycline-resistant clone reported to be a highly virulent clone
associated with ovine abortion in the USA. Although most samples were from comingled sheep
and goat production units, there were no shared sequence types between these two host species.
None of the sequence types identified in this study have previously been reported in poultry in Grenada,
suggesting sheep- and goat-specific Campylobacter clones in Grenada. This is the
first report of genotyping of Campylobacter isolates from sheep and goats in the Eastern Caribbean. |
| format | Article |
| id | doaj-art-119bf00b06ea41fdbe81f25eaf654bc4 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-119bf00b06ea41fdbe81f25eaf654bc42025-02-03T06:12:21ZengWileyVeterinary Medicine International2090-81132042-00482014-01-01201410.1155/2014/212864212864Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in GrenadaDiana M. Stone0Yogesh Chander1Aschalew Z. Bekele2Sagar M. Goyal3Harry Hariharan4Keshaw Tiwari5Alfred Chikweto6Ravindra Sharma7School of Veterinary Medicine, St. George’s University, P.O. Box 7, St. George, GrenadaDepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, USADepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, USADepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, USASchool of Veterinary Medicine, St. George’s University, P.O. Box 7, St. George, GrenadaSchool of Veterinary Medicine, St. George’s University, P.O. Box 7, St. George, GrenadaSchool of Veterinary Medicine, St. George’s University, P.O. Box 7, St. George, GrenadaSchool of Veterinary Medicine, St. George’s University, P.O. Box 7, St. George, GrenadaRectal swabs from 155 sheep and 252 goats from Grenada were evaluated to determine the prevalence of Campylobacter spp., antibiotic resistance, and multilocus sequence types. Fifteen Campylobacter isolates were obtained (14 C. jejuni and 1 C. coli). The prevalence (3.7%) did not differ significantly between sheep (4.5%) and goats (3.2%). Among the seven antimicrobials tested, resistance was only detected for tetracycline (30.8%) and metronidazole (38.5%). Campylobacter isolates showed no significant difference between sheep and goats for type of antimicrobial resistance or percent of resistant isolates. Twelve of the isolates were successfully genotyped consisting of four recognized clonal complexes and three novel sequence types. Importantly, one isolate from one goat was identified as the C. jejuni sequence type-8, a zoonotic and tetracycline-resistant clone reported to be a highly virulent clone associated with ovine abortion in the USA. Although most samples were from comingled sheep and goat production units, there were no shared sequence types between these two host species. None of the sequence types identified in this study have previously been reported in poultry in Grenada, suggesting sheep- and goat-specific Campylobacter clones in Grenada. This is the first report of genotyping of Campylobacter isolates from sheep and goats in the Eastern Caribbean.http://dx.doi.org/10.1155/2014/212864 |
| spellingShingle | Diana M. Stone Yogesh Chander Aschalew Z. Bekele Sagar M. Goyal Harry Hariharan Keshaw Tiwari Alfred Chikweto Ravindra Sharma Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada Veterinary Medicine International |
| title | Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada |
| title_full | Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada |
| title_fullStr | Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada |
| title_full_unstemmed | Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada |
| title_short | Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada |
| title_sort | genotypes antibiotic resistance and st 8 genetic clone in campylobacter isolates from sheep and goats in grenada |
| url | http://dx.doi.org/10.1155/2014/212864 |
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